Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GFR alpha-4/GDNF R alpha-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse GFR alpha ‑4/GDNF R alpha ‑4
Asn24-Thr237
Accession # Q99JT2

Specificity

Detects mouse GFR alpha ‑4/GDNF R alpha ‑4 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human GFR alpha -4 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse GFR alpha ‑4/GDNF R alpha ‑4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (medulla)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-4/GDNF R alpha-4

GFR alpha -4 is a GPI-linked cell surface protein that associates with the c-Ret transmembrane tyrosine kinase to form a functional receptor complex for the GDNF family ligand, persephin.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 4

Alternate Names

GDNF R alpha-4, GFR alpha4, GFRa-4, GFRA4

Entrez Gene IDs

64096 (Human); 14588 (Mouse)

Gene Symbol

GFRA4

UniProt

Q99JT2

Additional GFR alpha-4/GDNF R alpha-4 Products

Product Documents for Mouse GFR alpha ‑4/GDNF R alpha ‑4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse GFR alpha ‑4/GDNF R alpha ‑4 Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Mouse GFR alpha ‑4/GDNF R alpha ‑4 Antibody

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Protocols

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FAQs

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