Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # RA3-6B2
View all B220/CD45R Primary Antibodies »

Product Specifications

Immunogen

Abelson mouse leukemia virus-induced pre-B tumor cells

Specificity

Detects mouse B220/CD45R.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse B220/CD45R Fluorescein‑conjugated Antibody

Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse B220/CD45R Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample:   Mouse splenocytes

Reviewed Applications

Read 2 reviews rated 5 using FAB1217F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References

  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.

Long Name

Cluster of Differentiation 45

Alternate Names

CD45R

Entrez Gene IDs

5788 (Human); 19264 (Mouse); 24699 (Rat)

Gene Symbol

PTPRC

Additional B220/CD45R Products

Product Documents for Mouse B220/CD45R Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse B220/CD45R Fluorescein‑conjugated Antibody

For research use only

Citations for Mouse B220/CD45R Fluorescein‑conjugated Antibody

Customer Reviews for Mouse B220/CD45R Fluorescein‑conjugated Antibody (2)

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  • Mouse B220/CD45R Fluorescein-conjugated Antibody
    Name: Jim Hsiao
    Application: Flow Cytometry
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 03/20/2018
    The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.
    Mouse B220/CD45R Fluorescein‑conjugated Antibody FAB1217F
  • Mouse B220/CD45R Fluorescein-conjugated Antibody
    Name: James Xiao
    Application: Flow Cytometry
    Sample Tested: Mouse spleen cells
    Species: IL1-R KO Mouse
    Verified Customer | Posted 01/03/2018
    Cells were pre-gated with live/dead and FSC-A/W to exclude dead cells and cell doublets.
    The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.
    Mouse B220/CD45R Fluorescein‑conjugated Antibody FAB1217F

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