Mouse G-CSF Antibody

Name: Mouse G-CSF Antibody
Brand: R&D Systems
Rating: 5 (1 reviews)

(27 citations)

(1 Review)

Datasheet / CoA / SDS

Neutralization

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Product Details

Citations (27)

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Species Reactivity

Mouse
Specificity

Detects mouse G-CSF in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human (rh) G‑CSF, rhCNTF, rmIL-6, rmLIF, or rmOSM.
Source

Monoclonal Rat IgG₁ Clone # 67604
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

E. coli-derived recombinant mouse G-CSF
Val31-Ala208
Accession # P09920
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse G-CSF (Catalog # 414-CS)

Mouse G-CSF Sandwich Immunoassay

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Mouse G‑CSF Antibody (Catalog # MAB414 )

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Mouse G‑CSF Biotinylated Antibody (Catalog # BAF414 )

ELISA Standard

Recombinant Mouse G-CSF Protein (Catalog # 414-CS )

Neutralization

Measured by its ability to neutralize G‑CSF-induced proliferation in the NFS‑60 mouse myeloid cells line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND₅₀) is typically 0.01-0.03 µg/mL in the presence of 0.125 ng/mL Recombinant Mouse G‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization

click image to view larger

Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF Antibody.

Recombinant Mouse G‑CSF (Catalog # 414-CS) stimulates proliferation in the NFS‑60 mouse myeloid cells line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G‑CSF (0.125 ng/mL) is neutralized (green line) by increasing concentrations of Mouse G‑CSF Monoclonal Antibody (Catalog # MAB414). The ND₅₀ is typically 0.01-0.03 µg/mL.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSF

G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic granulocyte lineage. It is produced mainly by monocytes and macrophages upon activation by endotoxin, TNF-alpha and IFN-gamma. Other cell types including fibroblasts, endothelial cells, astrocytes and bone marrow stromal cells can also secrete G-CSF after LPS, IL-1, or TNF-alpha activation. In addition, various carcinoma cell lines and myeloblastic leukemia cells can express G-CSF constitutively.

The murine G-CSF cDNA encodes a 208 amino acid (aa) residue precursor protein containing a 30 aa residue signal peptide that is proteolytically cleaved to generate the 178 aa residue mature protein. Human G-CSF is 73% identical at the amino acid level to murine G-CSF and the two proteins show species cross-reactivity.

In vitro, G-CSF stimulates growth, differentiation and functions of cells from the neutrophil lineage. It also has blast cell growth factor activity and can synergize with IL-3 to shorten the G_o period of early hematopoietic progenitors. Consistent with its in vitro functions, G-CSF has been found to play important roles in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Recombinant human G-CSF has been approved for the amelioration of chemotherapy induced neutropenia as well as for severe chronic neutropenia following marrow transplant.

Long Name:

Granulocyte Colony Stimulating Factor

Entrez Gene IDs:

1440 (Human); 12985 (Mouse)

Alternate Names:

C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3 (granulocyte); CSF3; CSF3OS; Filgrastim; GCSF; G-CSF; GCSFlenograstim; granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
Filter your results:

