Mouse Frizzled-7 Antibody

Catalog # Availability Size / Price Qty
MAB198-050
MAB198-500
MAB198-SP
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Mouse Frizzled-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects recombinant mouse (rm) Frizzled-7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity  with recombinant mouse Frizzled-1, -2, -3, -4, -6 or -8 is observed.
Source
Monoclonal Rat IgG1 Clone # 151107
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185 (predicted)
Accession # Q61090
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Frizzled‑7 Fc Chimera (Catalog # 198-FZ)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1). Members of the Frizzled family of proteins serve as receptors for the Wnt signaling pathway (2). The predicted structure of Frizzled proteins is similar among all family members, containing a divergent N-terminal signal peptide, a highly conserved extracellular cysteine-rich domain (CRD), a variable-length linker region, a seven-pass transmembrane region, and a variable-length C-terminal cytoplasmic domain. The CRD, which comprises the Wnt binding site, spans about 130 amino acid residues and contains ten invariant cysteine residues. Mouse Frizzled-7 contains 572 amino acid residues and shares 99% identity with the human orthologue in the CRD (3, 4). Frizzled-7 mRNA has been detected in relatively large amounts in adult skeletal muscle, plancenta and heart, and fetal kidney and lung (3). Several Frizzled-dependent signaling pathways exist (2). Their activation depends on the Wnt ligand and the cell context. Frizzled-7 can activate canonical Wnt/ beta -Catenin signaling, as well as a non-canonical pathway involved in tissue morphogenesis (4, 5).

References
  1. Wodarz, A. and R. Nusse (1998) Annu. Rev. Cell Dev. Biol. 14:59.
  2. Hsieh, J.-C. (2004) Front. Biosci. 9:1333.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Sheldahl, L.C. et al. (1999) Curr. Biol. 9:695.
  5. Medina, A. et al. (2000) Mech. Dev. 92:227.
Entrez Gene IDs
8324 (Human); 14369 (Mouse)
Alternate Names
frizzled (Drosophila) homolog 7; frizzled homolog 7 (Drosophila); Frizzled, drosophila, homolog of, 7; Frizzled7; Frizzled-7; Fz-7; FZD7; FzE3frizzled-7; hFz7

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