Mouse Decorin Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1060
Decorin in Mouse Dorsal Root Ganglia.
1 Image
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Mouse Decorin Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Decorin in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross‑reactivity with recombinant human Decorin is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Decorin
Gly17-Lys354
Accession # P28654
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Decorin (Catalog # 1060-DE)
Immunohistochemistry
5-15 µg/mL
See below

Mouse Decorin Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Decorin Antibody (Catalog # AF1060)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Decorin antibody in Mouse Dorsal Root Ganglia by Immunohistochemistry (IHC-P) by Immunohistochemistry (IHC-Fr). View Larger

Decorin in Mouse Dorsal Root Ganglia. Decorin was detected in perfusion fixed frozen sections of mouse dorsal root ganglia using 15 µg/mL Goat Anti-Mouse Dec-orin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1060) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Decorin

Decorin is a small secreted chondroitin/dermatan sulfate proteoglycan belonging to the class I small leucine-rich proteoglycan family (SLRP). All SLRP family members are characterized by the N-terminal and C-terminal cysteine-rich regions, which flank the central region containing 10‑12 tandem leucine-rich repeats. In mouse Decorin, the glycosaminoglycan chain is O-linked to Ser34 in the N-terminal disulfide-bridged loop. Decorin binds to fibronectin, TGF-beta, type I and type II collagen. The binding of Decorin to these molecules is mediated via the core protein. Decorin plays a role in maintaining collagen fibrillogenesis. Depending on the cell context, Decorin can either block or augment the bioactivity of TGF-beta  Decorin induces growth suppression by activation of a signaling pathway that culminates in the blockade of the cell cycle machinery. Decorin can also induce fibroblast cytoskeletal and signalling changes that results in an increased cell migration (1, 2).

References
  1. Iozzo, R.V. (1998) Annu. Rev. Biochem. 67:609.
  2. Tufvesson, E. and G. Westergren-Thorsson (2003) J. Cell Science 116:4857.
Entrez Gene IDs
1634 (Human); 13179 (Mouse)
Alternate Names
Bone proteoglycan II; CSCD; DCN; decorin proteoglycan; Decorin; dermatan sulphate proteoglycans II; DSPG2; PG40; PGII; PG-II; PGS2; PG-S2; proteoglycan core protein; SLRR1B; SLRR1BPGS2; small leucine-rich protein 1B

Product Datasheets

You must select a language.

x

Citations for Mouse Decorin Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Absence of fibromodulin affects matrix composition, collagen deposition and cell turnover in healthy and fibrotic lung parenchyma.
    Authors: Rydell-Tormanen K, Andreasson K, Hesselstrand R, Westergren-Thorsson G
    Sci Rep, 2014-09-18;4(0):6383.
  2. 5‐HT 2B receptor antagonists attenuate myofibroblast differentiation and subsequent fibrotic responses in vitro and in vivo
    Authors: Anna Löfdahl, Kristina Rydell-Törmänen, Catharina Müller, C. Martina Holst, Lena Thiman, Gunilla Ekström et al.
    Physiological Reports
  3. Glycosaminoglycan Modification of Decorin Depends on MMP14 Activity and Regulates Collagen Assembly
    Authors: AC Daquinag, Z Gao, C Fussell, K Sun, MG Kolonin
    Cells, 2020-12-09;9(12):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse Decorin Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse Decorin Biotinylated Antibody and earn rewards!

Have you used Mouse Decorin Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review