Detection of Flt‑3/Flk‑2 in M1 Mouse Cell Line by Flow Cytometry.
M1 mouse myeloid leukemia cell line was stained with Rat Anti-Mouse Flt‑3/Flk‑2 PE‑conjugated Monoclonal Antibody (Catalog # FAB7681P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (Fetal Liver Kinase) and Stk-1 (Stem cell Tyrosine Kinase) and designated CD135, is a 150 kDa member of the class III subfamily of receptor tyrosine kinases. This familay includes KIT, the receptor for SCF, and C-FMS, the receptor for M-CSF. The extracellular region of these receptors contains five immunoglobulin-like domains and the intracellular region contains a split kinase domain. Mouse Flt-3 cDNA encodes a 992 amino acid (aa) residue type I membrane protein with a 27 aa residue signal peptide, a 517 aa extracellular domain with 10 potential N-linked glycosylation sites, a 20 aa residue transmembrane domain and a 428 aa residue cytoplasmic domain. Mouse Flt-3 shares 85% amino acid sequence identity with human Flt-3. Flt-3 expression has been detected on neurons and hematopoietic progenitor cell populations. The ligand for Flt-3 (FL) has been identified to be a transmembrane protein with structural homology to M-CSF and SCF. Recombinant soluble Flt-3 Fc chimeric protein has been shown to bind FL with high affinity and is a potent FL antagonist. Over aa 28-544, mouse Flt-3 shares 89% and 84% aa sequence identity with rat and human Flt-3, respectively.
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