Human ITFG1 Antibody

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Detection of ITFG1 in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Human ITFG1 Antibody Summary

Species Reactivity
Detects human ITFG1 in direct ELISAs.
Monoclonal Mouse IgG2B Clone # 936213
Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK293-derived human ITFG1
Accession # Q8TB96
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of ITFG1 antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of ITFG1 in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB89001, filled histogram) or isotype control antibody (MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Staining was performed using our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ITFG1

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology.  A ubiquitously expressed 98 kDa glycoprotein, ITFG1 contains an N-terminal signal peptide and a C-terminal transmembrane domain flanking twelve potential N-linked glycosylation sites.  Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model.  Over amino acids (aa) 1-566, human ITFG1 shares 89% and 88% aa identity with mouse and rat ITFG1, respectively.

Long Name
Integrin Alpha FG-GAP Repeat Containing 1
Entrez Gene IDs
81533 (Human); 71927 (Mouse); 171083 (Rat)
Alternate Names
CDA08; integrin alpha FG-GAP repeat containing 1,2310047C21Rik; Integrin-alpha FG-GAP repeat-containing protein 1; ITFG1; Protein TIP; T-cell immunomodulatory protein; TIP; TIPT cell immunomodulatory protein

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Citation for Human ITFG1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. LINKIN-associated proteins necessary for tissue integrity during collective cell migration
    Authors: CH Tan, KW Cheng, H Park, PW Sternberg, TF Chou
    bioRxiv : the preprint server for biology, 2023-06-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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