Mouse HSF1 Antibody

Catalog # Availability Size / Price Qty
MAB7130
MAB7130-SP
HSF1 in Mouse Testis.
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Mouse HSF1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HSF1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human HSF2 or recombinant mouse HSF4 is observed.
Source
Monoclonal Rat IgG2A Clone # 744509
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse HSF1
Thr124-Tyr247
Accession # P38532
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry HSF1 antibody in Mouse Testis by Immunohistochemistry (IHC-Fr). View Larger

HSF1 in Mouse Testis. HSF1 was detected in perfusion fixed frozen sections of mouse testis using Rat Anti-Mouse HSF1 Monoclonal Antibody (Catalog # MAB7130) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei of spermatocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSF1

Heat Shock Factor 1 (HSF1) is a 75-80 kDa constitutively expressed protein that functions as a transcriptional regulator under heat shock conditions. Heat stress induces the serine hyperphosphorylation, sumoylation, trimerization, and translocation of HSF1 to the nucleus. Nuclear HSF1 binds to heat shock elements (HSE) and promotes the transcription of genes important in cell protection from heat stress. Mouse HSF1 contains a DNA binding domain (aa 16-120), two hydrophobic oligomerization motifs (aa 137-212 and aa 378-407), a regulatory domain (aa 221-310), and a transactivation domain (aa 367-525). Alternate splicing of mouse HSF1 generates a short isoform that lacks 22 amino acids of the transactivation domain. Within aa 124-247, mouse HSF1 shares 94% and 99% aa sequence identity with human and rat HSF1, respectively.

Long Name
Heat Shock Transcription Factor 1
Entrez Gene IDs
3297 (Human); 154991 (Mouse); 79245 (Rat)
Alternate Names
heat shock factor protein 1; heat shock transcription factor 1HSTF1HSF 1; HSF1; HSTF 1; HSTF1

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