Mouse HAI-1 Antibody

Catalog # Availability Size / Price Qty
MAB1141
MAB1141-SP
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Mouse HAI-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HAI-1 in Western blots and direct ELISAs. In Western blots, no cross-reactivity with recombinant mouse (rm) HAI-2B or rmHAI-2A is observed.
Source
Monoclonal Rat IgG2A Clone # 199732
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443
Accession # Q9R097
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse HAI‑1 (Catalog # 1141-PI) under non-reducing conditions only

Mouse HAI-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse HAI-1 Biotinylated Antibody (Catalog # BAF1141)

Standard: Recombinant Mouse HAI-1 Protein, CF (Catalog # 1141-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References
  1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
  2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Entrez Gene IDs
6692 (Human); 20732 (Mouse)
Alternate Names
HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1

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