|Detection of CXCR5 in Mouse splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1217F) and either (A) Rat Anti-Mouse CXCR5 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB6198C) or (B) Rat IgG2A PerCP Isotype Control (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.|
CXCR5 (CXC chemokine receptor 5; also known as BLR-1, NLR and CD185) is a 55-60 kDa member of the G-protein coupled receptor 1 family. It is expressed on select cell types, including granule and Purkinje cell neurons, embryonic CD4+ CD3- IL-7R alpha + precursor cells, B cells and follicular T helper cells. CXCR5 selectively binds BLC/CXCL13. This appears to both promote embryonic lymph node development and, in the adult, direct expressing cells to positions that optimize antigen presentation and antibody production. Mouse CXCR5 is a 7-transmembrane glycoprotein that is 374 amino acids (aa) in length. It contains a 57 aa N-terminal extracellular region plus a 47 aa C-terminal cytoplasmic domain. Over aa 1-57, mouse CXCR5 shares 84% and 47% aa identity with rat and human CXCR5, respectively.
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