Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 205115
View all Glut2 Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with mouse Glut2
Met1-Val523
Accession # P14246

Specificity

Detects mouse Glut2. Stains mouse Glut2-transfected NS0 cells but not NS0 control transfectants. It also detects Glut2 on mouse insulinoma beta TC-6 cells (2).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Glut2 Biotinylated Antibody

Glut2 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC).

Glut2 in beta TC‑6 Mouse Cell Line.

Glut2 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Rat Anti-Mouse Glut2 Biotinylated Monoclonal Antibody (Catalog # BAM1440) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow, upper panel; Catalog # NL013) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Mouse Glut2 Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: beta TC‑6 mouse beta cell insulinoma cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed beta TC-6 mouse beta cell insulinoma cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney and pancreatic beta -cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta -cells. Glut2 facilitates either glucose uptake or efflux from cells depending on the nutritional state (1).

References

  1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
  2. Poitout, V. et al. (1995) Diabetes 44:306.

Long Name

Glucose Transporter Type 2

Alternate Names

SLC2A2

Entrez Gene IDs

6514 (Human); 20526 (Mouse)

Gene Symbol

SLC2A2

UniProt

P14246

Additional Glut2 Products

Product Documents for Mouse Glut2 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Glut2 Biotinylated Antibody

For research use only

Related Research Areas

Diabetes
Glucose Homeostasis
Metabolism

Citations for Mouse Glut2 Biotinylated Antibody

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Protocols

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