Mouse CXCL2/MIP-2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY452
DY452-05
Ancillary Products Available
CXCL2/GRO beta/MIP-2/CINC-3 ELISA Kit ELISA
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Product Details
Procedure
Citations (87)
FAQs
Supplemental Products
Reviews (4)

Mouse CXCL2/MIP-2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For five or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse MIP-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse CXCL2 / GRO beta / MIP-2 / CINC-3 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL2/GRO beta/MIP-2/CINC-3

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.

Entrez Gene IDs:
2920 (Human); 20310 (Mouse); 114105 (Rat)
Alternate Names:
chemokine (C-X-C motif) ligand 2; CINC-2a; CINC3; CINC-3; C-X-C Motif Chemokine 2; CXCL2; GRO beta; GRO2 oncogene; GRO2; GROB; Gro-beta; Growth-regulated protein beta; Macrophage inflammatory protein 2-alpha; melanoma growth stimulatory activity beta; MGSA beta; MGSA-b; MGSA-beta; MIP2; MIP-2; MIP2A; MIP-2a; MIP2-alpha; SCYB2

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CXCL2/MIP-2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

87 Citations: Showing 1 - 10
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  1. Role of a fluid-phase PRR in fighting an intracellular pathogen: PTX3 in Shigella infection
    Authors: V Ciancarell, L Lembo-Fazi, I Paciello, AK Bruno, S Jaillon, S Berardi, M Barbagallo, S Meron-Suda, D Cohen, A Molinaro, G Rossi, C Garlanda, ML Bernardini
    PLoS Pathog., 2018;14(12):e1007469.
    Species: Mouse
    Sample Types: Tissue Homegenate
  2. Intermittent Hypoxia Activates Duration-Dependent Protective and Injurious Mechanisms in Mouse Lung Endothelial Cells
    Authors: P Wohlrab, L Soto-Gonza, T Benesch, MP Winter, IM Lang, K Markstalle, V Tretter, KU Klein
    Front Physiol, 2018;9(0):1754.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  3. Lipoteichoic acid anchor triggers Mincle to drive protective immunity against invasive group A Streptococcus infection
    Authors: T Imai, T Matsumura, S Mayer-Lamb, CA Wells, E Ishikawa, SK Butcher, TC Barnett, MJ Walker, A Imamura, H Ishida, T Ikebe, T Miyamoto, M Ato, S Ohga, B Lepenies, NM van Sorge, S Yamasaki
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  4. Extracellular ATP is a danger signal activating P2X7 receptor in a LPS mediated inflammation (ARDS/ALI)
    Authors: S Cicko, TC Köhler, CK Ayata, T Müller, N Ehrat, A Meyer, M Hossfeld, A Zech, F Di Virgili, M Idzko
    Oncotarget, 2018;9(55):30635-30648.
    Species: Mouse
    Sample Types: BALF
  5. Host-inherent variability influences the transcriptional response of Staphylococcus aureus during in vivo infection
    Authors: R Th„nert, O Goldmann, A Beineke, E Medina
    Nat Commun, 2017;8(0):14268.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Chlamydial Lipoproteins Stimulate Toll-Like Receptors 1/2 Mediated Inflammatory Responses through MyD88-Dependent Pathway
    Authors: Y Wang, Q Liu, D Chen, J Guan, L Ma, G Zhong, H Shu, X Wu
    Front Microbiol, 2017;8(0):78.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Deletion of the Chemokine Binding Protein Gene from the Parapoxvirus Orf Virus Reduces Virulence and Pathogenesis in Sheep
    Authors: SB Fleming, C McCaughan, Z Lateef, A Dunn, LM Wise, NC Real, AA Mercer
    Front Microbiol, 2017;8(0):46.
