Mouse G-CSF Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF414
Product Details
Citations (6)
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Mouse G-CSF Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse G-CSF in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) G‑CSF, rhCNTF, recombinant mouse (rm) IL‑6, rmLIF, rmOSM, rmCT‑1, rmLeptin, and rmIL‑11 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse G-CSF (R&D Systems, Catalog # 414-CS)
Val31-Ala208
Accession # P09920
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse G-CSF (Catalog # 414-CS)

Mouse G-CSF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse G‑CSF Antibody (Catalog # MAB414)

Standard: Recombinant Mouse G-CSF Protein (Catalog # 414-CS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1 or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin

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Citations for Mouse G-CSF Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. RIPK3 promotes cell death and NLRP3 inflammasome activation in the absence of MLKL.
    Authors: Lawlor K, Khan N, Mildenhall A, Gerlic M, Croker B, D'Cruz A, Hall C, Kaur Spall S, Anderton H, Masters S, Rashidi M, Wicks I, Alexander W, Mitsuuchi Y, Benetatos C, Condon S, Wong W, Silke J, Vaux D, Vince J
    Nat Commun, 2015-02-18;6(0):6282.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  2. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  3. Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
    Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
    PLoS ONE, 2011-05-31;6(5):e19957.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  4. Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
    Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
    J. Exp. Med., 2001-08-20;194(4):519-27.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  5. Longitudinal Multiplexed Measurement of Quantitative Proteomic Signatures in Mouse Lymphoma Models Using Magneto-Nanosensors
    Authors: JR Lee, I Appelmann, C Miething, TO Shultz, D Ruderman, D Kim, P Mallick, SW Lowe, SX Wang
    Theranostics, 2018-02-03;8(5):1389-1398.
  6. Experimental and theoretical investigation of the precise transduction mechanism in giant magnetoresistive biosensors
    Authors: Jung-Rok Lee, Noriyuki Sato, Daniel J. B. Bechstein, Sebastian J. Osterfeld, Junyi Wang, Adi Wijaya Gani et al.
    Scientific Reports

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