Mouse Gas6 Antibody

Catalog # Availability Size / Price Qty
AF986
AF986-SP
Gas6 in Mouse Intestine.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)

Mouse Gas6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Gas6 in ELISAs and Western blots. In sandwich immunoassays, less than 10% cross-reactivity with recombinant human Gas6 is observed and less than 1% cross-reactivity with recombinant mouse Gas1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Gas6 (R&D Systems, Catalog # 986-GS)
Asp115-Pro673
Accession # CAA42507
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
1-15 µg/mL
See below

Mouse Gas6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Gas6 Biotinylated Antibody (Catalog # BAF986)

Standard: Recombinant Mouse Gas6 Protein (Catalog # 986-GS)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Gas6 antibody in Mouse Intestine by Immunohistochemistry (IHC-P). View Larger

Gas6 in Mouse Intestine. Gas6 was detected in perfusion fixed paraffin-embedded sections of mouse intestine using Goat Anti-Mouse Gas6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF986) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to apical plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Gas6

Growth arrest-specific gene 6 (Gas6) was initially characterized as a gene whose expression was up-regulated in serum starved NIH 3T3 fibroblasts and whose expression is down-regulated during growth induction. Mouse Gas6 is a 673 amino acid protein that shares 81% identity to its human homolog. Gas6 is a member of the vitamin K-dependent family of proteins that includes human protein S, a negative coregulator in the blood coagulation pathway. Gas6 and protein S share structural motifs that characterize this family: an extensively gamma -carboxylated amino terminus (Gla domain), four EGF-like repeats, and a carboxy terminus containing globular (G) domains with homology to steroid hormone-binding globulin. It is a ligand for the Axl (Ufo/Ark), Sky (Dtk/Tyro3/Rse/Brt/Tif), and Mer (Eyk) families of tyrosine kinase receptors. Gas6 binds to these receptors via tandem G domains at its C-terminus. Gas6 is ubiquitously expressed, but most abundantly in lung, intestine, bone marrow and endothelium.

Gas6 has been implicated in a variety of biological processes. It serves as a mitogen for fibroblasts, endothelial cells, neural cells, vascular smooth muscle cells and several tumor-derived cell lines. In addition, Gas6 prevents apoptosis, independent of its mitogenic activity. Gas6 is capable of inducing cell adhesion and chemotaxis in specific cell types. It also supports vitamin K-independent hematopoiesis when expressed by stromal cells. Gas6 signal transduction following Axl/Sky/Mer receptor activation has been reported to occur through such diverse signaling pathways as PI3K, MAP kinase, Src, Ras and beta -catenin.

References
  1. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  2. Goruppi, S. et al. (1996) Oncogene 12:471.
  3. Crosier, K and P. Crosier (1997) Pathology 29:131.
  4. Dormady, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97:12260.
  5. Goruppi, S. et al. (2001) Mol. Cell Biol. 21:902.
Long Name
Growth-arrest-specific Protein 6
Entrez Gene IDs
2621 (Human); 14456 (Mouse)
Alternate Names
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; DKFZp666G247; FLJ34709; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6

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Citations for Mouse Gas6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Gas6 ameliorates intestinal mucosal immunosenescence to prevent the translocation of a gut pathobiont, Klebsiella pneumoniae, to the liver
    Authors: Tsugawa, H;Ohki, T;Tsubaki, S;Tanaka, R;Matsuzaki, J;Suzuki, H;Hozumi, K;
    PLoS pathogens
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Gas6 induces inflammation and reduces plaque burden but worsens behavior in a sex-dependent manner in the APP/PS1 model of Alzheimer's disease
    Authors: LD Owlett, B Karaahmet, L Le, EK Belcher, D Dionisio-S, JA Olschowka, MR Elliott, MK O'Banion
    Journal of Neuroinflammation, 2022-02-07;19(1):38.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  3. Microglia use TAM receptors to detect and engulf amyloid &beta plaques
    Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
    Nature Immunology, 2021-04-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Gamma-glutamyl carboxylated Gas6 mediates the beneficial effect of vitamin K on lowering hyperlipidemia via regulating the AMPK/SREBP1/PPAR? signaling cascade of lipid metabolism
    Authors: J Bordoloi, D Ozah, T Bora, J Kalita, P Manna
    J. Nutr. Biochem., 2019-05-25;70(0):174-184.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Immunoprecipitation
  5. GAS6 is a key homeostatic immunological regulator of host-commensal interactions in the oral mucosa
    Authors: M Nassar, Y Tabib, T Capucha, G Mizraji, T Nir, M Pevsner-Fi, G Zilberman-, O Heyman, G Nussbaum, H Bercovier, A Wilensky, E Elinav, T Burstyn-Co, AH Hovav
    Proc. Natl. Acad. Sci. U.S.A, 2017-01-03;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. TAM receptors regulate multiple features of microglial physiology
    Authors: L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke
    Nature, 2016-04-06;532(7598):240-4.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer's disease.
    Authors: Savage J, Jay T, Goduni E, Quigley C, Mariani M, Malm T, Ransohoff R, Lamb B, Landreth G
    J Neurosci, 2015-04-22;35(16):6532-43.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  8. Diversification of TAM receptor tyrosine kinase function.
    Authors: Zagorska A, Traves P, Lew E, Dransfield I, Lemke G
    Nat Immunol, 2014-09-07;15(10):920-8.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  9. Mutations in pre-mRNA processing factors 3, 8, and 31 cause dysfunction of the retinal pigment epithelium.
    Authors: Farkas M, Lew D, Sousa M, Bujakowska K, Chatagnon J, Bhattacharya S, Pierce E, Nandrot E
    Am J Pathol, 2014-08-08;184(10):2641-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis.
    Neuron, 2012-12-20;76(6):1123-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Complement component C1q regulates macrophage expression of Mer tyrosine kinase to promote clearance of apoptotic cells.
    Authors: Galvan MD, Foreman DB, Zeng E
    J. Immunol., 2012-03-14;188(8):3716-23.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Role of Gas6 in erythropoiesis and anemia in mice.
    Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
    J. Clin. Invest., 2008-02-01;118(2):583-96.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. A soluble form of the Mer receptor tyrosine kinase inhibits macrophage clearance of apoptotic cells and platelet aggregation.
    Authors: Sather S, Kenyon KD, Lefkowitz JB, Liang X, Varnum BC, Henson PM, Graham DK
    Blood, 2006-10-17;109(3):1026-33.
    Species: Mouse
    Sample Types: Serum
    Applications: Immunoprecipitation, Western Blot

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Mouse Gas6 Antibody
By Anonymous on 01/04/2019
Application: WB Sample Tested: Liver tissue Species: Mouse