    Species: Mouse
    Sample Types: Protein
  8. Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response
    Authors: M Lynch, TA Walsh, I Marszalows, AE Webb, M MacAogain, TR Rogers, H Windle, D Kelleher, MJ O'Connell, CE Loscher
    BMC Evol. Biol, 2017;17(1):90.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  9. ?-bisabolol-loaded lipid-core nanocapsules reduce lipopolysaccharide-induced pulmonary inflammation in mice
    Authors: APL D'Almeida, MT Pacheco de, ÉT de Souza, D de Sá Cout, BT Ciambarell, CR Gomes, T Terroso, SS Guterres, AR Pohlmann, PM Silva, MA Martins, A Bernardi
    Int J Nanomedicine, 2017;12(0):4479-4491.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. Theranostic Nucleic Acid Binding Nanoprobe Exerts Anti-inflammatory and Cytoprotective Effects in Ischemic Injury
    Authors: HH Chen, H Yuan, H Cho, Y Feng, S Ngoy, AT Kumar, R Liao, W Chao, L Josephson, DE Sosnovik
    Theranostics, 2017;7(4):814-825.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  11. Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis
    Authors: K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri
    PLoS Pathog., 2017;13(8):e1006513.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection
    Authors: EE Hornick, B Banoth, AM Miller, ZR Zacharias, N Jain, ME Wilson, KN Gibson-Cor, KL Legge, GA Bishop, FS Sutterwala, SL Cassel
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. Acinetobacter baumannii Lipopolysaccharide Influences Adipokine Expression in 3T3-L1 Adipocytes
    Authors: Y Unno, Y Sato, S Nishida, A Nakano, R Nakano, T Ubagai, Y Ono
    Mediators Inflamm., 2017;2017(0):9039302.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. C1P Attenuates Lipopolysaccharide-Induced Acute Lung Injury by Preventing NF-?B Activation in Neutrophils
    J Immunol, 2016;196(5):2319-26.
    Species: Mouse
    Sample Types: BALF
  15. NK cells regulate CXCR2+ neutrophil recruitment during acute lung injury
    J Leukoc Biol, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. Anti-Inflammatory Effects of Rosiglitazone in Obesity-Impaired Wound Healing Depend on Adipocyte Differentiation
    PLoS ONE, 2016;11(12):e0168562.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  17. The Carbohydrate Lectin Receptor, Dectin-1, Mediates Immune Response to Exserohilum rostratum
    Infect. Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. The Fli-1 transcription factor is a critical regulator for controlling the expression of chemokine C-X-C motif ligand 2 (CXCL2)
    Authors: Xian K Zhang
    Mol. Immunol., 2016;81(0):59-66.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  19. Poly-N-acetyl glucosamine production by Staphylococcus epidermidis cells increases their in vivo pro-inflammatory effect
    Infect Immun, 2016;0(0):.
    Species: Mouse
    Sample Types: Tissue Secretion
  20. The Fli-1 transcription factor is a critical regulator for controlling the expression of chemokine C-X-C motif ligand 2 (CXCL2)
    Mol. Immunol., 2016;81(0):59-66.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  21. Plasminogen activator inhibitor-1 stimulates macrophage activation through Toll-like Receptor-4
    Authors: Kamlesh K Gupta
    Biochem Biophys Res Commun, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  22. Host Inflammatory Response to Mosquito Bites Enhances the Severity of Arbovirus Infection
    Authors: Marieke Pingen
    Immunity, 2016;44(6):1455-69.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Klebsiella pneumoniae Siderophores Induce Inflammation, Bacterial Dissemination, and HIF-1? Stabilization during Pneumonia
    MBio, 2016;7(5):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  24. Palmitate promotes inflammatory responses and cellular senescence in cardiac fibroblasts
    Authors: Arne Yndestad
    Biochim. Biophys. Acta, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. TRAIL signaling is pro-inflammatory and pro-viral in a murine model of rhinovirus 1B infection
    Authors: Joerg Mattes
    Am. J. Physiol. Lung Cell Mol. Physiol., 2016;0(0):ajplung.00200.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Mast cells play an important role in chlamydia pneumoniae lung infection by facilitating immune cell recruitment into the airway.
    Authors: Chiba N, Shimada K, Chen S, Jones H, Alsabeh R, Slepenkin A, Peterson E, Crother T, Arditi M
    J Immunol, 2015;194(8):3840-51.
    Species: Mouse
    Sample Types: BALF
  27. C1q Deficiency Promotes Pulmonary Vascular Inflammation and Enhances the Susceptibility of the Lung Endothelium to Injury.
    Authors: Shah D, Romero F, Zhu Y, Duong M, Sun J, Walsh K, Summer R
    J Biol Chem, 2015;290(49):29642-51.
    Species: Mouse
    Sample Types: BALF
  28. Influenza A virus-dependent remodeling of pulmonary clock function in a mouse model of COPD.
    Authors: Sundar, Isaac K, Ahmad, Tanveer, Yao, Hongwei, Hwang, Jae-woon, Gerloff, Janice, Lawrence, B Paige, Sellix, Michael, Rahman, Irfan
    Sci Rep, 2015;4(0):9927.
