Mouse Endocan/ESM-1 Antibody

Catalog #: AF1999
10 Citations Datasheet / COA / SDS

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AF1999
AF1999-SP

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Citations (10)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Endocan/ESM-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Endocan/ESM-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant human Endocan is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Endocan/ESM-1
Trp20-Arg184
Accession # Q9QYY7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Endocan/ESM‑1 (Catalog # 1999-EC)

Immunohistochemistry

5-15 µg/mL

See below

Neutralization

Measured by its ability to neutralize Endocan/ESM‑1-mediated adhesion of the Jurkat human acute T cell leukemia cell line. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 25 µg/mL Recombinant Mouse Endocan/ESM‑1.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Endocan/ESM‑1 in Mouse Embryo. Endocan/ESM-1 was detected in perfusion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the retina. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization

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Cell Adhesion Mediated by Endocan/ESM‑1 and Neutralization by Mouse Endocan/ESM‑1 Antibody. Recombinant Mouse Endocan/ESM-1 (Catalog # 1999-EC), immobilized onto a microplate, supports the adhesion of the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Mouse Endocan/ESM-1 (25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Endocan/ESM-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1999). The ND₅₀ is typically 1-5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endocan/ESM-1

Endocan (endothelial cell proteoglycan; also known as endothelial-cell specific molecule-1 [ESM-1]), is a 50 kDa, monomeric, secreted, cysteine-rich proteoglycan identified initially in endothelial cells of the kidney and lung (1). Mouse Endocan is synthesized as a 184 amino acid (aa) precursor that contains a 21 aa signal sequence and a 20 kDa, 163 aa mature region (2). The N-terminal 2/3 of the molecule contains 18 cysteine residues and there are no potential N-linked glycosylation sites. Based on human Endocan, there are at least two potential O-linked glycosylation sites, one of which will likely be utilized on Ser at position # 136 of the mature molecule (3). The posttranslational modification is approximately 30 kDa in size. It consists of a single dermatan sulfate chain that contains 4-O sulfated N-acetyl galactosamine with alpha -iduronate. This chain is suggested to bind HGF and contribute to HGF mitogenic activity (4). Mature mouse Endocan is 96% and 74% aa identical to rat and human Endocan, respectively. In human, there is a potential for an alternate splice variant. It shows a deletion of aa’s 82 through 131, a range which would not remove the dermatan sulfate attachment site (4). It is not known if such a splice form exists in mouse. Endocan is expressed by endothelial cells, adipocytes, bronchial epithelium and distal renal tubular epithelium (1, 5, 6). It is upregulated by TNF-alpha and VEGF, (1, 7) and is known to bind to LFA-1 ( alpha _L beta ₂) on the surface of PBMCs, blocking LFA-1 interaction with ICAM-1 (8). Normal circulating levels of Endocan are approximately 1 ng/mL (6).

References

Lassalle, P. et al. (1996) J. Biol. Chem. 271:20458.
Lassalle, P. (1999) Genbank Accession #: Q9QYY7.
Bechard, D. et al. (2001) J. Biol. Chem. 276:48341.
Aitkenhead, M. et al. (2002) Microvasc. Res. 63:159.
Wellner, M. et al. (2003) Horm. Metab. Res. 35:217
Bechard, D. et al. (2000) J. Vasc. Res. 37:417.
Tsai, J.C. et al. (2002) J. Vasc. Res. 39:148.

Entrez Gene IDs

11082 (Human); 71690 (Mouse)

Alternate Names

Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6

Product Datasheets

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COA

SDS

Related Research Areas

Citations for Mouse Endocan/ESM-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone
Authors: E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim
Experimental & Molecular Medicine, 2023-02-24;55(2):470-484.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Gatekeeping role of Nf2/Merlin in vascular tip EC induction through suppression of VEGFR2 internalization
Authors: JH Bae, MJ Yang, SH Jeong, J Kim, SP Hong, JW Kim, YH Kim, GY Koh
Science Advances, 2022-06-10;8(23):eabn2611.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Limited hyperoxia-induced proliferative retinopathy: A model of persistent retinal vascular dysfunction, preretinal fibrosis and hyaloidal vascular reprogramming for retinal rescue
Authors: T Tedeschi, K Lee, W Zhu, AA Fawzi
PLoS ONE, 2022-04-27;17(4):e0267576.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Lack of WWC2 Protein Leads to Aberrant Angiogenesis in Postnatal Mice
Authors: VC Brücher, C Egbring, T Plagemann, PI Nedvetsky, V Höffken, H Pavenstädt, N Eter, J Kremerskot, P Heiduschka
International Journal of Molecular Sciences, 2021-05-18;22(10):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis
Authors: TS Malinova, A Angulo-Ura, J Nüchel, M Tauber, MM van der St, V Janssen, A de Haan, AG Groenen, M Tebbens, M Graupera, M Plomann, S Huveneers
Nature Communications, 2021-05-10;12(1):2610.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs
Authors: R Ichijo, M Kabata, H Kidoya, F Muramatsu, R Ishibashi, K Abe, K Tsutsui, H Kubo, Y Iizuka, S Kitano, H Miyachi, Y Kubota, H Fujiwara, A Sada, T Yamamoto, F Toyoshima
Science Advances, 2021-02-10;7(7):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Decreased expression of endothelial cell specific molecule-1 in lung tissue in emphysematous mice and stable COPD patients
Authors: Y Zhang, P Chen, S Cai, J Li, Y Chen
Iranian journal of basic medical sciences, 2020-12-01;23(12):1610-1617.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot
Endocan Blockade Suppresses Experimental Ocular Neovascularization in Mice
Authors: T Su, Y Zhong, AM Demetriade, J Shen, A Sui, Y Yao, Y Gao, Y Zhu, X Shen, B Xie
Invest. Ophthalmol. Vis. Sci., 2018-02-01;59(2):930-939.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-Fr, Western Blot
Integrin beta1 controls VE-cadherin localization and blood vessel stability.
Authors: Yamamoto H, Ehling M, Kato K, Kanai K, van Lessen M, Frye M, Zeuschner D, Nakayama M, Vestweber D, Adams R
Nat Commun, 2015-03-10;6(0):6429.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
Transcriptional profiling of VEGF-A and VEGF-C target genes in lymphatic endothelium reveals endothelial-specific molecule-1 as a novel mediator of lymphangiogenesis.
Authors: Shin JW, Huggenberger R, Detmar M
Blood, 2008-07-09;112(6):2318-26.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr