Mouse GDF-5/BMP-14 Antibody

Catalog # Availability Size / Price Qty
AF853
AF853-SP
GDF‑5 in Mouse Embryo.
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Product Details
Citations (4)
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Mouse GDF-5/BMP-14 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF‑9, recombinant human GDF-11, and rhGDF-15 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF-5
Ala376-Arg495
Accession # P43027
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
NIH‑3T3 mouse embryonic fibroblast cell line
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry GDF-5 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

GDF‑5 in Mouse Embryo. GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Western Blot View Larger

Detection of Mouse GDF‑5/BMP‑14 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse GDF‑5/BMP‑14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑5/BMP‑14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region, and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6, and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H. Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.
Long Name
Growth Differentiation Factor 5
Entrez Gene IDs
8200 (Human); 14563 (Mouse)
Alternate Names
BMP14; BMP-14; Cartilage-derived morphogenetic protein 1; cartilage-derived morphogenetic protein-1; CDMP-1; CDMP1LAP4; GDF5; GDF-5; growth differentiation factor 5; growth/differentiation factor 5; OS5; radotermin; SYNS2

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Citations for Mouse GDF-5/BMP-14 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. GDF5 Mediated Enhancement of Chondrocyte Phenotype is Inhibited by Heparin; Implication for the use of Heparin in the Clinic and in Tissue Engineering Applications
    Authors: Bethanie Imogen Ayerst
    Tissue Eng Part A, 2017-01-06;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  2. Joint TGF-beta type II receptor-expressing cells: ontogeny and characterization as joint progenitors.
    Authors: Li T, Longobardi L, Myers T, Temple J, Chandler R, Ozkan H, Contaldo C, Spagnoli A
    Stem Cells Dev, 2013-02-15;22(9):1342-59.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Growth differentiation factor 5 regulates cardiac repair after myocardial infarction.
    Authors: Zaidi SH, Huang Q, Momen A, Riazi A, Husain M
    J. Am. Coll. Cardiol., 2010-01-12;55(2):135-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Freeze-dried tendon allografts as tissue-engineering scaffolds for Gdf5 gene delivery.
    Authors: Basile P, Dadali T, Jacobson J, Hasslund S, Ulrich-Vinther M, Soballe K, Nishio Y, Drissi MH, Langstein HN, Mitten DJ, O'Keefe RJ, Schwarz EM, Awad HA
    Mol. Ther., 2008-01-08;16(3):466-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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