Mouse EOMES Alexa Fluor® 488-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC8889G-025
Detection  of EOMES in Mouse Splenoctyes by Flow Cytometry.
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Mouse EOMES Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EOMES in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1219A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse EOMES
Met1-Ser126
Accession # O54839
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of EOMES in Mouse Splenoctyes by Flow Cytometry. View Larger

Detection of EOMES in Mouse Splenoctyes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse EOMES Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC8889G) or (B) Normal Rabbit IgG Alexa Fluor 488 Control (Catalog # IC105G).  To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).  View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: EOMES

EOMES (Eomesodermin), also TBR2, is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Mouse EOMES is 707 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 278-458). There is one isoform that shows a deletion of aa 463-481. Over aa 1‑126, mouse EOMES shares 76% aa sequence identity with human EOMES.

Long Name
Eomesodermin Homolog
Entrez Gene IDs
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2

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