    Species: Mouse
    Sample Types: BALF
  29. Necroptosis suppresses inflammation via termination of TNF- or LPS-induced cytokine and chemokine production.
    Authors: Kearney C, Cullen S, Tynan G, Henry C, Clancy D, Lavelle E, Martin S
    Cell Death Differ, 2015;22(8):1313-27.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. C-Type Lectin Receptor MCL Facilitates Mincle Expression and Signaling through Complex Formation.
    Authors: Miyake Y, Masatsugu O, Yamasaki S
    J Immunol, 2015;194(11):5366-74.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  31. A critical role for the TLR signaling adapter Mal in alveolar macrophage-mediated protection against Bordetella pertussis.
    Authors: Bernard N, Finlay C, Tannahill G, Cassidy J, O'Neill L, Mills K
    Mucosal Immunol, 2015;8(5):982-92.
    Species: Mouse
    Sample Types: Tissue Homogenates
  32. The transcription factor ATF7 mediates lipopolysaccharide-induced epigenetic changes in macrophages involved in innate immunological memory.
    Authors: Yoshida K, Maekawa T, Zhu Y, Renard-Guillet C, Chatton B, Inoue K, Uchiyama T, Ishibashi K, Yamada T, Ohno N, Shirahige K, Okada-Hatakeyama M, Ishii S
    Nat Immunol, 2015;16(10):1034-43.
    Species: Mouse
    Sample Types: Serum
  33. Gene and protein expression in response to different growth temperatures and oxygen availability in Burkholderia thailandensis.
    Authors: Peano C, Chiaramonte F, Motta S, Pietrelli A, Jaillon S, Rossi E, Consolandi C, Champion O, Michell S, Freddi L, Falciola L, Basilico F, Garlanda C, Mauri P, De Bellis G, Landini P
    PLoS ONE, 2014;9(3):e93009.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  34. Role of leukotriene A4 hydrolase aminopeptidase in the pathogenesis of emphysema.
    Authors: Paige M, Wang K, Burdick M, Park S, Cha J, Jeffery E, Sherman N, Shim Y
    J Immunol, 2014;192(11):5059-68.
    Species: Mouse
    Sample Types: BALF
  35. Toll-like receptor 9 mediated responses in cardiac fibroblasts.
    Authors: Ohm I, Alfsnes K, Belland Olsen M, Ranheim T, Sandanger O, Dahl T, Aukrust P, Finsen A, Yndestad A, Vinge L
    PLoS ONE, 2014;9(8):e104398.
  36. No Impairment in host defense against Streptococcus pneumoniae in obese CPEfat/fat mice.
    Authors: Mancuso P, O Brien E, Prano J, Goel D, Aronoff D
    PLoS ONE, 2014;9(9):e106420.
    Species: Mouse
    Sample Types: BALF
  37. Signaling via tumor necrosis factor receptor 1 but not Toll-like receptor 2 contributes significantly to hydrosalpinx development following Chlamydia muridarum infection.
    Authors: Dong X, Liu Y, Chang X, Lei L, Zhong G
    Infect Immun, 2014;82(5):1833-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Chinese herbal medicine suppresses invasion-promoting capacity of cancer-associated fibroblasts in pancreatic cancer.
    Authors: Chen, Lianyu, Qu, Chao, Chen, Hao, Xu, Litao, Qi, Qi, Luo, Jianmin, Wang, Kun, Meng, Zhiqiang, Chen, Zhen, Wang, Peng, Liu, Luming
    PLoS ONE, 2014;9(4):e96177.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Hyaluronan digestion controls DC migration from the skin.
    Authors: Muto, Jun, Morioka, Yasuhide, Yamasaki, Kenshi, Kim, Margaret, Garcia, Andrea, Carlin, Aaron F, Varki, Ajit, Gallo, Richard
    J Clin Invest, 2014;124(3):1309-19.
    Species: Mouse
    Sample Types: Tissue Homogenates
  40. The lung inflammation and skeletal muscle wasting induced by subchronic cigarette smoke exposure are not altered by a high-fat diet in mice.
    Authors: Hansen, Michelle, Chen, Hui, Jones, Jessica, Langenbach, Shenna Y, Vlahos, Ross, Gualano, Rosa C, Morris, Margaret, Anderson, Gary P
    PLoS ONE, 2013;8(11):e80471.
