Mouse Glut2 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Glut2. Stains mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2).
  • Source
    Monoclonal Rat IgG2B Clone # 205115
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO Chinese hamster ovary cell line transfected with mouse Glut2
    Met1-Val523
    Accession # P14246
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    beta TC‑6 mouse beta cell insulinoma cell line
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Glut2 in beta TC‑6 Mouse Cell Line. Glut2 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Biotinylated Monoclonal Antibody (Catalog # BAM1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta -cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta -cells. Glut2 facilitates either glucose uptake or efflux from cells depending on the nutritional state (1).

  • References:
    1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
    2. Poitout, V. et al. (1995) Diabetes 44:306.
  • Long Name:
    Glucose Transporter Type 2
  • Entrez Gene IDs:
    6514 (Human); 20526 (Mouse)
  • Alternate Names:
    GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Recognition and killing of human and murine pancreatic beta cells by the NK receptor NKp46.
    Authors: Gur C, Enk J, Kassem SA, Suissa Y, Magenheim J, Stolovich-Rain M, Nir T, Achdout H, Glaser B, Shapiro J, Naparstek Y, Porgador A, Dor Y, Mandelboim O
    J. Immunol., 2011;187(6):3096-103.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Biotinylated Isotype Control

Ctrl IC013B
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Staining Reagents
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Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

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Supplemental ELISA Products
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Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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