Mouse FCRN Antibody

Catalog # Availability Size / Price Qty
AF6775
AF6775-SP
Detection of Mouse FCRN by Western Blot.
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Product Details
Citations (6)
FAQs
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Mouse FCRN Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse FCRN in direct ELISAs and Western blots. In direct ELISAs, approximately 6% cross-reactivity with recombinant human FCRN is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived mouse FCRN
Ser22-Ser301
Accession # AAH03786
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse FCRN antibody by Western Blot. View Larger

Detection of Mouse FCRN by Western Blot. Western blot shows lysates of mouse liver tissue, mouse small intestine tissue, and mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for FCRN at approximately 48 to 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Intracellular Staining by Flow Cytometry Detection of FCRN antibody in J774A.1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of FCRN in J774A.1 Mouse Cell Line by Flow Cytometry. J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Goat Anti-Mouse FCRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6775, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FCRN

FcRn (IgG receptor FcRn large subunit p51/ alpha -chain) is a 44-52 kDa member of the Ig superfamily. In mouse, it is expressed by intestinal epithelium of neonates and fetal yolk sac endoderm during pregnancy. Together with p14/ beta 2-Microglobulin, FcRn forms an MHC I-like noncovalent heterodimer termed the FcRn complex. In rodent, FcRn transports IgG in milk into the intestine, and IgG from the mother's blood into the mouse via endodermal FcRn linked to a special Fc gamma RIIb macrophage receptor. It is also expressed by select macrophages and DCs, and participates in endosomal-associated antigen presentation to CD4+ T cells. Mature mouse FcRn is a 344 amino acid (aa) type I transmembrane glycoprotein. It contains a 276 aa extracellular region (aa 22-297) that contains an Ig-like domain (aa 201-288). The FcRn complex forms a homodimer when binding IgG. One potential splice variant shows a four aa insertion after Gly109. Over aa 22-301 of the splice variant (GenBank #:AAH03786), mouse FcRn shares 91% and 68% aa identity with rat and human FcRn, respectively.

Long Name
Neonatal Fc Receptor
Entrez Gene IDs
2217 (Human); 14132 (Mouse); 29558 (Rat); 102128913 (Cynomolgus Monkey)
Alternate Names
alpha-chain; Fc fragment of IgG, receptor, transporter, alpha; FCGRT; FcRn alpha chain; FCRN; FCRNimmunoglobulin receptor, intestinal, heavy chain; IgG Fc fragment receptor transporter alpha chain; IgG receptor FcRn large subunit p51; major histocompatibility complex class I-like Fc receptor; Neonatal Fc receptor; neonatal Fc-receptor for Ig

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Citations for Mouse FCRN Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Megalin Orchestrates FcRn Endocytosis and Trafficking
    Authors: E Dahlke, Y Anan, LM Klie, AE Hartkopf, F Theilig
    Cells, 2022-12-22;12(1):.
    Species: Mouse, Opossum
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  2. A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension
    Authors: ND Arnold, JA Pickworth, LE West, S Dawson, JA Carvalho, H Casbolt, AT Braithwait, J Iremonger, L Renshall, V Germaschew, M McCourt, P Bland-Ward, H Kowash, AG Hameed, AMK Rothman, MG Frid, AA Roger Thom, HR Evans, M Southwood, NW Morrell, DC Crossman, MKB Whyte, KR Stenmark, CM Newman, DG Kiely, SE Francis, A Lawrie
    Nat Commun, 2019-11-15;10(1):5183.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization Control
  3. Abl family tyrosine kinases govern IgG extravasation in the skin in a murine pemphigus model
    Authors: S Ono, G Egawa, T Nomura, A Kitoh, T Dainichi, A Otsuka, S Nakajima, M Amagai, F Matsumoto, M Yamamoto, Y Kubota, T Takai, T Honda, K Kabashima
    Nat Commun, 2019-09-30;10(1):4432.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The Neonatal Fc Receptor and Complement Fixation Facilitate Prophylactic Vaccine-Mediated Humoral Protection against Viral Infection in the Ocular Mucosa
    Authors: DJ Royer, MM Carr, HR Gurung, WP Halford, DJJ Carr
    J. Immunol., 2017-07-31;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  5. Distribution of FcRn Across Species and Tissues
    Authors: S Latvala, B Jacobsen, MB Otteneder, A Herrmann, S Kronenberg
    J. Histochem. Cytochem., 2017-04-12;0(0):2215541770509.
    Species: Human, Mouse, Primate, Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells
    Nat Commun, 2016-07-18;7(0):12175.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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