Mouse G-CSF Antibody

(26 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse G-CSF in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) G‑CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.
  • Source
    Monoclonal Rat IgG1 Clone # 67604
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse G-CSF
    Val31-Ala208
    Accession # P09920
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse G-CSF (Catalog # 414-CS)
    • Mouse G-CSF Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse G‑CSF Antibody (Catalog # MAB414)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse G‑CSF Biotinylated Antibody (Catalog # BAF414)
  • ELISA Standard
     
    Recombinant Mouse G-CSF Protein (Catalog # 414-CS)
  • Neutralization
    Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cells line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody.

Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the NFS‑60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G‑CSF Monoclonal Antibody (Catalog # MAB414). The ND50 is typically 0.01-0.03 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

  • Long Name:
    Granulocyte Colony Stimulating Factor
  • Entrez Gene IDs:
    1440 (Human); 12985 (Mouse)
  • Alternate Names:
    C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils
    Authors: J Banuelos, Y Cao, SC Shin, BS Bochner, P Avila, S Li, X Jiang, MW Lingen, RP Schleimer, NZ Lu
    PLoS ONE, 2017;12(5):e0177884.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis
    Authors: H Zhu, HJ Kwak, P Liu, B Bajrami, Y Xu, SY Park, C Nombela-Ar, S Mondal, H Kambara, H Yu, L Chai, LE Silberstei, T Cheng, HR Luo
    J. Immunol, 2017;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  3. Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) Ly6C(+) monocytes that are poised to release TNF-alpha.
    Authors: Sivakumaran S, Henderson S, Ward S, Santos E Sousa P, Manzo T, Zhang L, Conlan T, Means T, D'Aveni M, Hermine O, Rubio M, Chakraverty R, Bennett C
    Eur J Immunol, 2016;46(1):192-203.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  4. Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
    Authors: Bohannon J, Luan L, Hernandez A, Afzal A, Guo Y, Patil N, Fensterheim B, Sherwood E
    J Leukoc Biol, 2016;99(4):629-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  5. Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer
    Eur J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  6. An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection
    Authors: Anna A Müller
    PLoS Pathog, 2016;12(6):e1005723.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  7. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity
    Cell, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
    Authors: Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A
    Cancer Res, 2015;75(13):2653-62.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  9. IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
    Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
    Nature, 2015;522(7556):345-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  10. G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation.
    Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J
    Cancer Res, 2015;75(12):2566-79.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  11. Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
    Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
    Stem Cells, 2015;33(3):848-58.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  12. IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils.
    Authors: Trautwein-Weidner K, Gladiator A, Nur S, Diethelm P, LeibundGut-Landmann S
    Mucosal Immunol, 2015;8(2):221-31.
    Species: Mouse
    Sample Type: In Vivo
  13. Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
    Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
    J Invest Dermatol, 2014;134(5):1295-304.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  14. G-CSF drives a posttraumatic immune program that protects the host from infection.
    Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
    J Immunol, 2014;192(5):2405-17.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  15. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012;58(2):169-77.
    Species: Mouse
    Sample Type: Plasma
    Application: Luminex Assay Development
  16. Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
    Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
    J. Clin. Invest., 2011;121(11):4446-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  17. Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
    Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
    PLoS ONE, 2011;6(11):e27690.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
    Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
    PLoS ONE, 2011;6(5):e19957.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  19. Cannabinoid receptor activation leads to massive mobilization of myeloid-derived suppressor cells with potent immunosuppressive properties.
    Authors: Hegde VL, Nagarkatti M, Nagarkatti PS
    Eur. J. Immunol., 2010;40(12):3358-71.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  20. G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
    Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  21. Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow.
    Authors: Brunner S, Zaruba MM, Huber B, David R, Vallaster M, Assmann G, Mueller-Hoecker J, Franz WM
    Exp. Hematol., 2008;36(9):1157-66.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  22. The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse.
    Authors: Furze RC, Rankin SM
    FASEB J., 2008;22(9):3111-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  23. Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
    Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
    Nature, 2007;450(7171):825-31.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  24. The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
    Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
    Blood, 2007;111(1):42-9.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  25. Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
    Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
    J. Exp. Med., 2001;194(4):519-27.
    Species: Mouse
    Sample Type: BALF
    Application: ELISA Development
  26. Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
    Authors: Kulkarni, Shilpa, Singh, Pankaj K, Ghosh, Sanchita, Posarac, Ana, Singh, Vijay K
    Cytokine, 213;62(2):278-85.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
Expand to show all 26 Citations
Isotype Controls
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Rat IgG1 Isotype Control

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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

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Rat IgG1 Isotype Control

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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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