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Mouse CXCR4 APC-conjugated Antibody

  (2 citations)     
Datasheet / CoA / SDS
Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry.

Flow Cytometry

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Product Details
Citations (2)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 247506
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with mouse CXCR4
    Met1-Ser359
    Accession # P70658
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Product Datasheets
Product Datasheet

COA

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SDS
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Flow Cytometry
Flow Cytometry CXCR4 Antibody (247506) [Allophycocyanin]
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Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB21651A) or (B) Rat IgG2B isotype control antibody (Catalog # IC013A) and Rat anti-Mouse CD8 alpha PE-conjugated Monoclonal Antibody (Catalog # FAB116P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CXCR4

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.

  • Entrez Gene IDs:
    7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
  • Alternate Names:
    CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
 Applications
 Sample Type
  1. Osteoblasts protect AML cells from SDF-1-induced apoptosis.
    Authors: Kremer K, Dudakovic A, McGee-Lawrence M, Philips R, Hess A, Smith B, van Wijnen A, Karp J, Kaufmann S, Westendorf J, Hedin K
    J Cell Biochem, 2014;115(6):1128-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Application of the chemokine CXCL12 expression plasmid restores wound healing to near normal in a diabetic mouse model.
    Authors: Restivo TE, Mace KA, Harken AH, Young DM
    J Trauma, 2010;69(2):392-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B APC‑conjugated Isotype Control

 Ctrl IC013A 4  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

 Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

 Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

 Flow FC002 1
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