Mouse GFR alpha-4/GDNF R alpha-4 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1677
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Mouse GFR alpha-4/GDNF R alpha-4 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GFR alpha ‑4/GDNF R alpha ‑4 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) GFR alpha -4 is observed and less than 1% cross‑reactivity with rhGFR alpha -3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑4/GDNF R alpha ‑4
aa 35-242
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GFR alpha ‑4/GDNF R alpha ‑4
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse brain (medulla)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-4/GDNF R alpha-4

GFR alpha -4 is a GPI-linked cell surface protein that associates with the c-Ret transmembrane tyrosine kinase to form a functional receptor complex for the GDNF family ligand, persephin.

Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 4
Entrez Gene IDs
64096 (Human); 14588 (Mouse)
Alternate Names
GDNF family receptor alpha 4; GDNF family receptor alpha-4; GDNF R alpha-4; GDNF receptor alpha-4; GDNFR-alpha-4; GFR alpha4; GFR alpha-4; GFR receptor alpha 4; GFRA4; GFRa-4; GFR-alpha-4; Persephin receptor

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