Mouse EOMES Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB8889
MAB8889-SP
Detection of EOMES in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (1)
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Mouse EOMES Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EOMES in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1219A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse EOMES
Met1-Ser126
Accession # O54839
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of EOMES in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of EOMES in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse EOMES Monoclonal Antibody (Catalog # MAB8889) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: EOMES

EOMES (Eomesodermin), also TBR2, is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.

Long Name
Eomesodermin Homolog
Entrez Gene IDs
8320 (Human); 13813 (Mouse); 316052 (Rat)
Alternate Names
EOMES; eomesodermin (Xenopus laevis) homolog; Eomesodermin; t box, brain, 2; T-box brain protein 2; T-box brain2; TBR2; TBR-2; TBR2eomesodermin homolog; T-brain-2

Product Datasheets

Citation for Mouse EOMES Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Identification of dynamic undifferentiated cell states within the male germline
    Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
    Nat Commun, 2018;9(1):2819.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F

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