Mouse FCRN Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse FCRN in direct ELISAs and Western blots. In direct ELISAs, approximately 6% cross-reactivity with recombinant human FCRN is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived mouse FCRN
    Ser22-Ser301
    Accession # AAH03786
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse FCRN by Western Blot. Western blot shows lysates of mouse liver tissue, mouse small intestine tissue, and mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for FCRN at approximately 48 to 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Intracellular Staining by Flow Cytometry
Detection of FCRN in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FCRN

FcRn (IgG receptor FcRn large subunit p51/ alpha -chain) is a 44-52 kDa member of the Ig superfamily. In mouse, it is expressed by intestinal epithelium of neonates and fetal yolk sac endoderm during pregnancy. Together with p14/ beta 2-Microglobulin, FcRn forms an MHC I-like noncovalent heterodimer termed the FcRn complex. In rodent, FcRn transports IgG in milk into the intestine, and IgG from the mother's blood into the mouse via endodermal FcRn linked to a special Fc gamma RIIb macrophage receptor. It is also expressed by select macrophages and DCs, and participates in endosomal-associated antigen presentation to CD4+ T cells. Mature mouse FcRn is a 344 amino acid (aa) type I transmembrane glycoprotein. It contains a 276 aa extracellular region (aa 22-297) that contains an Ig-like domain (aa 201-288). The FcRn complex forms a homodimer when binding IgG. One potential splice variant shows a four aa insertion after Gly109. Over aa 22-301 of the splice variant (GenBank #:AAH03786), mouse FcRn shares 91% and 68% aa identity with rat and human FcRn, respectively.

  • Long Name:
    Neonatal Fc Receptor
  • Entrez Gene IDs:
    2217 (Human); 14132 (Mouse); 29558 (Rat)
  • Alternate Names:
    alpha-chain; Fc fragment of IgG, receptor, transporter, alpha; FCGRT; FcRn alpha chain; FCRN; FCRNimmunoglobulin receptor, intestinal, heavy chain; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; major histocompatibility complex class I-like Fc receptor; Neonatal Fc receptor; neonatal Fc-receptor for Ig
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. The Neonatal Fc Receptor and Complement Fixation Facilitate Prophylactic Vaccine-Mediated Humoral Protection against Viral Infection in the Ocular Mucosa
    Authors: DJ Royer, MM Carr, HR Gurung, WP Halford, DJJ Carr
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells
    Nat Commun, 2016;7(0):12175.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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