Mouse G-CSF Antibody

Catalog # Availability Size / Price Qty
MAB414-SP
MAB414-100
MAB414-500
Mouse G-CSF Antibody in Data
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Product Details
Citations (30)
FAQs
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Mouse G-CSF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse G-CSF in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) G‑CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.
Source
Monoclonal Rat IgG1 Clone # 67604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse G-CSF
Val31-Ala208
Accession # P09920
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse G-CSF (Catalog # 414-CS)
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Mouse G-CSF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse G‑CSF Biotinylated Antibody (Catalog # BAF414)

Standard: Recombinant Mouse G-CSF Protein (Catalog # 414-CS)

Neutralization
Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cells line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of G‑CSF in Mouse splenocytes by Flow Cytometry. View Larger

Detection of G‑CSF in Mouse splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS (100 µg/ml) for 24 hours were stained with Rat Anti-Mouse G-CSF Monoclonal Antibody (Catalog # MAB414, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by Anti-Rat IgG PE-conjugated secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Neutralization Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody. View Larger

Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody. Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G-CSF Monoclonal Antibody (Catalog # MAB414). The ND50is typically 0.01-0.03 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Long Name
Granulocyte Colony Stimulating Factor
Entrez Gene IDs
1440 (Human); 12985 (Mouse)
Alternate Names
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; Pluripoietin

Product Datasheets

Citations for Mouse G-CSF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer
    Authors: JM Ubellacker, N Baryawno, N Severe, MJ DeCristo, J Sceneay, JN Hutchinson, MT Haider, CS Rhee, Y Qin, WM Gregory, AC Garrido-Ca, I Holen, JE Brown, RE Coleman, DT Scadden, SS McAllister
    Cancer Res., 2018;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance
    Authors: MA Meyer, JM Baer, BL Knolhoff, TM Nywening, RZ Panni, X Su, KN Weilbaeche, WG Hawkins, C Ma, RC Fields, DC Linehan, GA Challen, R Faccio, RL Aft, DG DeNardo
    Nat Commun, 2018;9(1):1250.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  3. Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection
    Authors: F Sparber, T Dolowschia, S Mertens, L Lauener, BE Clausen, N Joller, P Stoitzner, R Tussiwand, S LeibundGut
    PLoS Pathog., 2018;14(5):e1007069.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  4. Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils
    Authors: J Banuelos, Y Cao, SC Shin, BS Bochner, P Avila, S Li, X Jiang, MW Lingen, RP Schleimer, NZ Lu
    PLoS ONE, 2017;12(5):e0177884.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  5. Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis
    Authors: H Zhu, HJ Kwak, P Liu, B Bajrami, Y Xu, SY Park, C Nombela-Ar, S Mondal, H Kambara, H Yu, L Chai, LE Silberstei, T Cheng, HR Luo
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis
    Authors: Arna E Andrews
    J. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  7. Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
    Authors: Bohannon J, Luan L, Hernandez A, Afzal A, Guo Y, Patil N, Fensterheim B, Sherwood E
    J Leukoc Biol, 2016;99(4):629-40.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  9. Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) Ly6C(+) monocytes that are poised to release TNF-alpha.
    Authors: Sivakumaran S, Henderson S, Ward S, Santos E Sousa P, Manzo T, Zhang L, Conlan T, Means T, D'Aveni M, Hermine O, Rubio M, Chakraverty R, Bennett C
    Eur J Immunol, 2016;46(1):192-203.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  10. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity
    Cell, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection
    Authors: Anna A Müller
    PLoS Pathog, 2016;12(6):e1005723.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  12. Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
    Authors: Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A
    Cancer Res, 2015;75(13):2653-62.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  13. G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation.
    Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J
    Cancer Res, 2015;75(12):2566-79.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
    Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
    Nature, 2015;522(7556):345-8.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  15. IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils.
    Authors: Trautwein-Weidner K, Gladiator A, Nur S, Diethelm P, LeibundGut-Landmann S
    Mucosal Immunol, 2015;8(2):221-31.
    Species: Mouse
    Sample Types: In Vivo
  16. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
    Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
    Stem Cells, 2015;33(3):848-58.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
    Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
    J Invest Dermatol, 2014;134(5):1295-304.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  18. G-CSF drives a posttraumatic immune program that protects the host from infection.
    Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
    J Immunol, 2014;192(5):2405-17.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  19. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  20. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
    Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
    J. Clin. Invest., 2011;121(11):4446-61.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
    Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
    PLoS ONE, 2011;6(5):e19957.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
    Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
    PLoS ONE, 2011;6(11):e27690.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  23. Cannabinoid receptor activation leads to massive mobilization of myeloid-derived suppressor cells with potent immunosuppressive properties.
    Authors: Hegde VL, Nagarkatti M, Nagarkatti PS
    Eur. J. Immunol., 2010;40(12):3358-71.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  24. G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
    Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  25. The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse.
    Authors: Furze RC, Rankin SM
    FASEB J., 2008;22(9):3111-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  26. Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow.
    Authors: Brunner S, Zaruba MM, Huber B, David R, Vallaster M, Assmann G, Mueller-Hoecker J, Franz WM
    Exp. Hematol., 2008;36(9):1157-66.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  27. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
    Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
    Blood, 2007;111(1):42-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  28. Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
    Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
    Nature, 2007;450(7171):825-31.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  29. Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
    Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
    J. Exp. Med., 2001;194(4):519-27.
    Species: Mouse
    Sample Types: BALF
    Applications: ELISA Development
  30. Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
    Authors: Kulkarni, Shilpa, Singh, Pankaj K, Ghosh, Sanchita, Posarac, Ana, Singh, Vijay K
    Cytokine, 213;62(2):278-85.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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Mouse G-CSF Antibody
By Anonymous on 04/13/2018
Application: IHC Sample Tested: Serum Species: Mouse