Mouse B220/CD45R Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1217F

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Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Mouse B220/CD45R Fluorescein-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R.
Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F) or (B) Rat IgG2AFluorescein Isotype Control (Catalog # IC006F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B220/CD45R

B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References
  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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Citations for Mouse B220/CD45R Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Infection of Female BWF1 Lupus Mice with Malaria Parasite Attenuates B Cell Autoreactivity by Modulating the CXCL12/CXCR4 Axis and Its Downstream Signals PI3K/AKT, NFkappaB and ERK.
    Authors: Badr G, Sayed A, Abdel-Maksoud M, Mohamed A, El-Amir A, Abdel-Ghaffar F, Al-Quraishy S, Mahmoud M
    PLoS ONE, 2015-04-24;10(4):e0125340.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Nematode Asparaginyl-tRNA Synthetase Resolves Intestinal Inflammation in Mice with T-Cell Transfer Colitis
    Authors: Michael A. Kron, Ahmed Metwali, Sanja Vodanovic-Jankovic, David Elliott
    Clinical and Vaccine Immunology
  3. Infection with Anaplasma phagocytophilum induces multilineage alterations in hematopoietic progenitor cells and peripheral blood cells.
    Authors: Johns JL, Macnamara KC, Walker NJ, Winslow GM, Borjesson DL
    Infect. Immun., 2009-06-29;77(9):4070-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse B220/CD45R Fluorescein-conjugated Antibody

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Mouse B220/CD45R Fluorescein-conjugated Antibody
By Jim Hsiao on 03/20/2018
Application: Flow Sample Tested: Splenocytes Species: Mouse

The single cell suspension was isolated from a spleen of an IL1-R KO (knockout) mouse and stained with the FITC-Conjugated CD45R antibody (1:100) for 30 minutes. Cells were analyzed by flow cytometer.