Mouse Glut2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1440
Glut2 in  beta TC‑6 Mouse Cell Line.
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Product Details
Citations (1)
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Mouse Glut2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Glut2. Stains mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2).
Source
Monoclonal Rat IgG2B Clone # 205115
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with mouse Glut2
Met1-Val523
Accession # P14246
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
beta TC‑6 mouse beta cell insulinoma cell line
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Glut2 antibody in  beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Glut2 in beta TC‑6 Mouse Cell Line. Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Biotinylated Monoclonal Antibody (Catalog # BAM1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta -cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta -cells. Glut2 facilitates either glucose uptake or efflux from cells depending on the nutritional state (1).

References
  1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
  2. Poitout, V. et al. (1995) Diabetes 44:306.
Long Name
Glucose Transporter Type 2
Entrez Gene IDs
6514 (Human); 20526 (Mouse)
Alternate Names
GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2

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Citation for Mouse Glut2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
    Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
    J. Immunol., 2011-08-17;187(6):3096-103.
    Species: Human, Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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