Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry, Western Blot, Neutralization, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 466305
View all Cyr61/CCN1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Cyr61/CCN1
Asp176-Gly281
Accession # P18406

Specificity

Detects mouse Cyr61/CCN1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Cyr61 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Cyr61/CCN1 Antibody

Cyr61/CCN1 antibody in Mouse Trigeminal Ganglia by Immunohistochemistry (IHC-Fr).

Cyr61/CCN1 in Mouse Trigeminal Ganglia.

Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 µg/mL Rat Anti-Mouse Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB4864) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Mouse Cyr61/CCN1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse trigeminal ganglia

Reviewed Applications

Read 2 reviews rated 4.5 using MAB4864 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyr61/CCN1

Cyr61, also known as IGFBP-10 and CCN1, is a 50 kDa secreted matrix- and cell-associated glycoprotein that regulates the growth and adhesion of vascular endothelial cells, fibroblasts, and monocytes. Cyr61 interacts with cells that express integrins  alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1. Cyr61 is cleaved by plasmin within its VWF domain which generates an N-terminal fragment that is not associated with the matrix but retains the ability to induce endothelial cell migration. Cyr61 induces VEGF upregulation, angiogenesis, and tumorigenesis. Between amino acids 176-281, mouse Cyr61 shares 87% and 97% amino acid sequence identity with human and rat Cyr61, respectively.

Long Name

Cysteine-Rich, Angiogenic Inducer 61

Alternate Names

CCN1, GIG1, IGFBP-10

Entrez Gene IDs

3491 (Human); 16007 (Mouse); 83476 (Rat)

Gene Symbol

CCN1

UniProt

P18406

Additional Cyr61/CCN1 Products

Product Documents for Mouse Cyr61/CCN1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cyr61/CCN1 Antibody

For research use only

Citations for Mouse Cyr61/CCN1 Antibody

Customer Reviews for Mouse Cyr61/CCN1 Antibody (2)

4.5 out of 5
2 Customer Ratings
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Customer Images

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  • Mouse Cyr61/CCN1 Antibody
    Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: C3H10T1/2 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 05/20/2019
  • Mouse Cyr61/CCN1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 07/15/2018
    Mouse Cyr61/CCN1 Antibody MAB4864

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Protocols

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