Mouse Desert Hedgehog/Dhh N-Terminus Antibody

Catalog # Availability Size / Price Qty
MAB733
MAB733-SP
Product Details
Citations (1)
FAQs
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Mouse Desert Hedgehog/Dhh N-Terminus Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Desert Hedgehog/Dhh N-Terminus in direct ELISAs and Western blots. In Western blots, no cross‑reactivity with the N‑terminal peptides of recombinant human Shh or recombinant mouse (rm) Shh is observed.  Also, in direct ELISAs and Western blots, no cross‑reactivity with the C-terminal peptides of rmDhh, rmIhh, or rmShh is observed.
Source
Monoclonal Rat IgG2A Clone # 126925
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Desert Hedgehog/Dhh
Cys23-Gly396
Accession # Q61488
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Desert Hedgehog (C23II), N-Terminus (Catalog # 733-DH)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desert Hedgehog/Dhh

Desert Hedgehog (Dhh) belongs to the highly conserved Hedgehog family of proteins which are involved in multiple developmental processes. Hedgehogs are synthesized as 45 kDa precursors that are cleaved autocatalytically. The 19 kDa N‑terminal fragment remains membrane associated due to its cholesterol and palmitate modifications. Binding of this fragment to Patched receptors results in the loss of Patched repression of Smoothened signaling (1‑4). Dhh binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip) (5). Within the N-terminal peptide, mouse Dhh shares 97% and 100% amino acid (aa) sequence identity with human and rat Dhh, respectively. It shares 74% aa seqeuence identity with mouse Indian (Ihh) and Sonic hedgehog (Shh) (6, 7). Dhh is produced by Sertoli cells and is required for testis development and spermatogenesis (8, 9). It induces steroidogenic factor 1 which is instrumental in promoting Leydig cell differentiation (10, 11). It also promotes the deposition of basal lamina surrounding seminiferous tubules (8). In humans, mutations of Dhh are associated with pure gonadal dysgenesis (12). Dhh is expressed in the female by ovarian granulosa cells and the corpus luteum (13). Its up‑regulation in human ovarian cancer correlates positively with proliferative index and negatively with prognosis (14). Dhh is also expressed by Schwann cells and is up‑regulated following nerve injury (15, 16). It induces the expression of Patched and Hip in nerve fibroblasts and promotes the formation of the connective tissue sheath surrounding peripheral nerves (15).

References
  1. van den Brink, G.R. (2007) Physiol. Rev. 87:1343.
  2. Riobo, N.A. and D.R. Manning (2007) Biochem. J. 403:369.
  3. Porter, J.A. et al. (1995) Nature 374:363.
  4. Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. 95:13630.
  5. Pathi, S. et al. (2001) Mech. Dev. 106:107.
  6. Echelard, Y. et al. (1993) Cell 75:1417.
  7. Chang, D.T. et al. (1994) Development 120:3339.
  8. Pierucci-Alves, F. et al. (2001) Biol. Reprod. 65:1392.
  9. Bitgood, M.J. et al. (1996) Curr. Biol. 6:298.
  10. Yao, H.H.-C. et al. (2002) Genes Dev. 16:1433.
  11. Park, S.Y. et al. (2007) Endocrinology 148:3704.
  12. Canto, P. et al. (2004) J. Clin. Endocrinol. 89:4480.
  13. Russell, M.C. et al. (2007) Biol. Reprod. 77:226.
  14. Chen, X. et al. (2007) Cancer Sci. 98:68.
  15. Parmantier, E. et al. (1999) Neuron 23:713.
  16. Bajestan, S.N. et al. (2006) J. Neurobiol. 66:243.
Entrez Gene IDs
50846 (Human); 13363 (Mouse)
Alternate Names
desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; Desert Hedgehog; DHH; HHG-3MGC35145

Product Datasheets

Citation for Mouse Desert Hedgehog/Dhh N-Terminus Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Functionally Distinctive Ptch Receptors Establish Multimodal Hedgehog Signaling in the Tooth Epithelial Stem Cell Niche
    Authors: M Binder, P Chmielarz, PJ Mckinnon, LC Biggs, I Thesleff, A Balic
    Stem Cells, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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