hh Mouse CXCR4 Antibody MAB21651-100: R&D Systems

Mouse CXCR4 Antibody

(24 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCR4 transfectants. Does not stain irrelevant transfectants.
  • Source
    Monoclonal Rat IgG2B Clone # 247506
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Y3 rat myeloid cell line transfected with mouse CXCR4
    Met1-Ser359
    Accession # P70658
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse thymocytes
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CXCL12/SDF‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR4. The Neutralization Dose (ND50) is typically 8‑40 µg/mL in the presence of 10 ng/mL Recombinant Mouse CXCL12/SDF‑1 alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL12/SDF‑1 alpha  and Neutralization by Mouse CXCR4 Antibody. Recombinant Mouse CXCL12/SDF‑1 alpha (Catalog # 460-SD) chemo­attracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL12/ SDF‑1 alpha (10 ng/mL) is neutral­ized (green line) by increasing concentrations of Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651). The ND50 is typically 8-40 µg/mL.
Immunocytochemistry
CXCR4 in Mouse Splenocytes. CXCR4 was detected in immersion fixed mouse splenocytes using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature.  Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunocytochemistry
CXCR4 in Mouse Neural Progenitor Cells. CXCR4 was detected in immersion fixed mouse neural progenitor cells using 10 µg/mL Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Immunohistochemistry
CXCR4 in Mouse Spleen. CXCR4 was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR4 Monoclonal Antibody (Catalog # MAB21651) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR4

CXCR4 is a G-protein-linked seven transmembrane spanning receptor that binds stromal cell-derived factor-1 (SDF-1). CXCR4 acts as a co-factor for T-cell tropic HIV-1 and-2 viral entry into cells.

  • Entrez Gene IDs:
    7852 (Human); 12767 (Mouse); 60628 (Rat); 483900 (Canine); 493676 (Feline)
  • Alternate Names:
    CD184; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C motif), receptor 4 (fusin); C-X-C chemokine receptor type 4; CXCR4; CXC-R4; CXCR-4; D2S201ESDF-1 receptor; FB22; Fusin; HM89NPYRL; HSY3RR; LAP3; LCR1; LESTRCD184 antigen; Leukocyte-derived seven transmembrane domain receptor; leukocyte-derived seven-transmembrane-domain receptor; lipopolysaccharide-associated protein 3; neuropeptide Y receptor Y3; NPY3RWHIM; NPYR; NPYY3R; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

