Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Bovine

Applications

Validated:

Western Blot, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 133706
View all FGFR2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FGF R2 alpha (IIIc)
Extracellular domains

Specificity

Detects IIIc isoforms of mouse FGF R2 in direct ELISAs and Western blots. Does not react with IIIb isoforms of recombinant mouse (rm) FGF R2. In direct ELISAs and Western blots, 100% cross-reactivity with IIIc isoforms of recombinant human (rh) FGF R2 is observed, and no cross-reactivity with IIIb isoforms of rhFGF R2 or any isoforms of rhFGF R1, rmFGF R3, or rhFGF R4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse FGFR2 (IIIc) Antibody

FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Rat Anti-Mouse FGF R2 Antibody.

FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Rat Anti-Mouse FGF R2 Antibody.

Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera (Catalog # 716-MF) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R2 (IIIc) Monoclonal Antibody (Catalog # MAB716). The ND50 is typically 0.1-0.4 µg/mL in the presence of heparin (10 µg/mL).
Detection of Mouse FGFR2 (IIIc) by Immunohistochemistry

Detection of Mouse FGFR2 (IIIc) by Immunohistochemistry

FGFRs and PDGFRs in pericyte recruitment. A RT-PCR analysis of mRNA expression levels of FGF receptors in pericytes freshly isolated from T241-vector and T241–FGF-2 tumors using magnetic bead separation. Beta-actin serves as a control. b CD31+ endothelial (red) and NG2+ pericyte (green) signals in vehicle (VT)-, anti-FGFR1 neutralizing antibody-, anti-FGFR2 neutralizing antibody treated-, anti-FGFR3 neutralizing antibody-, BGJ398-, anti-PDGFR alpha neutralizing antibody-, anti-PDGFR beta neutralizing antibody-, and imatinib-treated FGF-2+ tumors. Arrowheads indicate pericyte-associated vessels. Images are presented using whole mount staining. Bar = 50 μm. c, d Quantification of NG2+ pericyte area versus the total CD31+ microvessels and vascular coverage. (n = 7 random fields; n = 4 mice for each group). All data as means ± S.E.M.; Student’s t test, *P < 0.05, **P < 0.01 and ***P < 0.001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29423271), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse FGFR2 (IIIc) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample:

Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera (Catalog # 716-MF)
under non-reducing conditions only

**Not recommended for detection of endogenous levels of Mouse FGF R2 (IIIc) in Western blot

Neutralization

Measured by its ability to neutralize FGF R2 beta -mediated inhibition of proliferation in the NR6R‑3T3 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 3 ng/mL Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera, 0.3 ng/mL Recombinant Human FGF acidic, and 10 µg/mL heparin.

Reviewed Applications

Read 1 review rated 1 using MAB716 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR2

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1‑4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform. Only the alpha  isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1‑3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1‑3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

Long Name

Fibroblast Growth Factor Receptor 2

Alternate Names

CD332, FGF R2

Entrez Gene IDs

2263 (Human); 14183 (Mouse)

Gene Symbol

FGFR2

Additional FGFR2 Products

Product Documents for Mouse FGFR2 (IIIc) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FGFR2 (IIIc) Antibody

For research use only

Citations for Mouse FGFR2 (IIIc) Antibody

Customer Reviews for Mouse FGFR2 (IIIc) Antibody (1)

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  • Mouse FGFR2 (IIIc) Antibody
    Name: Emily Thompson
    Application: Western Blot
    Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line
    Species: Mouse
    Verified Customer | Posted 03/20/2019
    Mouse FGFR2 (IIIc) Antibody MAB716
    Bio-Techne Response
    Thank you for reviewing our product. This antibody was validated using recombinant FGFR2 (IIIc) under non-reducing conditions and our follow-up testing showed that the antibody still works in western blot with this sample. However, in new testing, we were unable to detect endogenous levels of Mouse FGF R2 (IIIc) in Western blot under reducing and non-reducing conditions. We have changed the product insert to reflect this.

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