Mouse ICOS PE-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse ICOS in direct ELISAs.
  • Source
    Monoclonal Rat IgG2B Clone # 670306
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse ICOS
    Accession # Q9WVS0
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of ICOS in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with 1 μg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS PE‑conjugated Monoclonal Antibody (Catalog # FAB168P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ICOS

Mouse ICOS (Inducible Co-Stimulator), also known as AILIM (Activiation-Inducible Lymphocyte Immunomediatory Molecule) and CRP-1 (CD28-Related Protein-1), is a member of the CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Mouse ICOS is a homodimeric type I transmembrane protein consisting of 200 amino acids (aa) with a putative 20 aa signal sequence, a 122 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain. ICOS shares approximately 39% aa similarity with CD28 and CTLA-4. Mouse and human ICOS share approximately 72% aa identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is upregulated within approximately 24-48 hours of activation on Th primed cells. B7-H2, a member of the B7 family of costimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

  • References:
    1. Coyle, A.J. and J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
    2. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040.
    3. Yoshinaga, S.K. et al. (1999) Nature 402:827.
    4. Hutloff, A. et al. (1999) Nature 397:263.
    5. Aicher, A. et al. (2000) J. Immunol. 164:4689.
    6. Coyle, A.J. et al. (2000) Immunity 13:95.
    7. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1.
  • Long Name:
    Inducible Co-stimulator
  • Entrez Gene IDs:
    29851 (Human); 54167 (Mouse)
  • Alternate Names:
    Activation-inducible lymphocyte immunomediatory molecule; AILIM; AILIMCD278; CD278 antigen; CD278; CRP-1; CVID1; inducible costimulator; inducible T-cell costimulator; inducible T-cell co-stimulator; MGC39850
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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