Mouse GDF-8/Myostatin Propeptide Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1539

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Mouse GDF-8/Myostatin Propeptide Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF‑8/Myostatin Propeptide in Western blots. In Western blots, less than 5% cross-reactivity with mature recombinant mouse GDF-1, -3, -5, -6, -7, -8, -9 and recombinant human GDF-11 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GDF‑8/Myostatin Propeptide
Asn25-Ser265
Accession # Q540E2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GDF‑8/Myostatin Propeptide (Catalog # 1539-PG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-8/Myostatin

Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-beta superfamily protein that is expressed specifically in developing and adult skeletal muscle. It controls myoblast proliferation and is a potent negative regulator of skeletal muscle mass (1‑3). Mouse GDF-8 is synthesized as a 376 amino acid (aa) preproprotein that consists of a 24 aa signal peptide, a 243 aa propeptide, and a 109 aa mature protein (2). Within the propeptide, mouse GDF-8 shares 96% and 99% aa sequence identity with human and rat GDF-8, respectively. GDF-8 is secreted as a preproprotein that is cleaved by BMP-1 family proteases to separate the 35‑40 kDa propeptide from the 12 kDa bioactive mature protein (4‑6). This results in a latent complex containing a disulfide-linked dimer of the mature protein and two noncovalently-associated molecules of the propeptide (2, 6). The GDF-8 propeptide functions as an inhibitor of mature GDF-8, and GDF-8 activity can also be inhibited through association with Follistatin, FLRG, Decorin, or GASP-1 (6‑11). The uncleaved GDF-8 proprotein binds Latent TGF-beta bp3 which can sequester it in the extracellular matrix and prevent the proteolytic cleavage of the propeptide (12). GDF-8 binds to the type II Activin receptor Activin RIIB which then associates with the type I receptors Activin RIB/ALK-4 or TGF-beta RI/ALK-5 to induce signaling (13). GDF-8 additionally inhibits adipogenic differentiation of mesenchymal stem cells and preadipocytes (14). Genetic deletion of GDF-8 or in vivo administration of the GDF-8 propeptide induces muscle hypertrophy as well as enhanced glucose utilization and insulin sensitivity and a reduction in overall fat mass (15, 16).

References
  1. McPherron, A.C. (2010) Immunol. Endocr. Metab. Agents Med. Chem. 10:217.
  2. McPherron, A.C. et al. (1997) Nature 387:83.
  3. Zimmers, T.A. et al. (2002) Science 296:1486.
  4. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. 100:15842.
  5. McFarlane, C. et al. (2005) Dev. Biol. 283:58.
  6. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. 98:9306.
  7. Thies, R.S. et al. (2001) Growth Factors 18:251.
  8. Amthor, H. et al. (2004) Dev. Biol. 270:19.
  9. Hill, J.J. et al. (2002) J. Biol. Chem. 277:40735.
  10. Miura, T. et al. (2006) Biochem. Biophys. Res. Commun. 340:675.
  11. Hill, J.J. et al. (2003) Molecular Endocrinology 17:1144.
  12. Anderson, S.B. et al. (2008) J. Biol. Chem. 283:7027.
  13. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230.
  14. Guo, W. et al. (2008) J. Biol. Chem. 283:9136.
  15. Matsakas, A. et al. (2009) Neuromuscul. Disord. 19:489.
  16. Guo, T. et al. (2009) PloS ONE 4:e4937.
Long Name
Growth Differentiation Factor 8
Entrez Gene IDs
2660 (Human); 17700 (Mouse)
Alternate Names
GDF8; GDF-8; GDF8growth differentiation factor 8; growth/differentiation factor 8; MSLHP; MSTN; Myostatin

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