Mouse FCRL1/FcRH1 Antibody

Catalog # Availability Size / Price Qty
AF7286
AF7286-SP
Detection of FcRL1/FcRH1 in Mouse Splenocytes by Flow Cytometry.
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Mouse FCRL1/FcRH1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FCRL1/FcRH1 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant human FCRL1/FcRH1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse FCRL1/FcRH1
Ala17-Ser219
Accession # Q8R4Y0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of FcRL1/FcRH1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of FcRL1/FcRH1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse FCRL1/FcRH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7286) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (Catalog # FAB1217P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRL1/FcRH1

FCRL1 (Fc receptor-like protein 1; also CD307a, FcRH1 and IFGP1) is a 36 kDa (predicted) member of the Ig Superfamily. In mouse, it is found on mature follicular and marginal B cells, splenic T cells and NK cells. FCRL1 is likely not a receptor for immunoglobulin. At present, it would appear that mouse FCRL1 serves as an inhibiting coreceptor, in as much as it contains a series of cytoplasmic ITIM motifs. Mature mouse FCRL1 is a 327 amino acid (aa) type I transmembrane protein (aa 17-343). It contains a 203 aa extracellular region (aa 17-219) that shows two C2-type Ig-like domains (aa 17-200), and a 103 aa cytoplasmic domain. There are multiple potential splice variants. One shows a deletion of aa 206-248 (potentially soluble), a second contains a six aa substitution for aa 319-343, a third possesses a three aa substitution for aa 209-343, and a fourth shows a 21 aa substitution for Ala11. Over aa 17-219, mouse FCRL1 shares 83% aa sequence identity with rat FCRL1. Over the same region, it shares 63% aa sequence identity with human FCRL1 when a third Ig-like domain that is present in human is excluded from the analysis.

Long Name
Fc Receptor-like 1
Entrez Gene IDs
115350 (Human); 229499 (Mouse); 680665 (Rat)
Alternate Names
BXMH1; CD307a antigen; CD307a; Fc receptor homolog 1; Fc receptor-like 1; FcRH1; FCRH1DKFZp667O1421; FCRL1; FcR-like protein 1; hIFGP1; IFGP family protein 1; IFGP1; IFGP1Fc receptor-like protein 1; Immune receptor translocation-associated protein 5; IRTA5; IRTA5immunoglobulin superfamily Fc receptor, gp42

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