Detects mouse GITR Ligand/TNFSF18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) APRIL, rmBAFF, rmEDA, rmFas Ligand, rmLIGHT, rmOX40 Ligand, rmTNF-alpha, rmTRAIL, rmTRANCE, rmTWEAK, rm4-1BB Ligand, recombinant human (rh) EDA-A2, rhLTa1/b2, rhLTa2/b1, rhVEGI, or rhGITR Ligand is observed. In Western blots, no cross-reactivity with rm4‑1BB, rmEDA, rmLIGHT, rmTRAIL, rhGITR Ligand, rhTNF-alpha, recombinant canine TNF‑ alpha, recombinant guinea pig TNF-alpha, or recombinant porcine TNF-alpha is observed.
Monoclonal Rat IgG1 Clone # 721926
Protein A or G purified from hybridoma culture supernatant
Detection of Mouse GITR Ligand/TNFSF18 by Western Blot.
Western blot shows lysates of A20 mouse B cell lymphoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse GITR Ligand/TNFSF18 Monoclonal Antibody (Catalog # MAB21772) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for GITR Ligand/TNFSF18 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GITR Ligand/TNFSF18
Glucocorticoid-induced TNF receptor superfamily-related protein ligand (GITRL) is a member of the TNF superfamily (TNFSF) and has been designated TNFSF18. Mouse GITRL cDNA encodes a 173 amino acid (aa) type II membrane protein with a C-terminal extracellular domain of 131 aa, an N-terminal cytoplasmic domain of 23 aa and a transmembrane domain of 19 aa. It shares approximately 60% aa sequence identity with human GITRL (2). Mouse GITRL is expressed at high levels in macrophages, dendritic cells and B cells. The expression is transiently upregulated by LPS stimulation. GITRL binds to the type I transmembrane protein GITR/TNFRSF18, which is a member of the TNF receptor superfamily that is predominantly expressed on CD25+ regulatory CD4+ T cells (Treg). GITR is also expressed on naïve CD4+ CD25- T cells, where its expression is upregulated after antigen-driven activation (1, 2). Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2. GITRL provides costimulatory signals for activated CD4+ CD25- T cells to enhance cell proliferation and augment cytokine production. On CD4+ CD25+ Treg cells, GITRL also provides costimulatory signals to induce proliferation, setting Treg cells in an active/hyperproliferactive state that reverses the suppressive function of Treg cells. GITRL-GITR ligation provides important costimulatory signals that play important roles in modulating diverse T cell functions (1-4).
Tone, M. et al. (2003) Proc. Natl. Acad. Sci. USA 100:15059.
Ji, H. et al. (2004) J. Immunology 172:5823.
Kanamaru, F. et al. (2004) J. Immunology 172:7306.
Ronchetti, S. et al. (2004) Eur. J. Immunology 34:613.
Glucocorticoid Induced TNF Receptor Family Related Gene Ligand
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