Mouse Epigen Antibody

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Product Details
Citations (1)
Supplemental Products

Mouse Epigen Antibody Summary

Species Reactivity
Detects mouse Epigen in ELISAs.
Monoclonal Rat IgG2A Clone # 187204
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Epigen
Accession # Q924X1.1
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration

Mouse Epigen Sandwich Immunoassay

Recommended Concentration
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Epigen Biotinylated Antibody (Catalog # BAF1127)

Standard: Recombinant Mouse Epigen Protein (Catalog # 1127-EP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Epigen

Epigen (EPGN) is an epithelial mitogen that belongs to the EGF superfamily (1-3). Mouse Epigen cDNA encodes a 152 amino acid (aa) residue type I transmembrane precursor protein with a 23 aa putative signal peptide, an 86 aa extracellular domain, a 21 aa transmembrane domain and a 22 aa cytoplasmic domain (2, 3). The extracellular domain (aa 24-109) contains a pattern of six cysteines typical of EGF family proteins (1-3). Epigen is among several EGF family proteins that can undergo metalloproteinase cleavage to generate a soluble form (3, 4). Cleavage of Epigen by TACE/ADAM17 has been demonstrated (4). The mature, shed form of mouse Epigen (aa 53-103) shares 92%, 96% and 90% aa sequence identity with human, rat and equine Epigen, respectively, and more than 40% aa identity with corresponding regions of TGF-alpha and epiregulin (2). Epigen mRNA is found in many tissues, but it is mainly expressed in actively proliferating cells within these tissues. For example, Epigen in skin is found mainly in the proliferating root sheath of hair follicles, and transgenic overexpression in the skin causes hyperplasia in sebaceous glands (3, 5). Epigen is also expressed developmentally and by invasive adenocarcinomas of the breast and prostate (3). Epigen is strongly mitogenic for epithelial cells and fibroblasts, despite its relatively weak affinity for its main receptor, ErbB1 (2, 3). The mitogenic potential of Epigen is enhanced by its unusually long persistence on the membrane before ubiquitylation and receptor-mediated depletion (3).

  1. Schneider, M.R. and E. Wolf (2010) J. Cell. Physiol. 218:460.
  2. Strachan, L. et al. (2001) J. Biol. Chem. 276:18265.
  3. Kochupurakkal, B.S. et al. (2005) J. Biol. Chem. 280:8503.
  4. Sahin, U. and C.P. Blobel 2007) FEBS Lett. 581:41.
  5. Dahlhoff, M. et al. (2010) J. Invest. Dermatol. 130:623.
Entrez Gene IDs
255324 (Human); 71920 (Mouse)
Alternate Names
ALGV3072; EPG; EPGN; Epigen; PRO9904

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Product Specific Notices

U.S. Patent No. 6,150,502 used under license from Genesis Research and Development Corporation Limited

Citation for Mouse Epigen Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Epiregulin, epigen and betacellulin antibodies and axial elongation in young guinea pigs with lens-induced myopization
    Authors: L Dong, RH Zhang, WD Zhou, YF Li, HY Li, HT Wu, XH Shi, JB Jonas, WB Wei
    BMC ophthalmology, 2022-04-27;22(1):193.
    Species: Guinea Pig
    Sample Types: In Vivo
    Applications: In Vivo


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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