    Species: Mouse
    Sample Types: Tissue Homogenates
  41. Genetic deletion of the HIF-1alpha isoform I.1 in T cells enhances antibacterial immunity and improves survival in a murine peritonitis model.
    Authors: Georgiev P, Belikoff B, Hatfield S, Ohta A, Sitkovsky M, Lukashev D
    Eur J Immunol, 2013;43(3):655-66.
    Species: Mouse
    Sample Types: Serum
  42. Myeloid cell sirtuin-1 expression does not alter host immune responses to Gram-negative endotoxemia or Gram-positive bacterial infection.
    Authors: Crotty Alexander, Laura E, Marsh, Brenda J, Timmer, Anjuli M, Lin, Ann E, Zainabadi, Kayvan, Czopik, Agnieszk, Guarente, Leonard, Nizet, Victor
    PLoS ONE, 2013;8(12):e84481.
    Species: Mouse
    Sample Types: Plasma
  43. The novel cytokine interleukin-33 activates acinar cell proinflammatory pathways and induces acute pancreatic inflammation in mice.
    Authors: Kempuraj D, Twait E, Williard D, Yuan Z, Meyerholz D, Samuel I
    PLoS ONE, 2013;8(2):e56866.
    Species: Mouse
    Sample Types: Tissue Homogenates
  44. Milano summer particulate matter (PM10) triggers lung inflammation and extra pulmonary adverse events in mice.
    Authors: Farina F, Sancini G, Battaglia C, Tinaglia V, Mantecca P, Camatini M, Palestini P
    PLoS ONE, 2013;8(2):e56636.
    Species: Mouse
    Sample Types: BALF
  45. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012;7(2):e32125.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  46. Milk fat globule-epidermal growth factor-factor 8 attenuates neutrophil infiltration in acute lung injury via modulation of CXCR2.
    Authors: Aziz M, Matsuda A, Yang WL, Jacob A, Wang P
    J. Immunol., 2012;189(1):393-402.
    Species: Mouse
    Sample Types: Tissue Homogenates
  47. Role of Toll interleukin-1 receptor (IL-1R) 8, a negative regulator of IL-1R/Toll-like receptor signaling, in resistance to acute Pseudomonas aeruginosa lung infection.
    Authors: Veliz Rodriguez T, Moalli F, Polentarutti N, Paroni M, Bonavita E, Anselmo A, Nebuloni M, Mantero S, Jaillon S, Bragonzi A, Mantovani A, Riva F, Garlanda C
    Infect. Immun., 2012;80(1):100-9.
    Species: Mouse
    Sample Types: Serum
  48. Altered regulation of Toll-like receptor responses impairs antibacterial immunity in the allergic lung.
    Authors: Habibzay, M, Saldana, J I, Goulding, J, Lloyd, C M, Hussell, T
    Mucosal Immunol, 2012;5(5):524-34.
    Species: Mouse
    Sample Types: BALF
  49. Fecal lipocalin 2, a sensitive and broadly dynamic non-invasive biomarker for intestinal inflammation.
    Authors: Chassaing B, Srinivasan G, Delgado M, Young A, Gewirtz A, Vijay-Kumar M
    PLoS ONE, 2012;7(9):e44328.
    Species: Mouse
    Sample Types: Serum
  50. Lung-targeted overexpression of the NF-kappaB member RelB inhibits cigarette smoke-induced inflammation.
    Authors: McMillan DH, Baglole CJ, Thatcher TH, Maggirwar S, Sime PJ, Phipps RP
    Am. J. Pathol., 2011;179(1):125-33.
    Species: Mouse
    Sample Types: BALF
  51. Recombinant human tissue factor pathway inhibitor exerts anticoagulant, anti-inflammatory and antimicrobial effects in murine pneumococcal pneumonia.
    Authors: Van Den Boogaard FE, Brands X, Schultz MJ, Levi M, Roelofs JJ, Van 't Veer C, van der Poll T
    J. Thromb. Haemost., 2011;9(1):122-32.
  52. Leukocyte ADAM17 Regulates Acute Pulmonary Inflammation.
    Authors: Arndt PG, Strahan B, Wang Y, Long C, Horiuchi K, Walcheck B
    PLoS ONE, 2011;6(5):e19938.
