Mouse FGFR2 (IIIc) Antibody Summary
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Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera (Catalog # 716-MF)
under non-reducing conditions only
**Not recommended for detection of endogenous levels of Mouse FGF R2 (IIIc) in Western blot
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
FGF R2 beta Inhibition of FGF acidic-dependent Cell Proliferation and Neutralization by Rat Anti-Mouse FGF R2 Antibody. Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera (Catalog # 716-MF) inhibits Recombinant Human FGF acidic (Catalog # 232-FA) induced proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human FGF acidic (0.3 ng/mL) activity elicited by Recombinant Mouse FGF R2 beta (IIIc) Fc Chimera (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse FGF R2 (IIIc) Monoclonal Antibody (Catalog # MAB716). The ND50 is typically 0.1-0.4 µg/mL in the presence of heparin (10 µg/mL).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorigenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1‑4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid‑box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1‑3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha isoform, or only IgII and IgIII, referred to as the beta isoform. Only the alpha isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1‑3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1‑3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.
Citations for Mouse FGFR2 (IIIc) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Expression and functional role of fibroblast growth factors (FGF) in placenta during different stages of pregnancy in water buffalo (Bubalus bubalis)
Authors: HL Devi, S Kumar, YY Konyak, J Bharati, A Bhimte, Y Pandey, K Kumar, A Paul, A Kala, HA Samad, MR Verma, G Singh, S Bag, M Sarkar, VS Chouhan
Sample Types: Whole Tissue
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2018;15(1):39-46.
Sample Types: Whole Tissue
Treatment with FGFR2-IIIc monoclonal antibody suppresses weight gain and adiposity in KKA(y) mice
Authors: K Nonogaki
Nutr Diabetes, 2016;6(11):e233.
FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc.
Authors: Nakayama T, Mutsuga N, Tosato G
Sample Types: Whole Cells
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***Bio-Techne Response: Thank you for reviewing our product. This antibody was validated using recombinant FGFR2 (IIIc) under non-reducing conditions and our follow-up testing showed that the antibody still works in western blot with this sample. However, in new testing, we were unable to detect endogenous levels of Mouse FGF R2 (IIIc) in Western blot under reducing and non-reducing conditions. We have changed the product insert to reflect this.***