CXCL9, also known as MIG, is a chemokine that is induced in macrophages and in CNS glial cells in response to IFN-gamma. CXCL9 signals through CXCR3 to induce the chemoattraction of activated T cells and tumor-infiltrating lymphocytes.
Mouse CXCL9/MIG Quantikine ELISA Kit
R&D Systems | Catalog # MCX900
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Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL)
Sensitivity
7.8 pg/mL
Assay Range
31.2-2000 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma)
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Product Summary for Mouse CXCL9/MIG Quantikine ELISA Kit
The Quantikine Mouse MIG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse MIG in cell culture supernates, tissue homogenates, serum, and plasma. It contains E. coli-expressed recombinant mouse MIG and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse MIG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse MIG.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Mouse
Specificity
Natural and recombinant mouse MIG
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 23 | 23 | 23 |
| Mean (pg/mL) | 77 | 216 | 815 | 66 | 190 | 741 |
| Standard Deviation | 6.2 | 14.8 | 68 | 5.5 | 14.1 | 39.5 |
| CV% | 8.1 | 6.9 | 8.3 | 8.3 | 7.4 | 5.3 |
Recovery for Mouse CXCL9/MIG Quantikine ELISA Kit
The recovery of mouse MIG spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 95 | 81-116 |
| EDTA Plasma (n=9) | 94 | 81-107 |
| Serum (n=9) | 93 | 85-112 |
| Tissue Homogenates (n=2) | 104 | 88-118 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse MIG in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse CXCL9/MIG Quantikine ELISA Kit
Mouse CXCL9/MIG ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL9/MIG
Additional CXCL9/MIG Products
Product Documents for Mouse CXCL9/MIG Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CXCL9/MIG Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Mouse CXCL9/MIG Quantikine ELISA Kit
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Sample Tested: colon and Colon tissueVerified Customer | Posted 07/05/2023
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Sample Tested: HT-29 human colon adenocarcinoma cell lineVerified Customer | Posted 02/12/2020
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Sample Tested: 4T1 mouse breast cancer cell lineVerified Customer | Posted 02/03/2020
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Sample Tested: mouse mammary tumorVerified Customer | Posted 11/11/2018Produced results with expected direction according to mRNA and functional assays, reasonably easy to perform.
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Sample Tested: Lung tissueVerified Customer | Posted 05/02/2017
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Protocols
View specific protocols for Mouse CXCL9/MIG Quantikine ELISA Kit (MCX900):
Refer to the product for complete assay procedure.
Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
50 µL Assay Diluent
50 µL Standard, Control, or Sample
100 µL Conjugate
100 µL Substrate Solution
100 µL Stop Solution
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse CXCL9/MIG Quantikine ELISA Kit
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Q: Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?
A: A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.
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Associated Pathways
