Mouse Decorin Biotinylated Antibody Summary
Accession # P28654
Mouse Decorin Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Decorin in Mouse Dorsal Root Ganglia. Decorin was detected in perfusion fixed frozen sections of mouse dorsal root ganglia using 15 µg/mL Goat Anti-Mouse Dec-orin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1060) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).
- Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
- Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.
Citation for Mouse Decorin Biotinylated Antibody
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1 Citation: Showing 1 - 1
Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly
Authors: AC Daquinag, Z Gao, C Fussell, K Sun, MG Kolonin
Sample Types: Cell Lysates
Applications: Western Blot
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