Mouse DC-SIGN/CD209 PE-conjugated Antibody

Recombinant Monoclonal Antibody.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse DC‑SIGN/CD209 in direct ELISAs.
  • Source
    Recombinant Monoclonal Rat IgG2B Clone # 902404
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse DC‑SIGN/CD209
    Met1-Lys238
    Accession # Q91ZX1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DC‑SIGN/CD209 in Mouse Bone Marrow-derived Dendritic Cells by Flow Cytometry. Mouse bone marrow-derived dendritic cells were stained with Rat Anti-Mouse DC‑SIGN/CD209 PE‑conjugated Monoclonal Antibody (Catalog # FAB8345P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of DC‑SIGN/CD209 in CHO Chinese Hamster Cell Line Transfected with Mouse DC-SIGN/CD209 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Rat Anti-Mouse DC‑SIGN/CD209 PE‑conjugated Monoclonal Antibody (Catalog # FAB8345P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DC-SIGN/CD209
Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).
  • References:
    1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
    2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.
    3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
    4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
    5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
    6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
  • Long Name:
    Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
  • Entrez Gene IDs:
    30835 (Human); 170786 (Mouse)
  • Alternate Names:
    CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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