Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human

Applications

Validated:

Western Blot

Cited:

ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 210725
View all Follistatin-related Gene Protein/FLRG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse FLRG
Val24-Val256
Accession # Q9EQC7

Specificity

Detects mouse FLRG in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows 50-100% cross-reactivity with recombinant human FLRG.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse Follistatin-related Gene Protein/FLRG Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse Follistatin-related Gene Protein/FLRG (Catalog # 1255-F3)

Reviewed Applications

Read 1 review rated 5 using MAB1255 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Follistatin-related Gene Protein/FLRG

Follistatin-Related Gene Protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Mouse FLRG cDNA encodes a 256 amino acid (aa) residue protein with a putative 23 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C‑terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7, and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293, and CHO cells (1-5).

References

  1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.
  2. Sidis, Y. et al. (2002) Endocrinology 143:1613.
  3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.
  4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.
  5. Bartholin, L. et al. (2001) Oncogene 20:5409.

Alternate Names

Follistatin-like 3, FSTL3

Entrez Gene IDs

10272 (Human); 83554 (Mouse)

Gene Symbol

FSTL3

UniProt

Q9EQC7

Additional Follistatin-related Gene Protein/FLRG Products

Product Documents for Mouse Follistatin-related Gene Protein/FLRG Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Follistatin-related Gene Protein/FLRG Antibody

For research use only

Related Research Areas

Activin Family
BMP Family

Citations for Mouse Follistatin-related Gene Protein/FLRG Antibody

Customer Reviews for Mouse Follistatin-related Gene Protein/FLRG Antibody (1)

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  • Mouse Follistatin-related Gene Protein/FLRG Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    Mouse Follistatin-related Gene Protein/FLRG Antibody MAB1255

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