    Species: Mouse
    Sample Types: BALF
  53. The Therapeutic Potential of the Humoral Pattern Recognition Molecule PTX3 in Chronic Lung Infection Caused by Pseudomonas aeruginosa.
    Authors: Moalli F, Paroni M, Veliz Rodriguez T, Riva F, Polentarutti N, Bottazzi B, Valentino S, Mantero S, Nebuloni M, Mantovani A, Bragonzi A, Garlanda C
    J. Immunol., 2011;186(9):5425-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  54. Phosphoinositide 3-kinase gamma plays a critical role in bleomycin-induced pulmonary inflammation and fibrosis in mice.
    Authors: Russo RC, Garcia CC, Barcelos LS, Rachid MA, Guabiraba R, Roffe E, Souza AL, Sousa LP, Mirolo M, Doni A, Cassali GD, Pinho V, Locati M, Teixeira MM
    J. Leukoc. Biol., 2011;89(2):269-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
  55. Local delivery of GM-CSF protects mice from lethal pneumococcal pneumonia.
    Authors: Steinwede K, Tempelhof O, Bolte K, Maus R, Bohling J, Ueberberg B, Langer F, Christman JW, Paton JC, Ask K, Maharaj S, Kolb M, Gauldie J, Welte T, Maus UA
    J. Immunol., 2011;187(10):5346-56.
    Species: Mouse
    Sample Types: BALF
  56. Inflammatory chemokine release of astrocytes in vitro is reduced by all-trans retinoic acid.
    Authors: van Neerven S, Regen T, Wolf D, Nemes A, Johann S, Beyer C, Hanisch UK, Mey J
    J. Neurochem., 2010;114(5):1511-26.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  57. Distinct roles of hepatocyte- and myeloid cell-derived IL-1 receptor antagonist during endotoxemia and sterile inflammation in mice.
    Authors: Lamacchia C, Palmer G, Bischoff L, Rodriguez E, Talabot-Ayer D, Gabay C
    J. Immunol., 2010;185(0):2516-24.
    Species: Mouse
    Sample Types: Serum
  58. Ligands of the receptor for advanced glycation end products, including high-mobility group box 1, limit bacterial dissemination during Escherichia coli peritonitis.
    Authors: van Zoelen MA, Achouiti A, Schmidt AM, Yang H, Florquin S, Tracey KJ, van der Poll T
    Crit. Care Med., 2010;38(6):1414-22.
    Species: Mouse
    Sample Types: Peritoneal Fluid
  59. Impact of adiponectin deficiency on pulmonary responses to acute ozone exposure in mice.
    Authors: Zhu M, Hug C, Kasahara DI
    Am. J. Respir. Cell Mol. Biol., 2010;43(4):487-97.
    Species: Mouse
    Sample Types: BALF
  60. Role of endothelial TLR4 for neutrophil recruitment into central nervous system microvessels in systemic inflammation.
    Authors: Zhou H, Andonegui G, Wong CH, Kubes P
    J. Immunol., 2009;183(8):5244-50.
    Species: Mouse
    Sample Types: Plasma
  61. Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
    Authors: van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
    Clin. Exp. Immunol., 2009;158(1):143-53.
    Species: Mouse
    Sample Types: Tissue Homogenates
  62. TREM-1 activation alters the dynamics of pulmonary IRAK-M expression in vivo and improves host defense during pneumococcal pneumonia.
    Authors: Lagler H, Sharif O, Haslinger I, Matt U, Stich K, Furtner T, Doninger B, Schmid K, Gattringer R, de Vos AF, Knapp S
    J. Immunol., 2009;183(3):2027-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
  63. Scavenging roles of chemokine receptors: chemokine receptor deficiency is associated with increased levels of ligand in circulation and tissues.
    Authors: Cardona AE, Sasse ME, Liu L, Cardona SM, Mizutani M, Savarin C, Hu T, Ransohoff RM
    Blood, 2008;112(2):256-63.
    Species: Mouse
    Sample Types: Tissue Homogenates
  64. Neurokinin A engages neurokinin-1 receptor to induce NF-kappaB-dependent gene expression in murine macrophages: implications of ERK1/2 and PI 3-kinase/Akt pathways.
    Authors: Sun J, Ramnath RD, Tamizhselvi R, Bhatia M
    Am. J. Physiol., Cell Physiol., 2008;295(3):C679-91.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  65. Expression of inflammatory mediators in the otitis media induced by Helicobacter pylori antigen in mice.
