Mouse Dectin-1/CLEC7A Antibody
Mouse Dectin-1/CLEC7A Antibody Summary
Accession # Q6QLQ4
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 ‑ 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 ‑ 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).
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Citation for Mouse Dectin-1/CLEC7A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Protein-tyrosine phosphatase Shp2 positively regulates macrophage oxidative burst.
Authors: Li X, Goodwin C, Nabinger S, Richine B, Yang Z, Hanenberg H, Ohnishi H, Matozaki T, Feng G, Chan R
J Biol Chem, 2015;290(7):3894-909.
Sample Types: Cell Lysates
Applications: Western Blot
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HEK cells were transfected with mouse Dectin1 isoform 2 and stained with the Rat anti-mouse Dectin1 clone 218838 at a concentration of 0.005 nM to 300 nM. The 218838 antibody was detected using a secondary DyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab')₂ fragment specific antibody. The binding affinity was calculated at EC50 = 64 nM. Binding was observed only in the higher concentrations of the antibody.
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Detection of stalkless mouse Dectin1 isoform B in transfected HEK cells by Flow Cytometry. HEK cells overexpressing the murine Dectin1B isoform (stalkless) were stained with the Rat anti-mouse
Dectin1 clone 218838 at 100 nM or the Rat IgG2B isotype control. Binding was detected with a secondary a-Rat IgG (H+L) Pacific blue antibody.