Mouse G-CSF Antibody Summary
Val31-Ala208
Accession # P09920
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Applications
Mouse G-CSF Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data

Detection of G‑CSF in Mouse splenocytes by Flow Cytometry. Mouse splenocytes treated with LPS (100 µg/ml) for 24 hours were stained with Rat Anti-Mouse G-CSF Monoclonal Antibody (Catalog # MAB414, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram) followed by Anti-Rat IgG PE-conjugated secondary antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody. Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the NFS-60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G-CSF Monoclonal Antibody (Catalog # MAB414). The ND50 is typically 0.01-0.03 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: G-CSF
G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.
The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.
In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the Go period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.
Product Datasheets
Citations for Mouse G-CSF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Leukotriene B4 Receptor 2 Mediates the Production of G-CSF That Plays a Critical Role in Steroid-Resistant Neutrophilic Airway Inflammation
Authors: DW Kwak, D Park, JH Kim
Biomedicines, 2022;10(11):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Bone Marrow Harbors a Unique Population of Dendritic Cells with the Potential to Boost Neutrophil Formation upon Exposure to Fungal Antigen
Authors: M Goedhart, E Slot, MF Pascutti, S Geerman, T Rademakers, B Nota, S Huveneers, JDV Buul, KC MacNamara, C Voermans, MA Nolte
Cells, 2021;11(1):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
Liver-specific T regulatory type-1 cells program local neutrophils to suppress hepatic autoimmunity via CRAMP
Authors: CS Umeshappa, P Solé, BGJ Surewaard, J Yamanouchi, S Mohapatra, MM Uddin, R Clarke, M Ortega, S Singha, D Mondal, Y Yang, DAA Vignali, P Serra, P Kubes, P Santamaria
Cell Reports, 2021;34(13):108919.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Granulocyte colony-stimulating factor (G-CSF) mediates bone resorption in periodontitis
Authors: H Yu, T Zhang, H Lu, Q Ma, D Zhao, J Sun, Z Wang
BMC Oral Health, 2021;21(1):299.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Targeting IL-17A/glucocorticoid synergy to CSF3 expression in neutrophilic airway diseases
Authors: S Ouyang, C Liu, J Xiao, X Chen, AC Lui, X Li
JCI Insight, 2020;5(3):.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Langerin+ DCs regulate innate IL-17 production in the oral mucosa during Candida albicans-mediated infection
Authors: F Sparber, T Dolowschia, S Mertens, L Lauener, BE Clausen, N Joller, P Stoitzner, R Tussiwand, S LeibundGut
PLoS Pathog., 2018;14(5):e1007069.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance
Authors: MA Meyer, JM Baer, BL Knolhoff, TM Nywening, RZ Panni, X Su, KN Weilbaeche, WG Hawkins, C Ma, RC Fields, DC Linehan, GA Challen, R Faccio, RL Aft, DG DeNardo
Nat Commun, 2018;9(1):1250.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Modulating bone marrow hematopoietic lineage potential to prevent bone metastasis in breast cancer
Authors: JM Ubellacker, N Baryawno, N Severe, MJ DeCristo, J Sceneay, JN Hutchinson, MT Haider, CS Rhee, Y Qin, WM Gregory, AC Garrido-Ca, I Holen, JE Brown, RE Coleman, DT Scadden, SS McAllister
Cancer Res., 2018;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils
Authors: J Banuelos, Y Cao, SC Shin, BS Bochner, P Avila, S Li, X Jiang, MW Lingen, RP Schleimer, NZ Lu
PLoS ONE, 2017;12(5):e0177884.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis
Authors: H Zhu, HJ Kwak, P Liu, B Bajrami, Y Xu, SY Park, C Nombela-Ar, S Mondal, H Kambara, H Yu, L Chai, LE Silberstei, T Cheng, HR Luo
J. Immunol, 2017;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Therapeutic Targeting of the G-CSF Receptor Reduces Neutrophil Trafficking and Joint Inflammation in Antibody-Mediated Inflammatory Arthritis
Authors: Arna E Andrews
J. Immunol., 2016;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer
Eur J Immunol, 2016;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity
Cell, 2016;0(0):.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection
Authors: Anna A Müller
PLoS Pathog, 2016;12(6):e1005723.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) Ly6C(+) monocytes that are poised to release TNF-alpha.
Authors: Sivakumaran S, Henderson S, Ward S, Santos E Sousa P, Manzo T, Zhang L, Conlan T, Means T, D'Aveni M, Hermine O, Rubio M, Chakraverty R, Bennett C
Eur J Immunol, 2016;46(1):192-203.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury.
Authors: Bohannon J, Luan L, Hernandez A, Afzal A, Guo Y, Patil N, Fensterheim B, Sherwood E
J Leukoc Biol, 2016;99(4):629-40.
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo -
IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils.
Authors: Trautwein-Weidner K, Gladiator A, Nur S, Diethelm P, LeibundGut-Landmann S
Mucosal Immunol, 2015;8(2):221-31.
Species: Mouse
Sample Types: In Vivo
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IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis.
Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K
Nature, 2015;522(7556):345-8.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation.
Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J
Cancer Res, 2015;75(12):2566-79.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals.
Authors: Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A
Cancer Res, 2015;75(13):2653-62.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi.
Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W
Stem Cells, 2015;33(3):848-58.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop.
Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K
J Invest Dermatol, 2014;134(5):1295-304.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
G-CSF drives a posttraumatic immune program that protects the host from infection.
Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F
J Immunol, 2014;192(5):2405-17.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Types: Plasma
Applications: Luminex Development -
Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism.
Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI
PLoS ONE, 2011;6(11):e27690.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism.
Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G
PLoS ONE, 2011;6(5):e19957.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice.
Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH
J. Clin. Invest., 2011;121(11):4446-61.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Cannabinoid receptor activation leads to massive mobilization of myeloid-derived suppressor cells with potent immunosuppressive properties.
Authors: Hegde VL, Nagarkatti M, Nagarkatti PS
Eur. J. Immunol., 2010;40(12):3358-71.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models.
Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N
Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow.
Authors: Brunner S, Zaruba MM, Huber B, David R, Vallaster M, Assmann G, Mueller-Hoecker J, Franz WM
Exp. Hematol., 2008;36(9):1157-66.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse.
Authors: Furze RC, Rankin SM
FASEB J., 2008;22(9):3111-9.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Bv8 regulates myeloid-cell-dependent tumour angiogenesis.
Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N
Nature, 2007;450(7171):825-31.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation.
Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM
Blood, 2007;111(1):42-9.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization -
Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense.
Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK
J. Exp. Med., 2001;194(4):519-27.
Species: Mouse
Sample Types: BALF
Applications: ELISA Development -
Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure.
Authors: Kulkarni, Shilpa, Singh, Pankaj K, Ghosh, Sanchita, Posarac, Ana, Singh, Vijay K
Cytokine, 213;62(2):278-85.
Species: Mouse
Sample Types: In Vivo
Applications: Neutralization
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Reviews for Mouse G-CSF Antibody
Average Rating: 4 (Based on 3 Reviews)
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This material was use successfully as a capture antibody in our Mouse ELISA. The results were very sensitive.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***