Mouse IFN-kappa Antibody
Mouse IFN-kappa Antibody Summary
Accession # Q7TSL0
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse IFN-kappa by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL-4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN-kappa at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Detection of Mouse IFN‑ kappa by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL-4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN-kappa at approximately 40 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IFN-kappa (Interferon kappa) is a secreted, nonglycosylated polypeptide that belongs to the IFN-kappa subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-kappa induces TNF-alpha, IL-10 and MCP-1 production by monocytes. Mouse IFN-kappa precursor is 199 amino acids (aa) in length. It contains a 21 aa signal sequence, plus a 178 aa mature region. There are two intrachain disulfide bonds plus a fifth unpaired cysteine. Mature mouse IFN-kappa (aa 22‑199) shares 68% and 30% aa identity with rat and human IFN-kappa, respectively.
Citation for Mouse IFN-kappa Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity
Authors: AC Billi, M Gharaee-Ke, J Fullmer, LC Tsoi, BD Hill, D Gruszka, J Ludwig, X Xing, S Estadt, SJ Wolf, SM Rizvi, CC Berthier, JB Hodgin, MA Beamer, MK Sarkar, Y Liang, R Uppala, S Shao, C Zeng, PW Harms, ME Verhaegen, JJ Voorhees, F Wen, NL Ward, AA Dlugosz, JM Kahlenberg, JE Gudjonsson
JCI Insight, 2019-04-18;4(8):.
Sample Types: Whole Cells
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