    Authors: Kariya S, Okano M, Fukushima K, Nomiya S, Kataoka Y, Nomiya R, Akagi H, Nishizaki K
    Clin. Exp. Immunol., 2008;154(1):134-40.
    Species: Mouse
    Sample Types: Middle Ear Lavage Fluid
  66. Administration of a synthetic TLR4 agonist protects mice from pneumonic tularemia.
    Authors: Lembo A, Pelletier M, Iyer R, Timko M, Dudda JC, West TE, Wilson CB, Hajjar AM, Skerrett SJ
    J. Immunol., 2008;180(11):7574-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
  67. Surfactant protein D alters allergic lung responses in mice and human subjects.
    Authors: Brandt EB, Mingler MK, Stevenson MD, Wang N, Khurana Hershey GK, Whitsett JA, Rothenberg ME
    J. Allergy Clin. Immunol., 2008;121(5):1140-1147.e2.
    Species: Mouse
    Sample Types: BALF
  68. An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.
    Authors: Nomellini V, Faunce DE, Gomez CR, Kovacs EJ
    J. Leukoc. Biol., 2008;83(6):1493-501.
  69. The chemokine receptors CXCR1/CXCR2 modulate antigen-induced arthritis by regulating adhesion of neutrophils to the synovial microvasculature.
    Authors: Coelho FM, Pinho V, Amaral FA, Sachs D, Costa VV, Rodrigues DH, Vieira AT, Silva TA, Souza DG, Bertini R, Teixeira AL, Teixeira MM
    Arthritis Rheum., 2008;58(8):2329-37.
    Species: Mouse
    Sample Types: Tissue Homogenates
  70. Role of mesothelial cell-derived granulocyte colony-stimulating factor in interleukin-17-induced neutrophil accumulation in the peritoneum.
    Authors: Witowski J, Ksiazek K, Warnecke C, Kuzlan M, Korybalska K, Tayama H, Wisniewska-Elnur J, Pawlaczyk K, Trominska J, Breborowicz A, Jorres A
    Kidney Int., 2007;71(6):514-25.
    Species: Mouse
    Sample Types: Peritoneal Lavage
  71. Neurokinin-1 receptor antagonist treatment protects mice against lung injury in polymicrobial sepsis.
    Authors: Hegde A, Zhang H, Moochhala SM, Bhatia M
    J. Leukoc. Biol., 2007;82(3):678-85.
    Species: Mouse
    Sample Types: Plasma
  72. Involvement of vitronectin in lipopolysaccaride-induced acute lung injury.
    Authors: Tsuruta Y, Park YJ, Siegal GP, Liu G, Abraham E
    J. Immunol., 2007;179(10):7079-86.
    Species: Mouse
    Sample Types: Tissue Homogenates
  73. Nod1-mediated innate immune recognition of peptidoglycan contributes to the onset of adaptive immunity.
    Authors: Fritz JH, Le Bourhis L, Sellge G, Magalhaes JG, Fsihi H, Kufer TA, Collins C, Viala J, Ferrero RL, Girardin SE, Philpott DJ
    Immunity, 2007;26(4):445-59.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  74. Caspase-1 contributes to Chlamydia trachomatis-induced upper urogenital tract inflammatory pathologies without affecting the course of infection.
    Authors: Cheng W, Shivshankar P, Li Z, Chen L, Yeh IT, Zhong G
    Infect. Immun., 2007;76(2):515-22.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  75. Minocycline modulates neuroinflammation independently of its antimicrobial activity in staphylococcus aureus-induced brain abscess.
    Authors: Kielian T, Esen N, Liu S, Phulwani NK, Syed MM, Phillips N, Nishina K, Cheung AL, Schwartzman JD, Ruhe JJ
    Am. J. Pathol., 2007;171(4):1199-214.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  76. Role of Toll-like receptor 2 in recognition of Legionella pneumophila in a murine pneumonia model.
    Authors: Fuse ET, Tateda K, Kikuchi Y, Matsumoto T, Gondaira F, Azuma A, Kudoh S, Standiford TJ, Yamaguchi K
    J. Med. Microbiol., 2007;56(0):305-12.
    Species: Mouse
    Sample Types: Tissue Homogenates
  77. Differential involvement of TLR2 and TLR4 in host survival during pulmonary infection with Chlamydia pneumoniae.
    Authors: Rodriguez N, Wantia N, Fend F, Durr S, Wagner H, Miethke T
    Eur. J. Immunol., 2006;36(5):1145-55.
