Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DPPA4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse DPPA4
Asn125-Gly296
Accession # Q8CCG4

Specificity

Detects mouse DPPA4 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human DPPA4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse DPPA4 Antibody

DPPA4 antibody in D3 Mouse Cell Line by Immunocytochemistry (ICC).

DPPA4 in D3 Mouse Cell Line.

DPPA4 was detected in immersion fixed D3 mouse embryonic stem cell line using Goat Anti-Mouse DPPA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3730) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Goat IgG Secondary Antibody (green; Catalog # NL003) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse DPPA4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse DPPA4

Reviewed Applications

Read 1 review rated 5 using AF3730 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DPPA4

DPPA4 is a 33 kDa molecule that is expressed specifically in the early embryo, uncommitted cells of the germ line, and embryonic stem cells and is used as a marker of pluripotent cells. Mouse DPPA4 is 296 amino acids in length and contains an SAP (SAF-A/B, ACINUS, PIAS) putative DNA-binding domain. Two potential isoforms exist with deletions of amino acids 104‑120 and 69‑120, respectively. In the region used as an immunogen, mouse DPPA4 shows 57% amino acid sequence identity with human and 87% identity with rat DPPA4 proteins.

Long Name

Developmental Pluripotency Associated 4

Alternate Names

developmental pluripotency associated 4, developmental pluripotency-associated protein 4, FLJ10713,2410091M23Rik

Entrez Gene IDs

55211 (Human); 73693 (Mouse)

Gene Symbol

DPPA4

UniProt

Q8CCG4

Additional DPPA4 Products

Product Documents for Mouse DPPA4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse DPPA4 Antibody

For research use only

Citations for Mouse DPPA4 Antibody

Customer Reviews for Mouse DPPA4 Antibody (1)

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  • Mouse DPPA4 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Mouse iPS
    Species: Mouse
    Verified Customer | Posted 10/26/2015
    Specificity: Specific<br />Sensitivity: Sensitive<br />Buffer: PBS<br />Dilution: 1:250
    Mouse DPPA4 Antibody AF3730

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Protocols

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FAQs

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