Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Flow Cytometry

Cited:

Immunohistochemistry, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1219A
View all EOMES Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse EOMES
Met1-Ser126
Accession # O54839

Specificity

Detects mouse EOMES in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse EOMES Antibody

Detection of EOMES antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of EOMES in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC-conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse EOMES Monoclonal Antibody (Catalog # MAB8889) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Applications for Mouse EOMES Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse splenocytes

Reviewed Applications

Read 1 review rated 5 using MAB8889 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EOMES

EOMES (Eomesodermin), also TBR2, is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.

Long Name

Eomesodermin Homolog

Alternate Names

Eomesodermin, TBR2

Entrez Gene IDs

8320 (Human); 13813 (Mouse); 316052 (Rat)

Gene Symbol

EOMES

UniProt

O54839

Additional EOMES Products

Product Documents for Mouse EOMES Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EOMES Antibody

For research use only

Citations for Mouse EOMES Antibody

Customer Reviews for Mouse EOMES Antibody (1)

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Customer Images

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  • Mouse EOMES Antibody
    Name: Efstathios Stamatiades
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Salivary gland tissue
    Species: Mouse
    Verified Customer | Posted 07/09/2019
    Cryosections from PLP immersion fixed tissues were stained for EOMES (1:500) for 4h @ RT. After washing, tissue was stained with Goat anti-Rabbit alexa555 2ndary antibody (1:500) for 1h at RT. CD45 in green, EOMES in red, nuclear staining (Hoechst) in blue.
    Mouse EOMES Antibody MAB8889

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