Mouse EGF Antibody

(4 citations)
(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse EGF in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human EGF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse EGF
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse EGF (Catalog # 2028-EG)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 0.3 ng/mL Recombinant Mouse EGF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by EGF and Neutralization by Mouse EGF Antibody.

Recombinant Mouse EGF (Catalog # 2028-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse EGF (0.3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028). The ND50 is typically 0.02‑0.06 µg/mL.

Immunohistochemistry
EGF in Mouse Kidney. EGF was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse EGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2028) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: EGF
Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1217 amino acid (aa) mouse EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature mouse EGF shares 70% and 77% aa sequence identity with mature human and rat EGF. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.
  • References:
    1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
    2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
    3. Gray, A. et al. (1983) Nature 303:722.
    4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
    5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
    6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
    7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
    8. Qian, X. et al. (1999) J. Biol. Chem. 274:574.
  • Long Name:
    Epidermal Growth Factor
  • Entrez Gene IDs:
    1950 (Human); 13645 (Mouse); 25313 (Rat)
  • Alternate Names:
    beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. A critical role for NF2 and the Hippo pathway in branching morphogenesis
    Nat Commun, 2016;7(0):12309.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Neut
  2. VEGFR1-positive macrophages facilitate liver repair and sinusoidal reconstruction after hepatic ischemia/reperfusion injury.
    Authors: Ohkubo, Hirotoki, Ito, Yoshiya, Minamino, Tsutomu, Eshima, Koji, Kojo, Ken, Okizaki, Shin-ich, Hirata, Mitsuhir, Shibuya, Masabumi, Watanabe, Masahiko, Majima, Masataka
    PLoS ONE, 2014;9(8):e105533.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Leukotriene B4 type-1 receptor signaling promotes liver repair after hepatic ischemia/reperfusion injury through the enhancement of macrophage recruitment.
    Authors: Ohkubo, Hirotoki, Ito, Yoshiya, Minamino, Tsutomu, Mishima, Toshiaki, Hirata, Mitsuhir, Hosono, Kanako, Shibuya, Masabumi, Yokomizo, Takehiko, Shimizu, Takao, Watanabe, Masahiko, Majima, Masataka
    FASEB J, 2013;27(8):3132-43.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+CD11b(high)Gr1(low) macrophages.
    Authors: Schiechl G, Bauer B, Fuss I, Lang SA, Moser C, Ruemmele P, Rose-John S, Neurath MF, Geissler EK, Schlitt HJ, Strober W, Fichtner-Feigl S
    J. Clin. Invest., 2011;121(5):1692-708.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Mouse EGF Antibody AF2028
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Very Good
 WB Mouse Anonymous 05/08/2017
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Western Blot Mouse EGF Antibody AF2028
Western Blot: Mouse EGF Antibody [AF2028]

Summary

ApplicationWestern Blot
Sample TestedRecombinant protein
SpeciesMouse

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