    Species: Mouse
    Sample Types: Tissue Homogenates
  78. Toll-like receptor 4 signaling leads to neutrophil migration impairment in polymicrobial sepsis.
    Authors: Alves-Filho JC, de Freitas A, Russo M, Cunha FQ
    Crit. Care Med., 2006;34(2):461-70.
    Species: Mouse
    Sample Types: Tissue Homogenates
  79. Effects of dexamethazone on LPS-induced activationand migration of mouse dendritic cells revealed by a genome-wide transcriptional analysis.
    Authors: Vizzardelli C, Pavelka N, Luchini A, Zanoni I, Bendickson L, Pelizzola M, Beretta O, Foti M, Granucci F, Nilsen-Hamilton M, Ricciardi-Castagnoli P
    Eur. J. Immunol., 2006;36(6):1504-15.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  80. TLR4 mediates vaccine-induced protective cellular immunity to Bordetella pertussis: role of IL-17-producing T cells.
    Authors: Higgins SC, Jarnicki AG, Lavelle EC, Mills KH
    J. Immunol., 2006;177(11):7980-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  81. Increased pulmonary responses to acute ozone exposure in obese db/db mice.
    Authors: Lu FL, Johnston RA, Flynt L, Theman TA, Terry RD, Schwartzman IN, Lee A, Shore SA
    Am. J. Physiol. Lung Cell Mol. Physiol., 2006;290(5):L856-65.
    Species: Mouse
    Sample Types: Serum
  82. Mucosal administration of CpG oligodeoxynucleotide elicits strong CC and CXC chemokine responses in the vagina and serves as a potent Th1-tilting adjuvant for recombinant gD2 protein vaccination against genital herpes.
    Authors: Tengvall S, Lundqvist A, Eisenberg RJ, Cohen GH, Harandi AM
    J. Virol., 2006;80(11):5283-91.
    Species: Mouse
    Sample Types: Tissue Homogenates
  83. The pulmonary and hepatic immune microenvironment and its contribution to the early systemic inflammation following blunt chest trauma.
    Authors: Perl M, Gebhard F, Braumuller S, Tauchmann B, Bruckner UB, Kinzl L, Knoferl MW
    Crit. Care Med., 2006;34(4):1152-9.
    Species: Mouse
    Sample Types: BALF
  84. Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice.
    Authors: Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK
    Am. J. Respir. Cell Mol. Biol., 2005;33(3):271-9.
    Species: Mouse
    Sample Types: BALF
  85. An essential role for non-bone marrow-derived cells in control of Pseudomonas aeruginosa pneumonia.
    Authors: Hajjar AM, Harowicz H, Liggitt HD, Fink PJ, Wilson CB, Skerrett SJ
    Am. J. Respir. Cell Mol. Biol., 2005;33(5):470-5.
    Species: Mouse
    Sample Types: Tissue Homogenates
  86. Pyocyanin production by Pseudomonas aeruginosa induces neutrophil apoptosis and impairs neutrophil-mediated host defenses in vivo.
    Authors: Allen L, Dockrell DH, Pattery T, Lee DG, Cornelis P, Hellewell PG, Whyte MK
    J. Immunol., 2005;174(6):3643-9.
    Species: Mouse
    Sample Types: BALF
  87. Toll-like receptor 2 (TLR2) mediates astrocyte activation in response to the Gram-positive bacterium Staphylococcus aureus.
    Authors: Esen N, Tanga FY, DeLeo JA, Kielian T
    J. Neurochem., 2004;88(3):746-58.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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Reviews for Mouse CXCL2/MIP-2 DuoSet ELISA

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Mouse CXCL2/MIP-2 DuoSet ELISA
By Anonymous on 03/28/2018
Sample Tested: balb/c mouse

Mouse CXCL2/MIP-2 DuoSet ELISA
By Anonymous on 10/16/2016
Sample Tested: Cell culture supernatant

Really simple! But the standard concentrations are not high enough. All my supernatants were above the highest limit of detection after dilutions.


Mouse CXCL2/MIP-2 DuoSet ELISA
By Anonymous on 10/06/2016
Sample Tested: Cell culture supernatant

Mouse CXCL2/MIP-2 DuoSet ELISA
By Anonymous on 06/03/2016
Sample Tested: Blood Serum,Cell culture supernatant