Mouse Endocan/ESM-1 Antibody

Catalog # Availability Size / Price Qty
AF1999
AF1999-SP
Endocan/ESM‑1 in Mouse Embryo.
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Product Details
Citations (9)
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Mouse Endocan/ESM-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Endocan/ESM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Endocan is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM-1
Trp20-Arg184
Accession # Q9QYY7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Endocan/ESM‑1 (Catalog # 1999-EC)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND50) is typically 1-5 µg/mL in the presence of 25 µg/mL Recombinant Mouse Endocan/ESM‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Endocan/ESM-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Endocan/ESM‑1 in Mouse Embryo. Endocan/ESM-1 was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization Cell Adhesion Mediated by Endocan/ESM‑1 and Neutralization by Mouse Endocan/ESM‑1 Antibody. View Larger

Cell Adhesion Mediated by Endocan/ESM‑1 and Neutralization by Mouse Endocan/ESM‑1 Antibody. Recombinant Mouse Endocan/ESM-1 (Catalog # 1999-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM-1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999). The ND50 is typically 1-5 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endocan/ESM-1

Endocan (endothelial cell proteoglycan; also known as endothelial-cell specific molecule-1 [ESM-1]), is a 50 kDa, monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 ( alpha L beta 2) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).

References
  1. Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
  2. Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
  3. Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
  4. Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
  5. Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
  6. Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
  7. Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.
Entrez Gene IDs
11082 (Human); 71690 (Mouse)
Alternate Names
Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6

Product Datasheets

Citations for Mouse Endocan/ESM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Limited hyperoxia-induced proliferative retinopathy: A model of persistent retinal vascular dysfunction, preretinal fibrosis and hyaloidal vascular reprogramming for retinal rescue
    Authors: T Tedeschi, K Lee, W Zhu, AA Fawzi
    PLoS ONE, 2022;17(4):e0267576.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis
    Authors: TS Malinova, A Angulo-Ura, J Nüchel, M Tauber, MM van der St, V Janssen, A de Haan, AG Groenen, M Tebbens, M Graupera, M Plomann, S Huveneers
    Nature Communications, 2021;12(1):2610.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Lack of WWC2 Protein Leads to Aberrant Angiogenesis in Postnatal Mice
    Authors: VC Brücher, C Egbring, T Plagemann, PI Nedvetsky, V Höffken, H Pavenstädt, N Eter, J Kremerskot, P Heiduschka
    International Journal of Molecular Sciences, 2021;22(10):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs
    Authors: R Ichijo, M Kabata, H Kidoya, F Muramatsu, R Ishibashi, K Abe, K Tsutsui, H Kubo, Y Iizuka, S Kitano, H Miyachi, Y Kubota, H Fujiwara, A Sada, T Yamamoto, F Toyoshima
    Science Advances, 2021;7(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Blocking endothelial apoptosis revascularises the retina in a model of ischemic retinopathy
    Authors: ZL Grant, L Whitehead, VHY Wong, Z He, RY Yan, AR Miles, AV Benest, DO Bates, C Prahst, K Bentley, BV Bui, RC Symons, L Coultas
    J. Clin. Invest., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Decreased expression of endothelial cell specific molecule-1 in lung tissue in emphysematous mice and stable COPD patients
    Authors: Y Zhang, P Chen, S Cai, J Li, Y Chen
    Iranian journal of basic medical sciences, 2020;23(12):1610-1617.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Endocan Blockade Suppresses Experimental Ocular Neovascularization in Mice
    Authors: T Su, Y Zhong, AM Demetriade, J Shen, A Sui, Y Yao, Y Gao, Y Zhu, X Shen, B Xie
    Invest. Ophthalmol. Vis. Sci., 2018;59(2):930-939.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Integrin beta1 controls VE-cadherin localization and blood vessel stability.
    Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
    Nat Commun, 2015;6(0):6429.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis.
    Authors: Shin JW, Huggenberger R, Detmar M
    Blood, 2008;112(6):2318-26.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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