Detection of Mouse Cystatin D/Cst10 by Western Blot.
Western blot shows lysates of mouse salivary gland tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Cystatin D/Cst10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6255) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cystatin D/Cst10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin D/Cst10
Cystatin D (Cystatin 10; also Carminerin) is a 17 kDa member of the type 2 cystatin family of proteins. It is expressed in salivary gland, skin, prehypertrophic and hypertrophic chondrocytes. Within the context of endochondral ossification, it may both induce chondrocyte apoptosis and initiate the process of cartilage calcification. Although Cystatin D contains a key cystatin motif (Gln-x-Val-x-Gly), Cystatin D does not appear to inhibit cysteine proteases. Mouse Cystatin D is 148 amino acids (aa) in length. It contains one cystatin-like domain (aa 39-145). There are two potential splice variants. One shows a deletion of aa 84-121, while a second shows a Val substitution for aa 122-148. Full-length mouse Cystatin D shares 78% aa identity with rat Cystatin D. There appears to be no direct human counterpart to mouse Cystatin D, as both human cystatin-C and -D share less that 40% aa identity with mouse Cystatin D.
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