Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 227807
View all Cystatin B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Cystatin B
Met2-Phe98
Accession # Q62426

Specificity

Detects mouse Cystatin B in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) Cystatin B is observed and no cross-reactivity with rhCystatin A, C, D, E/M, F, S, SA, rhFetuin A, rhFetuin B, rhHPRG, or rhKininogen is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Cystatin B Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spinal cord

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cystatin B

Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Cystatin B-deficient mice have increased expression of proteolysis, apoptosis and glial activation genes, which is consistent with the pathology found in the mouse model of human progressive myoclonus epilepsy (EPM1) (3). The mouse Cystatin B consists of 98 amino acid residues (4).

References

  1. Abrahamson, M. (1994) Methods Enzymol. 244:685.
  2. Pol, E. and I. Bjork (1999) Biochemistry 38:10519.
  3. Lieuallen, K. et al. (2001) Hum. Mol. Genet. 10:1867.
  4. Pennacchio, L.A. and R.M. Myers (1996) Genome Res. 6:1103.

Alternate Names

CSTB, Stefin B

Entrez Gene IDs

1476 (Human); 13014 (Mouse)

Gene Symbol

CSTB

UniProt

Q62426

Additional Cystatin B Products

Product Documents for Mouse Cystatin B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Cystatin B Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

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Protocols

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