Key Product Details

Species Reactivity

Mouse

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 744509
View all HSF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HSF1
Thr124-Tyr247
Accession # P38532

Specificity

Detects mouse HSF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HSF2 or recombinant mouse HSF4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse HSF1 Antibody

HSF1 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr).

HSF1 in Mouse Testis.

HSF1 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse HSF1 Monoclonal Antibody (Catalog # MAB7130) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse HSF1 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse testis

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSF1

Heat Shock Factor 1 (HSF1) is a 75-80 kDa constitutively expressed protein that functions as a transcriptional regulator under heat shock conditions. Heat stress induces the serine hyperphosphorylation, sumoylation, trimerization, and translocation of HSF1 to the nucleus. Nuclear HSF1 binds to heat shock elements (HSE) and promotes the transcription of genes important in cell protection from heat stress. Mouse HSF1 contains a DNA binding domain (aa 16-120), two hydrophobic oligomerization motifs (aa 137-212 and aa 378-407), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). Alternate splicing of mouse HSF1 generates a short isoform that lacks 22 amino acids of the transactivation domain. Within aa 124-247, mouse HSF1 shares 94% and 99% aa sequence identity with human and rat HSF1, respectively.

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Entrez Gene IDs

3297 (Human); 154991 (Mouse); 79245 (Rat)

Gene Symbol

HSF1

UniProt

P38532 (Mouse)

Additional HSF1 Products

Product Documents for Mouse HSF1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse HSF1 Antibody

For research use only

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Protocols

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