Mouse Gremlin Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Gremlin in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant chicken Caronte, recombinant human (rh) Cerberus, rhCOCO, rhGremlin, rhBMP-2, rhBMP-4, recombinant mouse (rm) DAN, rmPRDC, rmSlit1, rmSlit2, or rmBMP-4 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 155606
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Gremlin
    Lys25-Asp184
    Accession # O70326
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
    • Mouse Gremlin Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse Gremlin Antibody (Catalog # MAB956 )
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Gremlin Biotinylated Antibody (Catalog # BAF956 )
  • ELISA Standard
     
    Recombinant Mouse Gremlin Protein (Catalog # 956-GR )
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Gremlin

Gremlin was identified in a Xenopus expression-cloning screen as a dorsalizing factor that can induce a secondary axis. A rat homolog, called Drm, was identified as a cDNA that was downregulated in v-mos transfected cells. Gremlin/Drm belongs to the DAN family of secreted glycoproteins that are BMP antagonists. Other members of the family include: Cerberus, Dante, PRDC, Caronte, and DAN. DAN family members share a cysteine-rich domain that is structurally related to the cysteine-knot motif found in TGF-beta superfamily ligands. In vitro, Gremlin/Drm binds BMP-4 and BMP-2 indicating that it might interfere with BMP signaling. Gremlin/Drm acts as a BMP-2/4 antagonist in a variety of tissues and developmental processes including: Xenopus animal cap explants, chick limb bud outgrowth and chondrogenesis, murine lung branching morphogenesis, and osteogenic differentiation of mouse myoblasts and bone marrow stromal cells. In addition, expression of Gremlin/Drm has been shown to be downregulated in a wide range of human cancer cell lines. Mouse, human, chick and Xenopus homologs of Gremlin share over 80% amino acid identity. It is likely that various DAN family members and other BMP antagonists including Noggin, Chordin, Follistatin and TSG can selectively antagonize the activities of different subsets of TGF-beta superfamily ligands.

  • References:
    1. Hsu, D.R. et al. (1998) Mol. Cell 1:673.
    2. Merino, R. et al. (1999) Development 126:5515.
    3. Shi, W. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 280:L1030.
    4. Topol, L.Z. et al. (2000) J. Biol. Chem. 275:8785.
  • Entrez Gene IDs:
    26585 (Human); 23892 (Mouse)
  • Alternate Names:
    Cell proliferation-inducing gene 2 protein; CKTSF1B1gremlin 1, cysteine knot superfamily, homolog; Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis); DAN domain family member 2; DAND2; DAND2GREMLIN; Down-regulated in Mos-transformed cells protein; DRM; DRMMGC126660; GREM1; gremlin 1; gremlin 1-like protein; Gremlin; gremlin-1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; proliferation-inducing gene 2
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 93  
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Normal Rat IgG Control

Ctrl 6-001-A 11
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB , Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL013 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 3  
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL015 1
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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