Mouse GLI-2 Antibody
Mouse GLI-2 Antibody Summary
Met1-Glu416
Accession # NP_001074594
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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GLI‑2 in Mouse Embryo. GLI-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse GLI-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3635) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to developing muscle. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-2
Mouse GLI-2 is a 185 kDa biopotential transcription regulator of the hedgehog signaling pathway. It contains 5 conserved tandem C2H2 zinc finger domains that are flanked by a repression domain at the N-terminal region and an activation domain in the C-terminal region. At least two additional isoforms that have amino acid substitutions/truncations in the region corresponding to amino acid residues 192‑350 of isoform 1 have been described. Transcriptional activity of GLI-2 is regulated by proteolytic processing and degradation. Within the region used as immunogen, mouse GLI-2 shares 91% and 97% amino acid sequence homology with that of human and rat GLI-2, respectively.
Product Datasheets
Citations for Mouse GLI-2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Zic5 stabilizes Gli3 via a non-transcriptional mechanism during retinal development
Authors: J Sun, J Yoon, M Lee, HK Lee, YS Hwang, IO Daar
Cell Reports, 2022-02-01;38(5):110312.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot -
Activation of Cilia-Independent Hedgehog/GLI1 Signaling as a Novel Concept for Neuroblastoma Therapy
Authors: A Koeniger, A Brichkina, I Nee, L Dempwolff, A Hupfer, I Galperin, F Finkernage, A Nist, T Stiewe, T Adhikary, W Diederich, M Lauth
Cancers, 2021-04-15;13(8):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
A Shh/Gli-driven three-node timer motif controls temporal identity and fate of neural stem cells
Authors: JM Dias, Z Alekseenko, A Jeggari, M Boareto, J Vollmer, M Kozhevniko, H Wang, MP Matise, A Alexeyenko, D Iber, J Ericson
Science Advances, 2020-09-16;6(38):.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Immunoprecipitation, Western Blot -
A Smo/Gli Multitarget Hedgehog Pathway Inhibitor Impairs Tumor Growth
Authors: L Lospinoso, D Quaglio, I Basili, F Ghirga, F Bufalieri, M Caimano, S Balducci, M Moretti, I Romeo, E Loricchio, M Maroder, B Botta, M Mori, P Infante, L Di Marcotu
Cancers (Basel), 2019-10-09;11(10):.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot -
NRF2-dependent gene expression promotes ciliogenesis and Hedgehog signaling
Authors: A Martin-Hur, R Martin-Mor, N Robledinos, R Blanco, I Palacios-B, I Lastres-Be, A Cuadrado, FR Garcia-Gon
Sci Rep, 2019-09-25;9(1):13896.
Species: Mouse
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Western Blot -
Fine-Tuning of Shh/Gli Signaling Gradient by Non-proteolytic Ubiquitination during Neural Patterning
Authors: P Ma, NN Song, Y Li, Q Zhang, L Zhang, L Zhang, Q Kong, L Ma, X Yang, B Ren, C Li, X Zhao, Y Li, Y Xu, X Gao, YQ Ding, B Mao
Cell Rep, 2019-07-09;28(2):541-553.e4.
Species: Human, Mouse
Sample Types: Cell Fractions, Cell Lysates
Applications: Western Blot -
Itch/?-arrestin2-dependent non-proteolytic ubiquitylation of SuFu controls Hedgehog signalling and medulloblastoma tumorigenesis
Authors: P Infante, R Faedda, F Bernardi, F Bufalieri, L Lospinoso, R Alfonsi, D Mazzà, M Siler, S Coni, A Po, M Petroni, E Ferretti, M Mori, E De Smaele, G Canettieri, C Capalbo, M Maroder, I Screpanti, M Kool, SM Pfister, D Guardavacc, A Gulino, L Di Marcotu
Nat Commun, 2018-03-07;9(1):976.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
TSC1 and TSC2 regulate cilia length and canonical Hedgehog signaling via different mechanisms
Authors: T Rosengren, LJ Larsen, LB Pedersen, ST Christense, LB Møller
Cell. Mol. Life Sci., 2018-02-02;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
EMT programs promote basal mammary stem cell and tumor-initiating cell stemness by inducing primary ciliogenesis and Hedgehog signaling
Authors: VJ Guen, TE Chavarria, C Kröger, X Ye, RA Weinberg, JA Lees
Proc. Natl. Acad. Sci. U.S.A., 2017-11-20;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Suppressor of Fused Restraint of Hedgehog Activity Level is Critical for Osteogenic Proliferation and Differentiation during Calvarial Bone Development
Authors: J Li, Y Cui, J Xu, Q Wang, X Yang, Y Li, X Zhang, M Qiu, Z Zhang, Z Zhang
J. Biol. Chem., 2017-08-09;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Regulation of Gli ciliary localization and Hedgehog signaling by the PY-NLS/karyopherin-?2 nuclear import system
Authors: Y Han, Y Xiong, X Shi, J Wu, Y Zhao, J Jiang
PLoS Biol., 2017-08-04;15(8):e2002063.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Spop regulates Gli3 activity and Shh signaling in dorsoventral patterning of the mouse spinal cord
Authors: H Cai, A Liu
Dev. Biol., 2017-04-12;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Spop promotes skeletal development and homeostasis by positively regulating Ihh signaling
Proc. Natl. Acad. Sci. U.S.A, 2016-12-05;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent
PLoS ONE, 2016-08-31;11(8):e0162033.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Pharmacological GLI2 inhibition prevents myofibroblast cell-cycle progression and reduces kidney fibrosis.
Authors: Kramann R, Fleig S, Schneider R, Fabian S, DiRocco D, Maarouf O, Wongboonsin J, Ikeda Y, Heckl D, Chang S, Rennke H, Waikar S, Humphreys B
J Clin Invest, 2015-07-20;125(8):2935-51.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Inversion of Sonic hedgehog action on its canonical pathway by electrical activity.
Authors: Belgacem Y, Borodinsky L
Proc Natl Acad Sci U S A, 2015-03-17;112(13):4140-5.
Species: Xenopus
Sample Types: Embryo
Applications: IHC-P, Western Blot -
Ciliopathy proteins regulate paracrine signaling by modulating proteasomal degradation of mediators.
Authors: Liu Y, Tsai I, Morleo M, Oh E, Leitch C, Massa F, Lee B, Parker D, Finley D, Zaghloul N, Franco B, Katsanis N
J Clin Invest, 2014-04-01;124(5):2059-70.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The output of Hedgehog signaling is controlled by the dynamic association between Suppressor of Fused and the Gli proteins.
Authors: Humke EW, Dorn KV, Milenkovic L, Scott MP, Rohatgi R
Genes Dev., 2010-04-01;24(7):670-82.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation, Western Blot
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Reviews for Mouse GLI-2 Antibody
Average Rating: 3 (Based on 3 Reviews)
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This antibody recognizes many bands, and the major expected bands are not stronger (or maybe much weaker) than those non-specific bands.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***
