Mouse CXCL9/MIG Quantikine ELISA Kit

(19 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Tissue Homogenates (50 uL), Serum (25 uL), EDTA Plasma (25 uL)
  • Sensitivity
    7.8 pg/mL
  • Assay Range
    31.2 - 2,000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma)
  • Specificity
    Natural and recombinant mouse MIG
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIG in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIG and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIG.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation6.214.8685.514.139.5


The recovery of mouse MIG spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 95 81-116
EDTA Plasma (n=9) 94 81-107
Serum (n=9) 93 85-112
Tissue Homogenates (n=2) 104 88-118
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MIG in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse CXCL9/MIG Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL9/MIG
MIG, a member of the CXC subfamily of chemokines that lack the ELR domain, was initially identified as a lymphokine-activated gene in mouse macrophages. MIG is a chemoattractant for activated T cells and TIL, but not for neutrophils or monocytes.
    • Entrez Gene IDs
      4283 (Human); 17329 (Mouse); 246759 (Rat);
    • Alternate Names
      chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 19
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    Sample Type
    1. CXCR3 promotes plaque formation and behavioral deficits in an Alzheimer's disease model.
      Authors: Krauthausen M, Kummer M, Zimmermann J, Reyes-Irisarri E, Terwel D, Bulic B, Heneka M, Muller M
      J Clin Invest, 2015;125(1):365-78.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. Cleavage of the T cell protein tyrosine phosphatase by the hepatitis C virus nonstructural 3/4A protease induces a Th1 to Th2 shift reversible by ribavirin therapy.
      Authors: Brenndorfer E, Brass A, Karthe J, Ahlen G, Bode J, Sallberg M
      J Immunol, 2014;192(4):1671-80.
      Species: Mouse
      Sample Type: Cell Extracts
    3. Interferon gamma-induced intratumoral expression of CXCL9 alters the local distribution of T cells following immunotherapy with
      Authors: Guirnalda P, Wood L, Goenka R, Crespo J, Paterson Y
      Oncoimmunology, 2013;2(8):e25752.
      Species: Mouse
      Sample Type: Homogenized Tissue
    4. Modelling of mouse experimental colitis by global property screens: a holistic approach to assess drug effects in inflammatory bowel disease.
      Authors: Gottfries J, Melgar S, Michaelsson E
      PLoS ONE, 2012;7(1):e30005.
      Species: Mouse
      Sample Type: Tissue Homogenates
    5. NKT cells mediate pulmonary inflammation and dysfunction in murine sickle cell disease through production of IFN-gamma and CXCR3 chemokines.
      Authors: Wallace KL, Marshall MA, Ramos SI, Lannigan JA, Field JJ, Strieter RM, Linden J
      Blood, 2009;114(3):667-76.
      Species: Mouse
      Sample Type: Tissue Homogenates
    6. Chemokine receptor CXCR3 and its ligands CXCL9 and CXCL10 are required for the development of murine cerebral malaria.
      Authors: Campanella GS, Tager AM, El Khoury JK, Thomas SY, Abrazinski TA, Manice LA, Colvin RA, Luster AD
      Proc. Natl. Acad. Sci. U.S.A., 2008;105(12):4814-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    7. A novel mechanism for CCR4 in the regulation of macrophage activation in bleomycin-induced pulmonary fibrosis.
      Authors: Trujillo G, O'Connor EC, Kunkel SL, Hogaboam CM
      Am. J. Pathol., 2008;172(5):1209-21.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. TLR3 ligand stimulates fully functional memory CD8+ T cells in the absence of CD4+ T-cell help.
      Authors: Hervas-Stubbs S, Olivier A, Boisgerault F, Thieblemont N, Leclerc C
      Blood, 2007;109(12):5318-26.
      Species: Mouse
      Sample Type: Serum
    9. Therapeutic targeting of CC ligand 21 or CC chemokine receptor 7 abrogates pulmonary fibrosis induced by the adoptive transfer of human pulmonary fibroblasts to immunodeficient mice.
