Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 218838
View all Dectin-1/CLEC7A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1
Phe69-Leu244
Accession # Q6QLQ4

Specificity

Detects mouse Dectin-1 in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with recombinant human (rh) Dectin‑1, rmDectin-2 alpha, or rhDLEC.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Dectin‑1/CLEC7A Antibody

Detection of Dectin-1/CLEC7A by Western Blot

Detection of Dectin-1/CLEC7A by Western Blot

beta -glucan expression and Dectin-1 signaling in A. fumigatus keratitis. Fungal beta -glucan expression and Dectin-1 protein activation in the cornea during Aspergillus infection were assessed by IHC and western blot analysis, respectively. A. 5µm central cornea sections at 6 and 24h post-infection were PASH stained to visualize swollen conidia (6h) and hyphae (24h), while beta -glucan expression was detected using a mouse anti-fungal beta -glucan antibody. B. Western blot analysis using anti-Dectin-1 antibody or C. anti-pSyk antibody was performed on cornea protein lysates from naïve, 10h PBS injected (trauma control), and 10hr A. fumigatus infected corneas. Data are representative of two independent experiments, with three corneas per time point. [Endo- endothelium Epi- epithelium, Neuts- casein-elicited peritoneal neutrophils. MACs- thioglycolate-elicited peritoneal, macrophages.] Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/20617171), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse Dectin‑1/CLEC7A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Dectin‑1/CLEC7A (Catalog # 1756-DC)

Reviewed Applications

Read 2 reviews rated 2.5 using MAB1756 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 ‑ 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 ‑ 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

References

  1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
  2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
  3. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157.
  4. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513.
  5. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. 
  6. Willment, J.A. et al. (2003) J. Immunol. 171:4569. 
  7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
  8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
  9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
  10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
  11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
  12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
  13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
  14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
  15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
  16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.

Alternate Names

CD369, CLEC7A, CLECSF12, Dectin1

Entrez Gene IDs

64581 (Human); 56644 (Mouse)

Gene Symbol

CLEC7A

UniProt

Q6QLQ4

Additional Dectin-1/CLEC7A Products

Product Documents for Mouse Dectin‑1/CLEC7A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Dectin‑1/CLEC7A Antibody

For research use only

Citations for Mouse Dectin‑1/CLEC7A Antibody

Customer Reviews for Mouse Dectin‑1/CLEC7A Antibody (2)

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2 Customer Ratings
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  • Mouse Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: 293T human embryonic kidney cell line
    Species: Mouse
    Verified Customer | Posted 11/28/2020
    HEK cells were transfected with mouse Dectin1 isoform 2 and stained with the Rat anti-mouse Dectin­1 clone 218838 at a concentration of 0.005 nM to 300 nM. The 218838 antibody was detected using a secondary DyLight 405 AffiniPure Mouse Anti-Rat IgG, F(ab')₂ fragment specific antibody. The binding affinity was calculated at EC50 = 64 nM. Binding was observed only in the higher concentrations of the antibody.
    Mouse Dectin‑1/CLEC7A Antibody MAB1756
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction. Thank you so much for taking the time to share your experience using RnD Systems Antibodies. We value your opinion and use the online reviews as testimonials from our customers, of the quality of our products. Our technical service representatives work hard to resolve any issues with our products when customers report they have not had success. The immunogen used for MAB1756 is Mouse myeloma cell line NS0-derived recombinant mouse Dectin-1 (Phe69-Leu244, Accession # Q6QLQ4), which is Dectin1 isoform 1. Dectin1 isoform 2 was transfected and used in customer’s assay, which misses aa69-aa113 compared to isoform 1. We have not done epitope mapping for MAB1756 and therefore we don’t know which part of Dectin1 isoform 1 this antibody binds. We have not tested whether MAB1756 is able to detect isoform 2. Tech Service tried to reach the customer and didn’t receive a response. We would appreciate if you would be willing to contact a technical service representative at techsupport@bio-techne.com and allow us to troubleshoot the results you observed with MAB1756, that you reported in your online review. We look forward to hearing from you.
  • Mouse Dectin-1/CLEC7A Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Transfected HEK cells overexpressing mouse Dectin-1b
    Species: Mouse
    Verified Customer | Posted 10/13/2020
    Detection of stalkless mouse Dectin­1 isoform B in transfected HEK cells by Flow Cytometry. HEK cells overexpressing the murine Dectin1B isoform (stalkless) were stained with the Rat anti-mouse Dectin­1 clone 218838 at 100 nM or the Rat IgG2B isotype control. Binding was detected with a secondary a-Rat IgG (H+L) Pacific blue antibody.
    Mouse Dectin‑1/CLEC7A Antibody MAB1756

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