Mouse IGF-II/IGF2 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF792
Product Details
Citations (2)
FAQs
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Mouse IGF-II/IGF2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGF-II/IGF2 in ELISAs and Western blots. In sandwich immunoassays, less than 4% cross-reactivity with recombinant human (rh) IGF-II is observed and less than 0.1% cross-reactivity with rhIGF-I and recombinant mouse IGFBP1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IGF-II/IGF2
Ala25-Glu91
Accession # P09535
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IGF-II/IGF2 (Catalog # 792-MG)

Mouse IGF-II/IGF2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse IGF-II/IGF2 Antibody (Catalog # MAB792)

Standard: Recombinant Mouse IGF-II/IGF2 Protein, CF (Catalog # 792-MG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-II/IGF2

IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). Evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112). This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15). There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor. This is distinct from IGF-II, is secreted by pancreatic b‑cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners. It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1-6 (18, 19). 

References
  1. LeRoith, D. & C.T. Roberts Jr. (2003) Cancer Lett. 195:127.
  2. Werner, H. & D. LeRoith (2000) Cell. Mol. Life Sci. 57:932.
  3. Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511.
  4. Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687.
  5. Rotwein, P. & L.J. Hall (1990) DNA Cell Biol. 10:725.
  6. Chao, W. & P.A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111.
  7. Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265.
  8. Goya, L. et al. (1999) J. Biol. Chem. 274:24633.
  9. McCarthy, T.L. et al. (1992) Endocrinology 130:1303.
  10. Zindy, F. et al. (1992) J. Hepatol. 14:30.
  11. Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77.
  12. Merrick, D. et al. (2007) BMC Dev. Biol. 7:65.
  13. Stempien, M.M. et al. (1986) DNA 5:357.
  14. Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855.
  15. Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823.
  16. Buchanan, C.M. et al. (2001) Biochem. J. 360:431.
  17. Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117.
  18. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
  19. Belfiore, A. (2007) Curr. Pharm. Des. 13:671.
Long Name
Insulin-like Growth Factor II
Entrez Gene IDs
3481 (Human); 16002 (Mouse)
Alternate Names
C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; GRDF; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PEG2; PP9974; somatomedin-A

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Citations for Mouse IGF-II/IGF2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation.
    Authors: Ostrovsky O, Ahmed NT, Argon Y
    Mol. Biol. Cell, 2009-01-21;20(6):1855-64.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  2. Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
    Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
    Clin. Cancer Res., 2007-02-01;13(3):1000-9.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development

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