Mouse IFN-alpha 2/IFNA2 Antibody

Catalog #: MAB101491 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB101491-100
MAB101491-SP

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Mouse IFN-alpha 2/IFNA2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IFN-alpha 2/IFNA2 in direct ELISAs. In direct ELISAs, no cross-reactivity with mouse IFN-alpha 1, 4, 9, 12 and 15 was observed.
Source
Monoclonal Rat IgG1 Clone # 996548
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2
Cys24-Glu190
Accession # P01573
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA
This antibody functions as an ELISA detection antibody when paired with mouse anti-IFNA-2 Monoclonal Antibody (Catalog # MAB10149).This product is intended for assay development on various assay platforms requiring antibody pairs. 
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 2/IFNA2

Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).

References
  1. Pestka, S. (2007) J Biol Chem. 282:20047.
  2. Paul, F. et al. (2015) Gene. 567(2):132.
  3. Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
  4. Pesch, V. et al. (2004). Journal of Virology, 78:8219.
  5. Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
  6. Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.
Long Name
Interferon alpha 2
Entrez Gene IDs
3440 (Human); 15965 (Mouse); 298213 (Rat)
Alternate Names
alpha-2a interferon; Ifa2; IFNA; IFNA2; IFNA2a; IFNA2b; IFNA2c; IFNAA; IFNalpha 2; IFN-alpha 2; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765

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