Mouse CXCL9/MIG Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
MCX900
CXCL9/MIG ELISA Kit ELISA
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Product Details
Procedure
Citations (22)
FAQs
Supplemental Products
Reviews (2)

Mouse CXCL9/MIG Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
Sensitivity
7.8 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIG in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIG and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIG.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 23 23 23
Mean 77 216 815 66 190 741
Standard Deviation 6.2 14.8 68 5.5 14.1 39.5
CV% 8.1 6.9 8.3 8.3 7.4 5.3

Recovery

The recovery of mouse MIG spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 95 81-116
EDTA Plasma (n=9) 94 81-107
Serum (n=9) 93 85-112
Tissue Homogenates (n=2) 104 88-118

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MIG in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CXCL9/MIG ELISA Linearity

Data Examples

Mouse CXCL9/MIG ELISA Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CXCL9/MIG

CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.

Entrez Gene IDs:
4283 (Human); 17329 (Mouse); 246759 (Rat)
Alternate Names:
chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse CXCL9/MIG Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Murine ?-Herpesvirus 68 Induces Severe Lung Inflammation in IL-27-Deficient Mice with Liver Dysfunction Preventable by Oral Neomycin
    Authors: K Kanai, AM Park, A Watanabe, T Arikawa, T Yasui, H Yoshida, I Tsunoda, O Yoshie
    J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: BALF
  2. CXCR3/CXCL10 Axis Shapes Tissue Distribution of Memory Phenotype CD8+ T Cells in Nonimmunized Mice
    Authors: C Alanio, R Barreira d, D Michonneau, P Bousso, MA Ingersoll, ML Albert
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. CXCL9 compensates for the absence of CXCL10 during recurrent Herpetic stromal keratitis
    Authors: D Tajfirouz, DM West, XT Yin, CA Potter, R Klein, PM Stuart
    Virology, 2017;506(0):7-13.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer's disease model.
    Authors: Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M
    J Clin Invest, 2015;125(1):365-78.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  6. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
    Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
    J Immunol, 2014;192(4):1671-80.
    Species: Mouse
    Sample Types: Cell Lysates
  7. Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with
    Authors: Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y
    Oncoimmunology, 2013;2(8):e25752.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
    Authors: Gottfries J, Melgar S, Michaelsson E
    PLoS ONE, 2012;7(1):e30005.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. NKT cells mediate pulmonary inflammation and dysfunction in murine sickle cell disease through production of IFN-gamma and CXCR3 chemokines.
    Authors: Wallace KL, Marshall MA, Ramos SI, Lannigan JA, Field JJ, Strieter RM, Linden J
    Blood, 2009;114(3):667-76.
    Species: Mouse
    Sample Types: Tissue Homogenates
  10. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
    Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
    Am. J. Pathol., 2008;172(5):1209-21.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria.
    Authors: Campanella GS, Tager AM, El Khoury JK, Thomas SY, Abrazinski TA, Manice LA, Colvin RA, Luster AD
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(12):4814-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
  12. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
    Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
    Am. J. Pathol., 2007;170(4):1152-64.
    Species: Mouse
    Sample Types: Tissue Homogenates
  13. TLR3 ligand stimulates fully functional memory CD8+ T cells in the absence of CD4+ T-cell help.
    Authors: Hervas-Stubbs S, Olivier A, Boisgerault F, Thieblemont N, Leclerc C
    Blood, 2007;109(12):5318-26.
    Species: Mouse
    Sample Types: Serum
  14. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis.
    Authors: Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B
    Infect. Immun., 2006;74(6):3618-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
  16. CXCL10 is the key ligand for CXCR3 on CD8+ effector T cells involved in immune surveillance of the lymphocytic choriomeningitis virus-infected central nervous system.
    Authors: Christensen JE, de Lemos C, Moos T, Christensen JP, Thomsen AR
    J. Immunol., 2006;176(7):4235-43.
    Species: Mouse
    Sample Types: CSF
  17. Persistent effects induced by IL-13 in the lung.
    Authors: Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME
    Am. J. Respir. Cell Mol. Biol., 2006;35(3):337-46.
    Species: Mouse
    Sample Types: BALF
  18. Intact TRL 9 and type I interferon signaling pathways are required to augment HSV-1 induced corneal CXCL9 and CXCL10.
    Authors: Wuest T, Austin BA, Uematsu S, Thapa M, Akira S, Carr DJ
    J. Neuroimmunol., 2006;179(1):46-52.
    Species: Mouse
    Sample Types: Tissue Homogenates
  19. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
    Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
    Blood, 2005;107(5):2112-22.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  20. The human herpes virus 8-encoded chemokine receptor is required for angioproliferation in a murine model of Kaposi's sarcoma.
    Authors: Jensen KK, Manfra DJ, Grisotto MG, Martin AP, Vassileva G, Kelley K, Schwartz TW, Lira SA
    J. Immunol., 2005;174(6):3686-94.
    Species: Mouse
    Sample Types: Tissue Homogenates
  21. Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
    Authors: Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC
    Blood, 2004;104(12):3789-96.
    Species: Mouse
    Sample Types: Tissue Homogenates
  22. Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts.
    Authors: Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC
    Circulation, 2004;110(11):II194-9.
    Species: Mouse
    Sample Types: Tissue Homogenates

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Reviews for Mouse CXCL9/MIG Quantikine ELISA Kit

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Mouse CXCL9/MIG Quantikine ELISA Kit
By Anonymous on 11/11/2018
Sample Tested: mouse mammary tumor

Produced results with expected direction according to mRNA and functional assays, reasonably easy to perform.


Mouse CXCL9/MIG Quantikine ELISA Kit
By Anonymous on 05/02/2017
Sample Tested: Lung tissue