Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 936213
View all ITFG1 Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived human ITFG1
Met1-Ile566
Accession # Q8TB96

Specificity

Detects human ITFG1 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human ITFG1 Antibody

Detection of ITFG1 antibody in K562 Human Cell Line antibody by Flow Cytometry.

Detection of ITFG1 in K562 Human Cell Line by Flow Cytometry.

K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human ITFG1 Monoclonal Antibody (Catalog # MAB89001, filled histogram) or isotype control antibody (MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Staining was performed using our protocol for Staining Membrane-associated Proteins.

Applications for Human ITFG1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: K562 human chronic myelogenous leukemia cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ITFG1

Integrin-alpha FG-GAP repeat-containing protein 1 (ITFG1), also known as T cell immunomodulatory protein (TIP), was initially identified using bioinformatics and high-throughput cell-based screening assays to isolate novel factors involved in T cell biology.  A ubiquitously expressed 98 kDa glycoprotein, ITFG1 contains an N-terminal signal peptide and a C-terminal transmembrane domain flanking twelve potential N-linked glycosylation sites.  Human and mouse T cells treated with ITFG1 in vitro secreted the cytokines IFN-g, TNF-a and IL-10, while in vivo ITFG1 was protective in a mouse acute graft-versus-host disease (GVHD) model.  Over amino acids (aa) 1-566, human ITFG1 shares 89% and 88% aa identity with mouse and rat ITFG1, respectively.

Long Name

Integrin Alpha FG-GAP Repeat Containing 1

Alternate Names

CDA08, TIP

Entrez Gene IDs

81533 (Human); 71927 (Mouse); 171083 (Rat)

Gene Symbol

ITFG1

UniProt

Q8TB96

Additional ITFG1 Products

Product Documents for Human ITFG1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human ITFG1 Antibody

For research use only

Citations for Human ITFG1 Antibody

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