Mouse CXCR4 APC-conjugated Antibody

FAB21651A 2 Citations Datasheet / COA / SDS

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FAB21651A-100UG
FAB21651A-025UG

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Mouse CXCR4 APC-conjugated Antibody in Flow Cytometry

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Citations (2)

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Mouse CXCR4 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.

Source

Monoclonal Rat IgG_2B Clone # 247506

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with mouse CXCR4
Met1-Ser359
Accession # P70658

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CXCR4 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with (A) Rat Anti-Mouse CXCR4 APC-conjugated Monoclonal Antibody (Catalog # FAB21651A) or (B) Rat IgG_2Bisotype control antibody (Catalog # IC013A) and Rat anti-Mouse CD8 alpha PE-conjugated Monoclonal Antibody (Catalog # FAB116P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCR4

CXCR4, also known as CD184, is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and -2 viral entry into cells. While primarily a membrane protein, CXCR4 undergoes trafficking and internalization in response to stimulation with phorbol esters and ligand (1). Cytoplasmic and nuclear localization of CXCR4 has been observed in colorectal and renal carcinomas (2,3) and it has been used as the basis of prognosis and metastatic state (3,4,5).

References

Orsini, M.J. et al. (1999) J. Biol. Chem. 274:31076.
Zagzag, D. et al. (2005) Cancer Res. 65:6178.
Speetjens, F.M. et al. (2009) Cancer Microenvironment 2:1.
Wang, L. et al. (2009) Oncology Reports 22:1333.
Amara, S. et al. (2015) Cancer Biomark. 15:869.

Entrez Gene IDs

7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)

Alternate Names

CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201E; D2S201ESDF-1 receptor; FB22; Fusin; HM89; HM89NPYRL; HSY3RR; LAP3; LAP-3; LCR1; LESTR; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3R; NPY3RWHIM; NPYR; NPYRL; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; WHIMS

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Citations for Mouse CXCR4 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Osteoblasts protect AML cells from SDF-1-induced apoptosis.
Authors: Kremer K, Dudakovic A, McGee-Lawrence M, Philips R, Hess A, Smith B, van Wijnen A, Karp J, Kaufmann S, Westendorf J, Hedin K
J Cell Biochem, 2014;115(6):1128-37.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Application of the chemokine CXCL12 expression plasmid restores wound healing to near normal in a diabetic mouse model.
Authors: Restivo TE, Mace KA, Harken AH, Young DM
J Trauma, 2010;69(2):392-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry