Mouse Glut2 Antibody Summary
Accession # P14246
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Glut2 in beta TC‑6 Mouse Cell Line. Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Monoclonal Antibody (Catalog # MAB1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Glut2 in beta TC-6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Mouse Glut2 Monoclonal Antibody (Catalog # MAB1440, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).
Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
Poitout, V. et al. (1995) Diabetes 44:306.
Citation for Mouse Glut2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Cholesterol Elevation Impairs Glucose-Stimulated Ca2+ Signaling in Mouse Pancreatic beta-Cells.
Authors: Lee AK, Yeung-Yam-Wah V, Tse FW, Tse A
Sample Types: Whole Tissue
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