Mouse Dectin-1/CLEC7A Antibody

Catalog # Availability Size / Price Qty
MAB1756-SP
MAB1756-100
MAB1756-500
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (2)

Mouse Dectin-1/CLEC7A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Dectin-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Dectin‑1, rmDectin-2 alpha, or rhDLEC.
Source
Monoclonal Rat IgG2B Clone # 218838
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1
Phe69-Leu244
Accession # Q6QLQ4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Dectin‑1/CLEC7A (Catalog # 1756-DC)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 ‑ 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 ‑ 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References
  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
  4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
  5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
  6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs
64581 (Human); 56644 (Mouse)
Alternate Names
Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1

Product Datasheets

Citation for Mouse Dectin-1/CLEC7A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Protein-tyrosine phosphatase Shp2 positively regulates macrophage oxidative burst.
    Authors: Li X, Goodwin C, Nabinger S, Richine B, Yang Z, Hanenberg H, Ohnishi H, Matozaki T, Feng G, Chan R
    J Biol Chem, 2015;290(7):3894-909.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Mouse Dectin-1/CLEC7A Antibody

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Mouse Dectin-1/CLEC7A Antibody
By Anonymous on 11/28/2020
Application: Flow Sample Tested: 293T human embryonic kidney cell line Species: Mouse

HEK cells were transfected with mouse Dectin1 isoform 2 and stained with the Rat anti-mouse Dectin­1 clone 218838 at a concentration of 0.005 nM to 300 nM. The 218838 antibody was detected using a secondary DyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab')₂ fragment specific antibody. The binding affinity was calculated at EC50 = 64 nM. Binding was observed only in the higher concentrations of the antibody.
*RnD Systems Response: Thank you for reviewing our product. We are sorry to that that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.


Mouse Dectin-1/CLEC7A Antibody
By Anonymous on 10/13/2020
Application: Flow Sample Tested: Transfected HEK cells overexpressing mouse Dectin-1b Species: Mouse

Detection of stalkless mouse Dectin­1 isoform B in transfected HEK cells by Flow Cytometry. HEK cells overexpressing the murine Dectin1B isoform (stalkless) were stained with the Rat anti-mouse
Dectin­1 clone 218838 at 100 nM or the Rat IgG2B isotype control. Binding was detected with a secondary a-Rat IgG (H+L) Pacific blue antibody.