24 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Proinflammatory CXCL12-CXCR4/CXCR7 signaling axis drives Myc-induced prostate cancer in obese mice
    Authors: A Saha, S Ahn, J Blando, F Su, MG Kolonin, J DiGiovanni
    Cancer Res., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. CXCL12 enhances angiogenesis through CXCR7 activation in human umbilical vein endothelial cells
    Authors: M Zhang, L Qiu, Y Zhang, D Xu, JC Zheng, L Jiang
    Sci Rep, 2017;7(1):8289.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  3. CXCR4 signaling in macrophages contributes to periodontal mechanical hypersensitivity in Porphyromonas gingivalis-induced periodontitis in mice
    Authors: H Nagashima, M Shinoda, K Honda, N Kamio, M Watanabe, T Suzuki, N Sugano, S Sato, K Iwata
    Mol Pain, 2017;13(0):1744806916689.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  4. Natural amines inhibit activation of human plasmacytoid dendritic cells through CXCR4 engagement
    Authors: N Smith, N Pietrancos, S Davidson, J Dutrieux, L Chauveau, P Cutolo, M Dy, D Scott-Alga, B Manoury, O Zirafi, I McCort-Tra, T Durroux, F Bachelerie, O Schwartz, J Münch, A Wack, S Nisole, JP Herbeuval
    Nat Commun, 2017;8(0):14253.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  5. Loss of Ranbp2 in motor neurons causes the disruption of nucleocytoplasmic and chemokine signaling and proteostasis of hnRNPH3 and Mmp28, and the development of amyotrophic lateral sclerosis (ALS)-like syndromes
    Authors: KI Cho, D Yoon, S Qiu, Z Danziger, WM Grill, WC Wetsel, PA Ferreira
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  6. A gonogenic stimulated transition of mouse embryonic stem cells with enhanced control of diverse differentiation pathways
    Sci Rep, 2016;6(0):25104.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  7. NKG2D ligand overexpression in lupus nephritis correlates with increased NK cell activity and differentiation in kidneys but not in the periphery.
    Authors: Spada R, Rojas J, Perez-Yague S, Mulens V, Cannata-Ortiz P, Bragado R, Barber D
    J Leukoc Biol, 2015;97(3):583-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  8. CXCR7 Mediates Neural Progenitor Cells Migration to CXCL12 Independent of CXCR4.
    Authors: Chen Q, Zhang M, Li Y, Xu D, Wang Y, Song A, Zhu B, Huang Y, Zheng J
    Stem Cells, 2015;33(8):2574-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  9. An important role of the SDF-1/CXCR4 axis in chronic skin inflammation.
    Authors: Zgraggen, Silvana, Huggenberger, Reto, Kerl, Katrin, Detmar, Michael
    PLoS ONE, 2014;9(4):e93665.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  10. Systemic neutralization of IL-17A significantly reduces breast cancer associated metastasis in arthritic mice by reducing CXCL12/SDF-1 expression in the metastatic niches.
    Authors: Roy, Lopamudr, Sahraei, Mahnaz, Schettini, Jorge L, Gruber, Helen E, Besmer, Dahlia M, Mukherjee, Pinku
    BMC Cancer, 2014;14(0):225.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  11. Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment.
    Authors: DeNiro M, Al-Mohanna F
    PLoS ONE, 2014;9(7):e101602.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  12. Protein z exerts pro-angiogenic effects and upregulates CXCR4.
    Authors: Butschkau, Antje, Wagner, Nana-Mar, Genz, Berit, Vollmar, Brigitte
    PLoS ONE, 2014;9(12):e113554.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  13. Mechanisms of adhesion and subsequent actions of a haematopoietic stem cell line, HPC-7, in the injured murine intestinal microcirculation in vivo.
    Authors: Kavanagh D, Yemm A, Zhao Y, Frampton J, Kalia N
    PLoS ONE, 2013;8(3):e59150.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. CXCL12-CXCR4 signaling is required for the maintenance of mouse spermatogonial stem cells.
    Authors: Yang, Qi-En, Kim, Dongwon, Kaucher, Amy, Oatley, Melissa, Oatley, Jon M
    J Cell Sci, 2013;126(0):1009-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  15. The role of SDF-1-CXCR4/CXCR7 axis in the therapeutic effects of hypoxia-preconditioned mesenchymal stem cells for renal ischemia/reperfusion injury.
    Authors: Liu H, Liu S, Li Y, Wang X, Xue W, Ge G, Luo X
    PLoS ONE, 2012;7(4):e34608.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  16. Influence of hypoxia-inducible factor 1-alpha on dendritic cell differentiation and migration.
    Authors: Kohler T, Reizis B, Johnson RS, Weighardt H, Forster I
    Eur. J. Immunol., 2012;42(5):1226-36.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  17. MicroRNA126 contributes to granulocyte colony-stimulating factor-induced hematopoietic progenitor cell mobilization by reducing the expression of vascular cell adhesion molecule 1.
    Authors: Salvucci O, Jiang K, Gasperini P
    Haematologica, 2012;97(6):818-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  18. IL-1alpha and IL-1beta recruit different myeloid cells and promote different stages of sterile inflammation.
    Authors: Rider P, Carmi Y, Guttman O, Braiman A, Cohen I, Voronov E, White MR, Dinarello CA, Apte RN
    J. Immunol., 2011;187(9):4835-43.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  19. Hypoxic preconditioning advances CXCR4 and CXCR7 expression by activating HIF-1alpha in MSCs.
    Authors: Liu H, Xue W, Ge G, Luo X, Li Y, Xiang H, Ding X, Tian P, Tian X
    Biochem. Biophys. Res. Commun., 2010;401(4):509-15.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  20. Microarray analysis of retinal endothelial tip cells identifies CXCR4 as a mediator of tip cell morphology and branching.
    Authors: Strasser GA, Kaminker JS, Tessier-Lavigne M
    Blood, 2010;115(24):5102-10.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  21. Paracrine modulation of CXCR4 by IGF-1 and VEGF: implications for choroidal neovascularization.
    Authors: Sengupta N, Afzal A, Caballero S, Chang KH, Shaw LC, Pang JJ, Bond VC, Bhutto I, Baba T, Lutty GA, Grant MB
    Invest. Ophthalmol. Vis. Sci., 2010;51(5):2697-704.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. Rosuvastatin reduces atherosclerotic lesions and promotes progenitor cell mobilisation and recruitment in apolipoprotein E knockout mice.
    Authors: Schroeter MR, Humboldt T, Schafer K, Konstantinides S
    Atherosclerosis, 2009;205(1):63-73.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  23. The angiogenic response is dictated by beta3 integrin on bone marrow-derived cells.
    Authors: Feng W, McCabe NP, Mahabeleshwar GH, Somanath PR, Phillips DR, Byzova TV
    J. Cell Biol., 2008;183(6):1145-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  24. Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells.
    Authors: Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL
    Cancer Res., 2008;68(6):1889-95.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Expand to show all 24 Citations
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