Mouse CXCR2/IL-8 RB PE-conjugated Antibody

(20 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Stains mouse CXCR2-transfected cells but not CXCR1 transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 242216
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C6 rat glioma cell line transfected with mouse CXCR2
    Met1-Leu359
    Accession # P35343
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CXCR2/IL‑8 RB in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr‑1/Ly‑6G APC‑conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat Anti-Mouse CXCR2/IL‑8 RB PE‑conjugated Monoclonal Antibody (Catalog # FAB2164P) or (B) Rat IgG1 Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins
Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB PE‑conjugated Monoclonal Antibody (Catalog # FAB2164P). Quadrant markers were set based on control antibody staining (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR2/IL-8 RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

  • Long Name:
    Interleukin 8 Receptor B
  • Entrez Gene IDs:
    3579 (Human); 12765 (Mouse); 29385 (Rat)
  • Alternate Names:
    beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; IL-8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Small molecule-based inhibition of MEK1/2 proteins dampens inflammatory responses to malaria, reduces parasite load, and mitigates pathogenic outcomes
    Authors: X Wu, KK Dayanand, RP Thylur, CC Norbury, DC Gowda
    J. Biol. Chem., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. IL33-mediated ILC2 activation and neutrophil IL5 production in the lung response after severe trauma: A reverse translation study from a human cohort to a mouse trauma model
    Authors: J Xu, J Guardado, R Hoffman, H Xu, R Namas, Y Vodovotz, L Xu, M Ramadan, J Brown, HR Turnquist, TR Billiar
    PLoS Med., 2017;14(7):e1002365.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. CXCL1 is critical for pre-metastatic niche formation and metastasis in colorectal cancer
    Authors: D Wang, H Sun, J Wei, B Cen, RN DuBois
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  4. Mincle activation enhances neutrophil migration and resistance to polymicrobial septic peritonitis
    Authors: WB Lee, JJ Yan, JS Kang, Q Zhang, WY Choi, LK Kim, YJ Kim
    Sci Rep, 2017;7(0):41106.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  5. A Broad-Spectrum Chemokine-Binding Protein of Bovine Papular Stomatitis Virus Inhibits Neutrophil and Monocyte Infiltration in Inflammatory and Wound Models of Mouse Skin
    PLoS ONE, 2016;11(12):e0168007.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  6. B7H3 ameliorates LPS-induced acute lung injury via attenuation of neutrophil migration and infiltration
    Sci Rep, 2016;6(0):31284.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. CXCR1/2 inhibition blocks and reverses type 1 diabetes in mice.
    Authors: Citro A, Valle A, Cantarelli E, Mercalli A, Pellegrini S, Liberati D, Daffonchio L, Kastsiuchenka O, Ruffini P, Battaglia M, Allegretti M, Piemonti L
    Diabetes, 2015;64(4):1329-40.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice.
    Authors: Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F
    Mucosal Immunol, 2014;7(5):1079-93.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. alpha-Acid glycoprotein decreases neutrophil migration and increases susceptibility to sepsis in diabetic mice.
    Authors: Spiller F, Carlos D, Souto FO, de Freitas A, Soares FS, Vieira SM, Paula FJ, Alves-Filho JC, Cunha FQ
    Diabetes, 2012;61(6):1584-91.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. IL-22 signaling contributes to West Nile encephalitis pathogenesis.
    Authors: Wang, Penghua, Bai, Fengwei, Zenewicz, Lauren A, Dai, Jianfeng, Gate, David, Cheng, Gong, Yang, Long, Qian, Feng, Yuan, Xiaoling, Montgomery, Ruth R, Flavell, Richard, Town, Terrence, Fikrig, Erol
    PLoS ONE, 2012;7(8):e44153.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012;97(6):818-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. CRTH2 Is A Critical Regulator of Neutrophil Migration and Resistance to Polymicrobial Sepsis.
    Authors: Ishii M, Asano K, Namkoong H, Tasaka S, Mizoguchi K, Asami T, Kamata H, Kimizuka Y, Fujiwara H, Funatsu Y, Kagawa S, Miyata J, Ishii K, Nakamura M, Hirai H, Nagata K, Kunkel SL, Hasegawa N, Betsuyaku T
    J. Immunol., 2012;188(11):5655-64.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  13. Chemokine homeostasis vs. chemokine presentation during severe acute lung injury: the other side of the Duffy antigen receptor for chemokines.
    Authors: Zarbock A, Bishop J, Muller H, Schmolke M, Buschmann K, Van Aken H, Singbartl K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(3):L462-71.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. Lipid-cytokine-chemokine cascade drives neutrophil recruitment in a murine model of inflammatory arthritis.
    Authors: Chou RC, Kim ND, Sadik CD
    Immunity, 2010;33(2):266-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. Stimulation of the molecule 4-1BB enhances host defense against Listeria monocytogenes infection in mice by inducing rapid infiltration and activation of neutrophils and monocytes.
    Authors: Lee SC, Ju SA, Sung BH, Heo SK, Cho HR, Lee EA, Kim JD, Lee IH, Park SM, Nguyen QT, Suh JH, Kim BS
    Infect. Immun., 2009;77(5):2168-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Duffy antigen modifies the chemokine response in human endotoxemia.
    Authors: Mayr FB, Spiel AO, Leitner JM, Firbas C, Kliegel T, Jilma-Stohlawetz P, Derendorf H, Jilma B
    Crit. Care Med., 2008;36(1):159-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Dendritic cells in islets of Langerhans constitutively present beta cell-derived peptides bound to their class II MHC molecules.
    Authors: Calderon B, Suri A, Miller MJ
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(16):6121-6.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  18. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
    Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
    Eur. J. Immunol., 2007;37(10):2753-63.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
    Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
    Blood, 2007;109(7):2936-43.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  20. STAT3 governs distinct pathways in emergency granulopoiesis and mature neutrophils.
    Authors: Panopoulos AD, Zhang L, Snow JW, Jones DM, Smith AM, El Kasmi KC, Liu F, Goldsmith MA, Link DC, Murray PJ, Watowich SS
    Blood, 2006;108(12):3682-90.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 20 Citations
Isotype Controls
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Rat IgG2A PE-conjugated Antibody

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Flow Cytometry Staining Buffer (1X)

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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

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Average Rating: 4.5 (Based on 2 reviews)

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Flow Cytometry Mouse CXCR2/IL-8 RB PE-conjugated Antibody FAB2164P
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 Flow Mouse Anonymous 08/18/2017
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Flow Cytometry Mouse CXCR2/IL-8 RB PE-conjugated Antibody FAB2164P
Flow Cytometry: Mouse CXCR2/IL-8 RB PE-conjugated Antibody [FAB2164P]

Summary

ApplicationFlow Cytometry
Sample TestedMouse splenocytes
SpeciesMouse
  Very Good
  Anonymous 04/25/2017
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