Mouse FOLR4 Antibody (721601)

R&D Systems | Catalog # MAB6124

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 721601
View all FOLR4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FOLR4
Gly20-Ser100
Accession # Q9EQF4

Specificity

Detects mouse FOLR4 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) FOLR1, rmFOLR2, recombinant human (rh) FOLR3, or rhFOLR4 is observed. In Western blots, no cross-reactivity with rhFOLR1, 2, or 3 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse FOLR4 Antibody (721601)

Detection of Mouse FOLR4 antibody by Western Blot.

Detection of Mouse FOLR4 by Western Blot.

Western blot shows lysates of mouse CD4+CD25-T cells and mouse CD4+CD25+T cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse FOLR4 Monoclonal Antibody (Catalog # MAB6124) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for FOLR4 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Mouse FOLR4 Antibody (721601)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Mouse CD4+CD25- T cells and mouse CD4+CD25+ T cells

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FOLR4

FOLR4 (folate receptor 4; also FR4, FR-d and FOLBP-3) is a 35 kDa glycoprotein member of the folate receptor family. It is found on mouse Treg cells, particularly those classified as natural, and CD4+ CD25- T cells that produce low amounts of IFN-gamma. Although folate family receptors can internalize folate, they are generally not the principal conduits for folate uptake. Mouse FOLR4 preproprecursor is 244 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 225 aa proform. While there are no definitive structural motifs, there is presumably a C-terminal propeptide that gives rise to a GPI-linkage. There are three potential isoforms. One is 37 kDa in size and shows a 36 aa insertion after Glu155. Two others show a deletion of  aa 47-109, and a 17 aa substitution for aa 157-244. Over aa 20-100, mouse FOLR4 shares 84% and 70% aa identity with rat and human FOLR4, respectively.

Long Name

Folate Receptor 4

Alternate Names

FR-delta, LOC390243

Entrez Gene IDs

390243 (Human); 64931 (Mouse); 690037 (Rat)

Gene Symbol

IZUMO1R

UniProt

Q9EQF4

Additional FOLR4 Products

Product Documents for Mouse FOLR4 Antibody (721601)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse FOLR4 Antibody (721601)

For research use only

Related Research Areas

Cancer Biomarkers

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