Mouse IGF-II/IGF2 Antibody Summary
Accession # P09535
Mouse IGF-II/IGF2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Recombinant Human and Mouse IGF-II/IGF2 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG), Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) and Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IGF-II/IGF2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112). This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15). There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor. This is distinct from IGF-II, is secreted by pancreatic B cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners. It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1-6 (18, 19). The first three receptors initiate downstream signaling events, the IGF-2R sequesters local IGF‑II, and the six IGFBPs regulate IGF-II activity in various tissues.
- LeRoith, D. & C.T. Roberts Jr. (2003) Cancer Lett. 195:127.
- Werner, H. & D. LeRoith (2000) Cell. Mol. Life Sci. 57:932.
- Pavelic, J. et al. (2007) Indian J. Med. Res. 125:511.
- Varela-Nieto, I. et al. (2007) Curr. Pharm. Des. 13:687.
- Rotwein, P. & L.J. Hall (1990) DNA Cell Biol. 10:725.
- Chao, W. & P.A. D-Amore (2008) Cytokine Growth Factor Rev. 19:111.
- Rotwein, P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:265.
- Goya, L. et al. (1999) J. Biol. Chem. 274:24633.
- McCarthy, T.L. et al. (1992) Endocrinology 130:1303.
- Zindy, F. et al. (1992) J. Hepatol. 14:30.
- Holthuizen, P.E. et al. (1993) Regul. Pept. 48:77.
- Merrick, D. et al. (2007) BMC Dev. Biol. 7:65.
- Stempien, M.M. et al. (1986) DNA 5:357.
- Ostrovsky, O. et al. (2009) Mol. Biol. Cell 20:1855.
- Daughaday, W.H. et al. (1993) Proc. Natl. Acad. Sci. USA 90:5823.
- Buchanan, C.M. et al. (2001) Biochem. J. 360:431.
- Cornish, J. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292:E117.
- Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
- Belfiore, A. (2007) Curr. Pharm. Des. 13:671.
Citations for Mouse IGF-II/IGF2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis
Authors: C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini
BMC Cancer, 2019;19(1):126.
Sample Types: Recombinant Protein
Applications: Western Blot
Reveglucosidase alfa (BMN 701), an IGF 2 Tagged rhAcid ?-Glucosidase, Improves Respiratory Functional Parameters in a Murine Model of Pompe Disease
Authors: Laurie Tsuruda
J. Pharmacol. Exp. Ther., 2016;0(0):.
Sample Types: Plasma
Applications: Functional Assay
The chaperone activity of GRP94 toward insulin-like growth factor II is necessary for the stress response to serum deprivation.
Authors: Ostrovsky O, Ahmed NT, Argon Y
Mol. Biol. Cell, 2009;20(6):1855-64.
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy.
Authors: Yin D, Vreeland F, Schaaf LJ, Millham R, Duncan BA, Sharma A
Clin. Cancer Res., 2007;13(3):1000-9.
Sample Types: Plasma
Applications: ELISA Development
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