Species	Applications	Sample Type
Breast and pancreatic cancer interrupt IRF8-dependent dendritic cell development to overcome immune surveillance Authors: MA Meyer, JM Baer, BL Knolhoff, TM Nywening, RZ Panni, X Su, KN Weilbaeche, WG Hawkins, C Ma, RC Fields, DC Linehan, GA Challen, R Faccio, RL Aft, DG DeNardo Nat Commun, 2018;9(1):1250. Species: Mouse Sample Type: In Vivo Application: Neut Granulocyte colony-stimulating factor blockade enables dexamethasone to inhibit lipopolysaccharide-induced murine lung neutrophils Authors: J Banuelos, Y Cao, SC Shin, BS Bochner, P Avila, S Li, X Jiang, MW Lingen, RP Schleimer, NZ Lu PLoS ONE, 2017;12(5):e0177884. Species: Mouse Sample Type: In Vivo Application: Neut Reactive Oxygen Species-Producing Myeloid Cells Act as a Bone Marrow Niche for Sterile Inflammation-Induced Reactive Granulopoiesis Authors: H Zhu, HJ Kwak, P Liu, B Bajrami, Y Xu, SY Park, C Nombela-Ar, S Mondal, H Kambara, H Yu, L Chai, LE Silberstei, T Cheng, HR Luo J. Immunol, 2017;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo Depletion of CD11c(+) cells in the CD11c.DTR model drives expansion of unique CD64(+) Ly6C(+) monocytes that are poised to release TNF-alpha. Authors: Sivakumaran S, Henderson S, Ward S, Santos E Sousa P, Manzo T, Zhang L, Conlan T, Means T, D'Aveni M, Hermine O, Rubio M, Chakraverty R, Bennett C Eur J Immunol, 2016;46(1):192-203. Species: Mouse Sample Type: In Vivo Application: Neut Role of G-CSF in monophosphoryl lipid A-mediated augmentation of neutrophil functions after burn injury. Authors: Bohannon J, Luan L, Hernandez A, Afzal A, Guo Y, Patil N, Fensterheim B, Sherwood E J Leukoc Biol, 2016;99(4):629-40. Species: Mouse Sample Type: In Vivo Application: In Vivo Tumor-secreted products repress B-cell lymphopoiesis in a murine model of breast cancer Eur J Immunol, 2016;0(0):. Species: Mouse Sample Type: Whole Cells Application: Neut An NK Cell Perforin Response Elicited via IL-18 Controls Mucosal Inflammation Kinetics during Salmonella Gut Infection Authors: Anna A Müller PLoS Pathog, 2016;12(6):e1005723. Species: Mouse Sample Type: In Vivo Application: In vivo The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity Cell, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: In vivo Neutrophil Extracellular Traps Accumulate in Peripheral Blood Vessels and Compromise Organ Function in Tumor-Bearing Animals. Authors: Cedervall J, Zhang Y, Huang H, Zhang L, Femel J, Dimberg A, Olsson A Cancer Res, 2015;75(13):2653-62. Species: Mouse Sample Type: In Vivo Application: Neut IL-17-producing gammadelta T cells and neutrophils conspire to promote breast cancer metastasis. Authors: Coffelt S, Kersten K, Doornebal C, Weiden J, Vrijland K, Hau C, Verstegen N, Ciampricotti M, Hawinkels L, Jonkers J, de Visser K Nature, 2015;522(7556):345-8. Species: Mouse Sample Type: In Vivo Application: Neut Coordinate expansion of murine hematopoietic and mesenchymal stem cell compartments by SHIPi. Authors: Brooks R, Iyer S, Akada H, Neelam S, Russo C, Chisholm J, Kerr W Stem Cells, 2015;33(3):848-58. Species: Mouse Sample Type: In Vivo Application: Neut IL-17-mediated antifungal defense in the oral mucosa is independent of neutrophils. Authors: Trautwein-Weidner K, Gladiator A, Nur S, Diethelm P, LeibundGut-Landmann S Mucosal Immunol, 2015;8(2):221-31. Species: Mouse Sample Type: In Vivo G-CSF Promotes Neuroblastoma Tumorigenicity and Metastasis via STAT3-Dependent Cancer Stem Cell Activation. Authors: Agarwal S, Lakoma A, Chen Z, Hicks J, Metelitsa L, Kim E, Shohet J Cancer Res, 2015;75(12):2566-79. Species: Mouse Sample Type: Whole Cells Application: Flow Periostin controls keratinocyte proliferation and differentiation by interacting with the paracrine IL-1alpha/IL-6 loop. Authors: Taniguchi K, Arima K, Masuoka M, Ohta S, Shiraishi H, Ontsuka K, Suzuki S, Inamitsu M, Yamamoto K, Simmons O, Toda S, Conway S, Hamasaki Y, Izuhara K J Invest Dermatol, 2014;134(5):1295-304. Species: Mouse Sample Type: Whole Cells Application: Neut G-CSF drives a posttraumatic immune program that protects the host from infection. Authors: Gardner J, Noel J, Nikolaidis N, Karns R, Aronow B, Ogle C, McCormack F J Immunol, 2014;192(5):2405-17. Species: Mouse Sample Type: In Vivo Application: In vivo Response patterns of cytokines/chemokines in two murine strains after irradiation. Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Cytokine, 2012;58(2):169-77. Species: Mouse Sample Type: Plasma Application: Luminex Assay Development Activation of MDL-1 (CLEC5A) on immature myeloid cells triggers lethal shock in mice. Authors: Cheung R, Shen F, Phillips JH, McGeachy MJ, Cua DJ, Heyworth PG, Pierce RH J. Clin. Invest., 2011;121(11):4446-61. Species: Mouse Sample Type: In Vivo Application: In vivo Tumor-derived G-CSF facilitates neoplastic growth through a granulocytic myeloid-derived suppressor cell-dependent mechanism. Authors: Waight JD, Hu Q, Miller A, Liu S, Abrams SI PLoS ONE, 2011;6(11):e27690. Species: Mouse Sample Type: In Vivo Application: In vivo Inflammation Triggers Emergency Granulopoiesis through a Density-Dependent Feedback Mechanism. Authors: Cain DW, Snowden PB, Sempowski GD, Kelsoe G PLoS ONE, 2011;6(5):e19957. Species: Mouse Sample Type: In Vivo Application: In vivo Cannabinoid receptor activation leads to massive mobilization of myeloid-derived suppressor cells with potent immunosuppressive properties. Authors: Hegde VL, Nagarkatti M, Nagarkatti PS Eur. J. Immunol., 2010;40(12):3358-71. Species: Mouse Sample Type: In Vivo Application: In vivo G-CSF-initiated myeloid cell mobilization and angiogenesis mediate tumor refractoriness to anti-VEGF therapy in mouse models. Authors: Shojaei F, Wu X, Qu X, Kowanetz M, Yu L, Tan M, Meng YG, Ferrara N Proc. Natl. Acad. Sci. U.S.A., 2009;106(16):6742-7. Species: Mouse Sample Type: In Vivo Application: In vivo Parathyroid hormone effectively induces mobilization of progenitor cells without depletion of bone marrow. Authors: Brunner S, Zaruba MM, Huber B, David R, Vallaster M, Assmann G, Mueller-Hoecker J, Franz WM Exp. Hematol., 2008;36(9):1157-66. Species: Mouse Sample Type: In Vivo Application: In vivo The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse. Authors: Furze RC, Rankin SM FASEB J., 2008;22(9):3111-9. Species: Mouse Sample Type: In Vivo Application: In vivo Bv8 regulates myeloid-cell-dependent tumour angiogenesis. Authors: Shojaei F, Wu X, Zhong C, Yu L, Liang XH, Yao J, Blanchard D, Bais C, Peale FV, van Bruggen N, Ho C, Ross J, Tan M, Carano RA, Meng YG, Ferrara N Nature, 2007;450(7171):825-31. Species: Mouse Sample Type: In Vivo Application: In vivo The coordinated action of G-CSF and ELR + CXC chemokines in neutrophil mobilization during acute inflammation. Authors: Wengner AM, Pitchford SC, Furze RC, Rankin SM Blood, 2007;111(1):42-9. Species: Mouse Sample Type: In Vivo Application: In vivo Requirement of interleukin 17 receptor signaling for lung CXC chemokine and granulocyte colony-stimulating factor expression, neutrophil recruitment, and host defense. Authors: Ye P, Rodriguez FH, Kanaly S, Stocking KL, Schurr J, Schwarzenberger P, Oliver P, Huang W, Zhang P, Zhang J, Shellito JE, Bagby GJ, Nelson S, Charrier K, Peschon JJ, Kolls JK J. Exp. Med., 2001;194(4):519-27. Species: Mouse Sample Type: BALF Application: ELISA Development Granulocyte colony-stimulating factor antibody abrogates radioprotective efficacy of gamma-tocotrienol, a promising radiation countermeasure. Authors: Kulkarni, Shilpa, Singh, Pankaj K, Ghosh, Sanchita, Posarac, Ana, Singh, Vijay K Cytokine, 213;62(2):278-85. Species: Mouse Sample Type: In Vivo Application: Neut Expand to show all Citations

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Isotype Controls

Description Application Cat# Citations Images

Rat IgG1 Isotype Control

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Mouse G-CSF Antibody

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Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Rat IgG1 Isotype Control

Normal Rat IgG Control

Normal Rat IgG Control (Azide Free)

Rat IgG1 Isotype Control

Reconstitution Buffer 1 (PBS)

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Goat Anti-Rat IgG Biotinylated Antibody

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Rat IgG VisUCyte HRP Polymer Antibody

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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