Mouse DC‑SIGN/CD209 PE‑conjugated Antibody

Name: Mouse DC‑SIGN/CD209 PE‑conjugated Antibody
Brand: R&D Systems
SKU: FAB8345P
Price: 189 USD
Availability: InStock

R&D Systems | Catalog # FAB8345P

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Product Documents

Recombinant Monoclonal Antibody.

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Product Specifications
Scientific Data
Applications
Spectra Viewer
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Recombinant Monoclonal Rat IgG_2B Clone # 902404

View all DC-SIGN/CD209 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with mouse DC‑SIGN/CD209
Met1-Lys238
Accession # Q91ZX1

Specificity

Detects mouse DC‑SIGN/CD209 in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Mouse DC‑SIGN/CD209 PE‑conjugated Antibody

Detection of DC‑SIGN/CD209 in Mouse Bone Marrow-derived Dendritic Cells by Flow Cytometry.

Mouse bone marrow-derived dendritic cells were stained with Rat Anti-Mouse DC-SIGN/CD209 PE-conjugated Monoclonal Antibody (Catalog # FAB8345P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of DC‑SIGN/CD209 in CHO Chinese Hamster Cell Line Transfected with Mouse DC-SIGN/CD209 by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Rat Anti-Mouse DC-SIGN/CD209 PE-conjugated Monoclonal Antibody (Catalog # FAB8345P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse DC‑SIGN/CD209 PE‑conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Mouse bone marrow-derived dendritic cells and CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca²⁺-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).