Mouse DC-SIGN/CD209 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8345
MAB8345-SP
Mouse DC-SIGN/CD209 Antibody in Data
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Mouse DC-SIGN/CD209 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse DC‑SIGN/CD209 in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2B Clone # 902404
Purification
Protein A or G purified from cell culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with mouse DC‑SIGN/CD209
Met1-Lys238
Accession # Q91ZX1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of DC‑SIGN/CD209 in CHO Chinese Hamster Ovary Cell Line Transfected with Mouse DC-SIGN/CD209 by Flow Cytometry. View Larger

Detection of DC‑SIGN/CD209 in CHO Chinese Hamster Ovary Cell Line Transfected with Mouse DC-SIGN/CD209 by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse DC-SIGN/CD209 was stained with Rat Anti-Mouse DC-SIGN/CD209 Monoclonal Antibody (Catalog # MAB8345, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DC-SIGN/CD209

Mouse Dendritic Cell-specific ICAM-3 Grabbing Non-integrin (DC-SIGN)/CD209, also known as CD209 Antigen-like Protein A, is a member of the C-type lectin family (1). Mouse DC-SIGN/CD209 is a 33 kDa, 238 amino acid (aa) type II transmembrane protein (2). The extracellular region contains a Ca2+-dependent carbohydrate-binding lectin domain (2). In addition to the full-length mouse DC-SIGN/CD209, a second, truncated splice variant has been reported. DC-SIGN/CD209 is not well conserved between mouse and human with the extracellular domain sharing only 63% aa identity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2-4). DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation (2, 5, 6).

References
  1. Liu, W. et al. (2004) J. Biol. Chem. 279:18748.
  2. Caminschi, I. et al. (2001) Mol. Immunol. 38:365.
  3. Curtis, B.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:8356.
  4. Anthony, R.M. et al. (2008) Proc. Natl. Acad. Sci. USA 105:19571.
  5. Geijtenbeek, T.B. et al. (2000) Cell 100:575.
  6. Garcia-Vallejo, J.J. and Y. van Kooyk (2013) Trends Immunol. 34:482.
Long Name
Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1
Entrez Gene IDs
30835 (Human); 170786 (Mouse); 102121984 (Cynomolgus Monkey)
Alternate Names
CD209 antigendendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbingnon-integrin; CD209 molecule; CD209; CDSIGNHIV gpl20-binding protein; CLEC4L; CLEC4LC-type lectin domain family 4 member L; DCSIGN; DC-SIGN; DC-SIGN1; DC-SIGN1C-type lectin domain family 4, member L; DC-SIGNMGC129965; Dendritic cell-specific ICAM-3-grabbing non-integrin 1

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