Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IFN-kappa Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse IFN-kappa
Leu22-Lys199 (Cys174Tyr)
Accession # Q7TSL0

Specificity

Detects mouse IFN-kappa in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse IFN-kappa Antibody

Detection of Mouse IFN-? antibody by Western Blot.

Detection of Mouse IFN-kappa by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN-kappa at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of Mouse IFN-? antibody by Simple WesternTM.

Detection of Mouse IFN‑ kappa by Simple WesternTM.

Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL-4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN-kappa at approximately 40 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Mouse IFN-kappa Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line and EL‑4 mouse lymphoblast cell line

Western Blot

1 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL‑4 mouse lymphoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-kappa

IFN-kappa (Interferon kappa) is a secreted, nonglycosylated polypeptide that belongs to the IFN-kappa subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-kappa induces TNF-alpha, IL-10 and MCP-1 production by monocytes. Mouse IFN-kappa precursor is 199 amino acids (aa) in length. It contains a 21 aa signal sequence, plus a 178 aa mature region. There are two intrachain disulfide bonds plus a fifth unpaired cysteine. Mature mouse IFN-kappa (aa 22‑199) shares 68% and 30% aa identity with rat and human IFN-kappa, respectively.

Long Name

Interferon kappa

Alternate Names

IFNK, IFNkappa

Entrez Gene IDs

56832 (Human); 387510 (Mouse)

Gene Symbol

IFNK

UniProt

Q7TSL0

Additional IFN-kappa Products

Product Documents for Mouse IFN-kappa Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IFN-kappa Antibody

For research use only

Related Research Areas

Interferons

Citations for Mouse IFN-kappa Antibody

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