Mouse Glut2 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1440P-100UG

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Detection of Glut2 in  beta TC‑6 Mouse Cell Line by Flow Cytometry.
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Mouse Glut2 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Glut2. Recognizes mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (1).
Source
Monoclonal Rat IgG2B Clone # 205115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with mouse Glut2
Met1-Val523
Accession # P14246
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Glut2 antibody in  beta TC-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Glut2 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Mouse Glut2 PE-conjugated Monoclonal Antibody (Catalog # FAB1440P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Glut2

Glut2, also known as SLC2A2, belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane glycoprotein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells, and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1, 2).

References
  1. Poitout, V. et al. (1995) Diabetes 44:306.
  2. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
Long Name
Glucose Transporter Type 2
Entrez Gene IDs
6514 (Human); 20526 (Mouse)
Alternate Names
GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2

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