Mouse Gas6 Antibody
Mouse Gas6 Antibody Summary
Asp115-Pro673
Accession # CAA42507
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Applications
Mouse Gas6 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Gas6 in Mouse Intestine. Gas6 was detected in perfusion fixed paraffin-embedded sections of mouse intestine using Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gas6
Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.
Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.
- Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
- Goruppi, S. et al. (1996) Oncogene 12:471.
- Crosier, K and P. Crosier (1997) Pathology 29:131.
- Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
- Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.
Product Datasheets
Citations for Mouse Gas6 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Gas6 induces inflammation and reduces plaque burden but worsens behavior in a sex-dependent manner in the APP/PS1 model of Alzheimer's disease
Authors: LD Owlett, B Karaahmet, L Le, EK Belcher, D Dionisio-S, JA Olschowka, MR Elliott, MK O'Banion
Journal of Neuroinflammation, 2022;19(1):38.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Microglia use TAM receptors to detect and engulf amyloid &beta plaques
Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
Nature Immunology, 2021;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gamma-glutamyl carboxylated Gas6 mediates the beneficial effect of vitamin K on lowering hyperlipidemia via regulating the AMPK/SREBP1/PPAR? signaling cascade of lipid metabolism
Authors: J Bordoloi, D Ozah, T Bora, J Kalita, P Manna
J. Nutr. Biochem., 2019;70(0):174-184.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Immunoprecipitation -
GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa
Authors: M Nassar, Y Tabib, T Capucha, G Mizraji, T Nir, M Pevsner-Fi, G Zilberman-, O Heyman, G Nussbaum, H Bercovier, A Wilensky, E Elinav, T Burstyn-Co, AH Hovav
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
TAM receptors regulate multiple features of microglial physiology
Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
Nature, 2016;532(7598):240-4.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
Authors: Farkas M, Lew D, Sousa M, Bujakowska K, Chatagnon J, Bhattacharya S, Pierce E, Nandrot E
Am J Pathol, 2014;184(10):2641-52.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Diversification of TAM receptor tyrosine kinase function.
Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
Nat Immunol, 2014;15(10):920-8.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis.
Neuron, 2012;76(6):1123-32.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
Authors: Galvan MD, Foreman DB, Zeng E
J. Immunol., 2012;188(8):3716-23.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Role of Gas6 in erythropoiesis and anemia in mice.
Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
J. Clin. Invest., 2008;118(2):583-96.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
Blood, 2007;109(3):1026-33.
Species: Mouse
Sample Types: Serum
Applications: Immunoprecipitation
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