Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Amphibian - Ambystoma mexicanum (Axolotl), Equine

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GDF-5/BMP-14 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GDF-5
Ala376-Arg495
Accession # P43027

Specificity

Detects mouse GDF-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse (rm) GDF-6 is observed, and less than 5% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF‑9, recombinant human GDF-11, and rhGDF-15 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse GDF‑5/BMP‑14 Antibody

GDF-5 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

GDF‑5 in Mouse Embryo.

GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse GDF‑5/BMP‑14 by Western Blot.

Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse GDF‑5/BMP‑14 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑5/BMP‑14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Applications for Mouse GDF‑5/BMP‑14 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embyro (12 d.p.c.)

Western Blot

1 µg/mL
Sample: NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-5/BMP-14

Growth Differentiation Factor 5 (GDF-5), also known as cartilage-derived morphogenetic protein 1 (CDMP-1), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. GDF-5 is synthesized as a large precursor protein that consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aa pro region, and a 120 aa C-terminal mature peptide. Mature GDF-5 is a homodimeric protein which contains the characteristic seven conserved cysteine residues. GDF-5, GDF-6, and GDF-7, which share 80-86% identity, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-5 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-5 are 98% identical. It has been reported that GDF-5 has multiple functions including regulation of myogenesis, regulation of chondrogenesis, bone morphogenesis, and neuron differentiation and survival. GDF-5 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins (Smad 1, 5, and 8).

References

  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
  5. Settle, S.H. Jr. et al. (2003) Dev. Biol. 254:116.
  6. Inada, M. et al. (1996) BBRC 222:317.

Long Name

Growth Differentiation Factor 5

Alternate Names

BMP-14, CDMP-1, GDF5

Entrez Gene IDs

8200 (Human); 14563 (Mouse)

Gene Symbol

GDF5

UniProt

P43027

Additional GDF-5/BMP-14 Products

Product Documents for Mouse GDF‑5/BMP‑14 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse GDF‑5/BMP‑14 Antibody

For research use only

Citations for Mouse GDF‑5/BMP‑14 Antibody

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Protocols

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