Detection of Mouse ETV2/ER71 by Western Blot. Western blot shows lysates of bEnd.3 mouse endothelioma cell line and D3 mouse embryonic stem cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse ETV2/ER71 Monoclonal Antibody (Catalog # MAB7740) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ETV2/ER71 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
ETS translocation variant 2 (ETV2), also
known as ETSRP and ER71, is a 37 kDa transcription factor that is expressed in
presumptive vascular progenitor cells. It plays an important role in vascular
development by promoting the expansion of hemangioblasts and vascular
endothelial cells while inhibiting differentiation into cardiomyocytes. It
contains one ETS DNA binding domain (aa 234-314). Within aa 125-231, mouse ETV2
shares 54% and 85% aa sequence identity with human and rat ETV2, respectively.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.