      Authors: Pierce EM, Carpenter K, Jakubzick C, Kunkel SL, Flaherty KR, Martinez FJ, Hogaboam CM
      Am. J. Pathol., 2007;170(4):1152-64.
      Species: Mouse
      Sample Type: Tissue Homogenates
    10. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
      Authors: Fujita H, Asahina A, Komine M, Tamaki K
      Cell. Immunol., 2007;245(2):70-9.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    11. Intact TRL 9 and type I interferon signaling pathways are required to augment HSV-1 induced corneal CXCL9 and CXCL10.
      Authors: Wuest T, Austin BA, Uematsu S, Thapa M, Akira S, Carr DJ
      J. Neuroimmunol., 2006;179(1):46-52.
      Species: Mouse
      Sample Type: Tissue Homogenates
    12. CXCL10 is the key ligand for CXCR3 on CD8+ effector T cells involved in immune surveillance of the lymphocytic choriomeningitis virus-infected central nervous system.
      Authors: Christensen JE, de Lemos C, Moos T, Christensen JP, Thomsen AR
      J. Immunol., 2006;176(7):4235-43.
      Species: Mouse
      Sample Type: CSF
    13. Organ-specific role of MyD88 for gene regulation during polymicrobial peritonitis.
      Authors: Weighardt H, Mages J, Jusek G, Kaiser-Moore S, Lang R, Holzmann B
      Infect. Immun., 2006;74(6):3618-32.
      Species: Mouse
      Sample Type: Tissue Homogenates
    14. Persistent effects induced by IL-13 in the lung.
      Authors: Fulkerson PC, Fischetti CA, Hassman LM, Nikolaidis NM, Rothenberg ME
      Am. J. Respir. Cell Mol. Biol., 2006;35(3):337-46.
      Species: Mouse
      Sample Type: BALF
    15. A distinct and unique transcriptional program expressed by tumor-associated macrophages (defective NF-kappaB and enhanced IRF-3/STAT1 activation).
      Authors: Biswas SK, Gangi L, Paul S, Schioppa T, Saccani A, Sironi M, Bottazzi B, Doni A, Vincenzo B, Pasqualini F, Vago L, Nebuloni M, Mantovani A, Sica A
      Blood, 2005;107(5):2112-22.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    16. The human herpes virus 8-encoded chemokine receptor is required for angioproliferation in a murine model of Kaposi's sarcoma.
      Authors: Jensen KK, Manfra DJ, Grisotto MG, Martin AP, Vassileva G, Kelley K, Schwartz TW, Lira SA
      J. Immunol., 2005;174(6):3686-94.
      Species: Mouse
      Sample Type: Tissue Homogenates
    17. Suppression of graft coronary artery disease by a brief treatment with a selective epsilonPKC activator and a deltaPKC inhibitor in murine cardiac allografts.
      Authors: Tanaka M, Terry RD, Mokhtari GK, Inagaki K, Koyanagi T, Kofidis T, Mochly-Rosen D, Robbins RC
      Circulation, 2004;110(11):II194-9.
      Species: Mouse
      Sample Type: Tissue Homogenates
    18. Blockade of CXCR3 receptor:ligand interactions reduces leukocyte recruitment to the lung and the severity of experimental idiopathic pneumonia syndrome.
      Authors: Hildebrandt GC, Corrion LA, Olkiewicz KM, Lu B, Lowler K, Duffner UA, Moore BB, Kuziel WA, Liu C, Cooke KR
      J. Immunol., 2004;173(3):2050-9.
      Species: Mouse
      Sample Type: Serum
    19. Cardiomyocyte-specific Bcl-2 overexpression attenuates ischemia-reperfusion injury, immune response during acute rejection, and graft coronary artery disease.
      Authors: Tanaka M, Nakae S, Terry RD, Mokhtari GK, Gunawan F, Balsam LB, Kaneda H, Kofidis T, Tsao PS, Robbins RC
      Blood, 2004;104(12):3789-96.
      Species: Mouse
      Sample Type: Tissue Homogenates
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